Selection of Antibodies with Tailored Properties by Application of High-Throughput Multiparameter Fluorescence-Activated Cell Sorting of Yeast-Displayed Immune Libraries

Abstract

In this study, we present a multiparameter screening procedure for the identification of target-specific antibodies with prescribed properties. Based on B cell receptor gene repertoires from transgenic rats, yeast surface display libraries were generated, and high-affinity human antibodies were readily isolated. We demonstrate that specific desirable features, i.e., species’ cross-reactivity and a broad epitope coverage can be integrated into the screening procedure using high-throughput fluorescence-activated cell sorting. We show that the applied screening stringencies translate directly into binding properties of isolated human antibody variants.Electronic supplementary materialThe online version of this article (10.1007/s12033-018-0109-0) contains supplementary material, which is available to authorized users.

Efficient Display of Aspergillus niger β-Glucosidase on Saccharomyces cerevisiae Cell Wall for Aroma Enhancement in Wine

“…In recent years, the cell-surface-display system by yeast has been widely used in waste biorefineries and antibody screening, with relatively few applications in the food industry. Pan et al constructed a displayed system of lipase on the cell wall of Pichia pastoris , which can be used as a biocatalyst to enrich eicosapentaenoic acid and docosahexaenoic acid content in fish oils .…”