1989
DOI: 10.1007/bf00716663
|View full text |Cite
|
Sign up to set email alerts
|

Selection of bacterial wilt-resistant tomato through tissue culture

Abstract: Bacterial wilt-resistant plants were obtained using a tomato tissue culture system. A virulent strain ofPseudomonas solanacearum secreted some toxic substances into the culture medium. Leaf explant-derived callus tissues which were resistant to these toxic substances in the culture filtrate were selectedin vitro and regenerated into plants. These plants expressed bacterial wilt resistance at the early infection stage to suppress or delay the growth of the inoculated bacteria. On the other hand, complete resist… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
19
1

Year Published

1991
1991
2016
2016

Publication Types

Select...
5
2

Relationship

1
6

Authors

Journals

citations
Cited by 41 publications
(21 citation statements)
references
References 11 publications
1
19
1
Order By: Relevance
“…This last example together with similar results obtained by Brettell et al (1980), Ling et al (1985), Toyoda et al (1989b) and Binarovfi et al (1990), show that an increase in the percentage of resistant regenerants can be expected when a preselection in vitro is applied. For those studies in which selection in vitro did not increase the frequency of resistant regenerants, it is likely that either the toxic compounds used did not play an important role in pathogenesis or the method used for selection was inappropriate, e.g.…”
Section: Selection In Vitro Versus Selection At the Plant Levelsupporting
confidence: 73%
See 3 more Smart Citations
“…This last example together with similar results obtained by Brettell et al (1980), Ling et al (1985), Toyoda et al (1989b) and Binarovfi et al (1990), show that an increase in the percentage of resistant regenerants can be expected when a preselection in vitro is applied. For those studies in which selection in vitro did not increase the frequency of resistant regenerants, it is likely that either the toxic compounds used did not play an important role in pathogenesis or the method used for selection was inappropriate, e.g.…”
Section: Selection In Vitro Versus Selection At the Plant Levelsupporting
confidence: 73%
“…Nevertheless, the occurrence of much higher frequencies, e.g. 36% of unselected tomato regenerants segregating for bacterial wilt resistance in their progenies (Toyoda et al, 1989b), and the failures to detect resistance in other studies emphasize that the relevance of such estimated frequencies is in doubt.…”
Section: Frequency Of Disease Resistant Variantsmentioning
confidence: 97%
See 2 more Smart Citations
“…The regenerated plants were transplanted to soil in plastic pots and acclimated in a moist growth chamber for one week and then placed in a shaded greenhouse for two weeks. Regenerated plants with [4][5] leaves and non-cultured parental tobacco plants (as a control) at the same growth stage were inoculated with a virulent strain U-10 of P. solanacearum which was kindly given by Dr. H. Tanaka, Japan Tobacco Inc. To secure bacterial inoculation, roots of these plants were cut by plunging a razor blade down into the soil (5 cm downward) at positions 2 cm distant from plants in order to help bacterial cell penetration into the vascular system9). The U-10 was shake-cultured to an exponential phase in PCG medium (10 g pepton, 10 g casamino acids, and 1 g glucose in 11 water), collected by centrifugation, and suspended in sterilized water.…”
mentioning
confidence: 99%