Selection of down‐regulated sequences along the monocytic differentiation of leukemic HL60 cells

Herblot, Sabine; Vekris, Antoine; Rouzaut, Ana; Najeme, Farid; Miguel, Carlos de; Bezian, J. H.; Bonnet, Jacques

doi:10.1016/s0014-5793(97)00967-8

Cited by 13 publications

(1 citation statement)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). The late response genes were induced or repressed between 12 and 24 h and their expression either peaked or declined by 48 h. Some of the late genes include differentiation markers such as myeloperoxidase (24), villin (25), vimentin (26) and activin A (27,28).…”

Section: Resultsmentioning

confidence: 99%

Gene expression of TPA induced differentiation in HL-60 cells by DNA microarray analysis

Zheng

Ravatn²,

Lin³

et al. 2002

Nucleic Acids Research

View full text Add to dashboard Cite

The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) is a potent inducer of differentiation in human promyelocytic leukemia cells. Recently, TPA has been successfully administered to patients with myelocytic leukemia and has produced therapeutic effects that led to temporary remission. These studies demonstrated the potential efficacy of TPA in cancer chemotherapy. We now seek to understand the biological effects and molecular mechanisms of differentiation in response to TPA treatment in leukemia cells by expression profiling using DNA microarray. Our results show distinct temporal and coordinated gene changes that are consistent with differentiation and activation of multiple biochemical pathways in HL-60 cells exposed to TPA. Alterations of gene expression in HL-60 cells include various transcription factors, cytokines and protein markers that are consistent with the induction of differentiation elicited by TPA. These temporal patterns of gene expression were abolished or greatly diminished in an HL-60 derived TPA- resistant variant cell line (HL-525), thus revealing transcriptional and consequential biochemical changes that may be required for TPA-induced differentiation. In addition, certain genes were upregulated by TPA in TPA-resistant HL-525 cells but not in TPA-sensitive HL-60 cells suggesting that these genes may play a role in the resistant phenotype. These patterns of gene expression may be important for predicting response to TPA.

show abstract