Selection of DNA markers for detection of extreme resistance to potato virus Y in tetraploid potato (Solanum tuberosum L.) F&lt;sub&gt;1&lt;/sub&gt; progenies

Heldák, Ján; Bežo, Milan; Štefúnová, Veronika; Galliková, Andrea

doi:10.17221/2074-cjgpb

Cited by 21 publications

(10 citation statements)

References 28 publications

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“…Consequently, the 13 cultivars that were found to be resistant to both strains of PVY are likely to possess one of the Ry extreme resistance genes. It was likely that at least Ry sto was present, as it has been previously found in the cultivars White Lady and Rioja [15,25,38], which were both found to be phenotypically resistant here.…”

Section: Discussionsupporting

confidence: 68%

“…The phenotypic scores for cultivars from this study are generally consistent with the results reported from other studies. The cultivar White Lady was also reported to be resistant to PVY in four previous studies [15,25,32,38], and Cernák et al [25] also reported Rioja to be resistant. A number of cultivars, including Atlantic [31,39,40], Bliss [37], King Edward [31,39], Kipfler [37], Pike [31], Ranger Russet [19], Russet Burbank [19,31], Shepody [8] and Umatilla [19], were reported to be susceptible elsewhere.…”

Section: Discussionmentioning

confidence: 84%

“…Desiree has been reported to contain the Ny tbr hypersensitivity gene, which is active against PVY O [39,41], and to display hypersensitive reactions of severe necrosis, leaf drop, and stunting [39]. However, Desiree was also described as displaying non-hypersensitive symptoms of severe mosaic and leaf deformation [39], and Desiree has been noted as susceptible in another study that used ELISA to detect the virus [38]. This may indicate that in Desiree, the hypersensitive reaction is not preventing some multiplication and systemic movement of the virus.…”

Section: Discussionmentioning

confidence: 99%

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Screening for Resistance to PVY in Australian Potato Germplasm

Slater

Schultz

Lombardi

et al. 2020

Genes

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Potatoes are an important human food crop, but have a number of yield limiting factors, including disease susceptibility. Potato virus Y (PVY) is found worldwide, and is one of the main virus problems for potato growers. PVY is transmitted by aphids and mechanically by machinery, tools and people, and symptoms are variable across cultivars and strains, including being symptomless in some cultivars. Therefore, breeding resistant cultivars is the best way to control this virus. This study phenotypically screened 74 of the main commercial cultivars and a few other select cultivars grown in Australia, in order to identify sources of resistance to PVY. The cultivars were screened against PVY O and PVY NTN , with 23 out of 71 resistant to PVY O and 13 out of 74 resistant to PVY NTN , and all these 13 were resistant to both strains. When the phenotypic screening was compared to the results listed on the European Cultivated Potato Database, the majority of results were found to be consistent. We then evaluated three molecular markers RYSC3, M45, and STM0003 for the extreme resistance genes Ry adg and Ry sto , to validate the usefulness of the markers for marker-assisted selection (MAS) on Australian germplasm. The degree of correlation between the resistance phenotypes and the RYSC3, M45, and STM0003 markers for Ry adg and Ry sto conferred PVY resistance was determined. Three cultivars amplified the RYSC3 marker, while the M45 marker amplified the same 3 and an additional 9. Of the 12 cultivars, 11 phenotyped as resistant, but 1 was susceptible. The STM0003 marker was amplified from only 2 cultivars that both had resistant phenotypes. The RYSC3, M45, and STM0003 markers were therefore able to identify all the 13 cultivars that were resistant to both strains of PVY. Therefore, these markers will enable the identification of genotypes with resistance to PVY, and enable PVY resistant parents to be used for the development of superior progeny; these genetic markers can be used for MAS in the Australian potato breeding program.Genes 2020, 11, 429 2 of 15 mosaic symptoms on the leaves, and this can create a major risk for the spread of the virus in certified seed schemes [8]. Furthermore, the lack of symptoms in infected plants means that visual inspection will generally be ineffective, and that such plants provide a source of PVY inoculum in production areas. Therefore, breeding resistant cultivars is the best way to control this virus and remove inoculum.An effective potato breeding program aims to breed new cultivars that have traits that are superior or equivalent to those of current cultivars. In order to do this, potential parents need to be identified, which contain genes that control at least 40 desirable traits [9,10]. A list of traits that are important for the breeding of improved cultivars in Australia and their genetic control were listed by Slater et al. [10]. A number of these characters are considered to be controlled by single dominant genes, as they exhibit qualitative phenotypic variation. Simko et al.[11] l...

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

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Screening for Resistance to PVY in Australian Potato Germplasm

Slater

Schultz

Lombardi

et al. 2020

Genes

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“…Similar findings were reported by Flis et al (2005), who were unable to detect any of their markers in this variety. Heldák et al (2007) were also unable to detect the markers reported by Flis in Santé. These authors also attributed their findings to the causes outlined above.…”

Section: Discussionmentioning

confidence: 68%

Development of a locus-specific marker and localization of the Rysto gene based on linkage to a catalase gene on chromosome XII in the tetraploid potato genome

et al. 2008

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As a result of many decades of potato breeding in Keszthely, Hungary, numerous varieties resistant to the pathogens attacking potato have been developed using wild Solanum species as resistance sources. In order to simplify the time-consuming breeding process, the present research project involved the development of a SCAR marker correlating with the Ry sto gene, originating from the Solanum stoloniferun species and conferring extreme resistance to the potato Y virus (PVY), using sequence information from a RAPD marker linked to the gene. The usefulness of the marker was tested on 21 varieties resistant or susceptible to PVY and it was found to provide efficient identification of genotypes carrying the Ry sto gene. Based on its linkage to a new chromosome-specific anchor marker (Cat-in2), identified by means of the intron-targeting method not previously used in potato research, the Ry sto gene was localized on chromosome XII.

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“…Thus, for more efficient use of the Ry gene in potato breeding, the knowledge on the allelic dosage of this gene in the parents available in the breeding program is crucial (Koeyer et al 2010). One way of estimating the allelic dosage of a gene in a particular genotype is by the analysis of the segregation of this gene in the progeny, obtained by test crosses, i.e., with a nuliplex parent (susceptible) (Ribeiro et al 2006, Heldák et al 2007, Ortega and Lopez-Vizcon 2012, John et al 2013). …”

Section: Introductionmentioning

confidence: 99%