Selection of Conditions for Cellulase and Xylanase Extraction from Switchgrass Colonized by Acidothermus cellulolyticus

Abstract

Solid-state fermentation has been widely used for enzyme production. However, secreted enzymes often bind to the solid substrate preventing their detection and recovery. A series of screening studies was performed to examine the role of extraction buffer composition including NaCl, ethylene glycol, sodium acetate buffer, and Tween 80, on xylanase and cellulase recovery from switchgrass. Our results indicated that the selection of an extraction buffer is highly dependent on the nature and source of the enzyme being extracted. While a buffer containing 50 mM sodium acetate at pH 5 was found to have a positive effect on the recovery of commercial fungal-derived cellulase and xylanase amended to switchgrass, the same buffer had a significant negative effect on enzyme extraction from solid fermentation samples colonized by the bacterium Acidothermus cellulolyticus. Xylanase activity was more affected by components in the extraction buffers compared to cellulase. This study demonstrated that extraction followed by diafiltration is important for assessing enzyme recovery from solid fermentation samples. Reduction in activity due to compounds present in the switchgrass extracts is reversible when the compounds are removed via diafiltration.

Improving the extraction conditions of endoglucanase produced by Aspergillus niger under solid-state fermentation

“…The enzymes considered include pectinase (Castilho et al, 2000;Singh et al, 1999), xylanase (Heck et al, 2005;Maciel et al, 2009;Pal and Khanum, 2010;Rezaei et al, 2011), lipase (Menoncin et al, 2010), inulinase (Bender Brazilian Journal of Chemical Engineering et al, 2008;Chen et al, 2011), and manganeseperoxidase (Arantes et al, 2011). However, there have been few studies concerning the optimum conditions for cellulase extraction from SSF cultivations.…”

Genome Sequence of Paenibacillus terrae HPL-003, a Xylanase-Producing Bacterium Isolated from Soil Found in Forest Residue

“…Degradable xylanase as well as cellulase biomass is being recognized as an important enzyme, as xylanase is an indispensable enzyme in biobased industries (4,6,9). Various strains isolated from soil in a variety of locations have been screened to detect bacteria with high xylanase activities.…”

Solid-State Fermentation as a Novel Paradigm for Organic Waste Valorization: A Review

“…They observed that multiple extractions with sonication improved the recovery of enzymes from SSF with an increment of enzyme yield more than twice that of conventional single stage extraction. Furthermore, in order to improve the enzyme recovery from SSF, it has been observed that extraction followed by diafiltration can enhance the activity recovery by removing inhibitory phenolic compounds from extracts [217]. Since the recovery and concentration steps are significant in enzyme production, employing ultrafiltration with 10 kDa cut off after solid-liquid extraction can improve the rate of enzyme recovery by about 74% [20,218].…”