Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling

Kantawong, Fahsai; Burgess, Karl; Jayawardena, K. G. I.; Hart, Andrew; Burchmore, Richard; Gadegaard, Nikolaj; Oreffo, Richard O.C.; Dalby, Matthew J.

doi:10.1016/j.biomaterials.2009.05.040

Cited by 84 publications

(61 citation statements)

References 44 publications

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“…For example, Biggs et al [110] observed reduced adhesion maturation and spreading of osteoprogenitor cells cultivated on nano-pit arrays correlated with impaired osteospecific differentiation through a depression of ERK signalling and downregulation of osteocalcin synthesis. Genetic and proteomic studies of osteoprogenitor cells on disordered nano-pit arrays indicated the involvement of integrin and cytoskeletal signalling along with the ERK1/2 pathway in osteogenesis [111,112]. Similar findings were observed by constraining cell shape using patterns of adhesive islands, which proved to be a very effective means of modulating cell contractility.…”

Section: Cell Instructive Materialssupporting

confidence: 56%

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Ventre

Causa

Netti

2012

J. R. Soc. Interface.

159

153

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The development of novel biomaterials able to control cell activities and direct their fate is warranted for engineering functional biological tissues, advanced cell culture systems, single-cell diagnosis as well as for cell sorting and differentiation. It is well established that crosstalk at the cell–material interface occurs and this has a profound influence on cell behaviour. However, the complete deciphering of the cell–material communication code is still far away. A variety of material surface properties have been reported to affect the strength and the nature of the cell–material interactions, including biological cues, topography and mechanical properties. Novel experimental evidence bears out the hypothesis that these three different signals participate in the same material–cytoskeleton crosstalk pathway via adhesion plaque formation dynamics. In this review, we present the relevant findings on material-induced cell response along with the description of cell behaviour when exposed to arrays of signals—biochemical, topographical and mechanical. Finally, with the aid of literature data, we attempt to draw unifying elements of the material–cytoskeleton–cell fate chain.

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Section: Cell Instructive Materialssupporting

confidence: 56%

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Ventre

Causa

Netti

2012

J. R. Soc. Interface.

159

153

View full text Add to dashboard Cite

show abstract

“…41,42 Many studies have shown that nanotopographies dictate the differentiation activity of stem cells by influencing the phosphorylation of these two kinases. 43,44 Recent work has also demonstrated that the integrin-linked kinase/β-catenin pathway is important in mediating signals from topographical cues to direct osteogenic differentiation of cells. 45 The exact role of hybrid micro/nanorod topography in the response of the cell remains to be determined.…”

Section: Osteogenic Differentiation Activity In Bone Marrow Mscsmentioning

confidence: 99%

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Zhang¹,

Li²,

Huang³

et al. 2013

IJN

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Background and methods:Various methods have been used to modify titanium implant surfaces with the aim of achieving better osseointegration. In this study, we fabricated a clustered nanorod structure on an acid-etched, microstructured titanium plate surface using hydrogen peroxide. We also evaluated biofunctionalization of the hybrid micro/nanorod topography on rat bone marrow mesenchymal stem cells. Scanning electron microscopy and x-ray diffraction were used to investigate the surface topography and phase composition of the modified titanium plate. Rat bone marrow mesenchymal stem cells were cultured and seeded on the plate. The adhesion ability of the cells was then assayed by cell counting at one, 4, and 24 hours after cell seeding, and expression of adhesion-related protein integrin β1 was detected by immunofluorescence. In addition, a polymerase chain reaction assay, alkaline phosphatase and Alizarin Red S staining assays, and osteopontin and osteocalcin immunofluorescence analyses were used to evaluate the osteogenic differentiation behavior of the cells. Results:The hybrid micro/nanoscale texture formed on the titanium surface enhanced the initial adhesion activity of the rat bone marrow mesenchymal stem cells. Importantly, the hierarchical structure promoted osteogenic differentiation of these cells. Conclusion: This study suggests that a hybrid micro/nanorod topography on a titanium surface fabricated by treatment with hydrogen peroxide followed by acid etching might facilitate osseointegration of a titanium implant in vivo.

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“…24 More recent research has shown a broad range of cell responses to nanotopography. [25][26][27] Nanostructures have recently become of particular interest due to their advantageous high surfaceto-volume ratio. These structures have the potential to elicit additional gratifying biological effects on stem cells.…”

Section: Introductionmentioning

confidence: 99%

Biofunctionalization of a titanium surface with a nano-sawtooth structure regulates the behavior of rat bone marrow mesenchymal stem cells

Zhang¹,

Li²,

Liu³

et al. 2012

IJN

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Background:The topography of an implant surface can serve as a powerful signaling cue for attached cells and can enhance the quality of osseointegration. A series of improved implant surfaces functionalized with nanoscale structures have been fabricated using various methods. Methods: In this study, using an H 2 O 2 process, we fabricated two size-controllable sawtooth-like nanostructures with different dimensions on a titanium surface. The effects of the two nanosawtooth structures on rat bone marrow mesenchymal stem cells (BMMSCs) were evaluated without the addition of osteoinductive chemical factors. Results: These new surface modifications did not adversely affect cell viability, and rat BMMSCs demonstrated a greater increase in proliferation ability on the surfaces of the nanosawtooth structures than on a control plate. Furthermore, upregulated expression of osteogenicrelated genes and proteins indicated that the nano-sawtooth structures promote osteoblastic differentiation of rat BMMSCs. Importantly, the large nano-sawtooth structure resulted in the greatest cell responses, including increased adhesion, proliferation, and differentiation. Conclusion: The enhanced adhesion, proliferation, and osteogenic differentiation abilities of rat BMMSCs on the nano-sawtooth structures suggest the potential to induce improvements in bonetitanium integration in vivo. Our study reveals the key role played by the nano-sawtooth structures on a titanium surface for the fate of rat BMMSCs and provides insights into the study of stem cellnanostructure relationships and the related design of improved biomedical implant surfaces.

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Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling

Cited by 84 publications

References 44 publications

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Biofunctionalization of a titanium surface with a nano-sawtooth structure regulates the behavior of rat bone marrow mesenchymal stem cells

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