Selection of a method of condensed tannin analysis for studies with rumen bacteria

Bae, Hwallip; McAllister, Tim A.; Muir, Alister D.; Yanke, L. J.; Bassendowski, K. A.; Cheng, K.-J.

doi:10.1021/jf00032a018

Cited by 25 publications

(20 citation statements)

References 21 publications

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“…Previous studies have indicated a decrease in CH 4 production due to the effects of high CT concentrations associated with a reduction in DM degradability . Condensed tannins could reduce the fiber digestion by complexing with lignocelluloses, thereby suppressing microbial digestion or reducing the number of cellulolytic microorganisms and/or activities of fibrolytic enzymes . In the present study, IVDMD was not significantly reduced by the different MWs of the CT fractions.…”

Section: Discussionsupporting

confidence: 69%

Effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on in vitro methane production and rumen fermentation

et al. 2014

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Section: Discussionsupporting

confidence: 69%

Effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on in vitro methane production and rumen fermentation

et al. 2014

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“…The water solution was centrifuged for 20 min at 2000 g. The resulting extract was diluted 1:1 with methanol and applied to a Sephadex LH-20 column (40 Â 2.5 cm i.d. ; Pharmacia, Uppsala, Sweden), equilibrated with 50% aqueous methanol (Bae et al, 1993). The column was washed with 50% aqueous methanol until the absorbance of the eluate at 280 nm was less than 0.1 AU.…”

Section: Extraction and Purificationmentioning

confidence: 99%

Proanthocyanidins of mountain birch leaves: quantification and properties

Ossipova

Ossipov

Haukioja

et al. 2001

Phytochemical Analysis

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Proanthocyanidins (PAs; condensed tannins) are present in mountain birch leaves in soluble and cell wall-bound forms. Crude preparations of soluble PAs were isolated from birch leaves and purified by chromatography on a Sephadex LH-20 column with a yield of about 7% of leaf dry mass. Some chemical characteristics were elucidated with 13C-NMR and HPLC-ECI-MS. Birch leaf PAs were mainly delphinidin type oligo- and polymers with average molecular mass of about 3000. In order to quantify PAs, the method involving heating PA-containing materials in 1-butanol:hydrochloric acid (95:5, v/v), and spectrophotometric determination of the anthocyanidin monomers so formed was modified and optimised. Mature leaves were characterised by a relatively high content of PAs: mean values for soluble and bound PAs were 103 and 40 mg/g dry mass, respectively. In mature leaves the soluble PAs determined the total protein precipitation capacity (PPC) of extracts. In young leaves, the contribution of PAs to the total content of phenolics and the total PPC of tannins was about 20-25% only.

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“…This leads to the PA concentration that is unrelated to their bioactive effects because the polyphenols can react with the analytical reagent to form PA. In addition, the most popular method for quantifying the total phenolics is prussian blue assay due to its simple, rapid, and low interference by nonphenolic compounds (Graham 1992;Bae et al 1993). However, temperature, pH, and reaction time could affect the color density in this method (Graham 1992).…”

Section: Analytical Methods Of Pamentioning

confidence: 99%

Production and transcriptional regulation of proanthocyanidin biosynthesis in forage legumes

Zhou

Wei

Sun

et al. 2015

Appl Microbiol Biotechnol

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Proanthocyanidins (PA), also known as condensed tannins, contribute to important forage legumes traits including disease resistance and forage quality. PA in forage plants has both positive and negative effects on feed digestibility and animal performance. The analytical methods and their applicability in measuring the contents of PA in forage plants are essential to studies on their nutritional effects. In spite of important breakthroughs in our understanding of the PA biosynthesis, important questions still remain to be answered such as the PA polymerization and transport. Recent advances in the understanding of transcription factor-mediated gene regulation mechanisms in anthocyanin and PA biosynthetic pathway in model plants suggest new approaches for the metabolic engineering of PA in forage plants. The present review will attempt to present the state-of-the-art of research in these areas and provide an update on the production and metabolic engineering of PA in forage plants. We hope that this will contribute to a better understanding of the ways in which PA production to manipulate the content of PA for beneficial effects in forage plants.

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Selection of a method of condensed tannin analysis for studies with rumen bacteria

Cited by 25 publications

References 21 publications

Effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on in vitro methane production and rumen fermentation

Effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on in vitro methane production and rumen fermentation

Proanthocyanidins of mountain birch leaves: quantification and properties

Production and transcriptional regulation of proanthocyanidin biosynthesis in forage legumes

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