Selection of fat-degrading microorganisms for the treatment of lipid-contaminated environment

Abstract

To develop eff ective microbial agents applicable to complex technology for grease wastes utilization, a total of 124 microorganisms were screened for their ability to degrade lipidic compounds. Th e screening yielded fi ve strains (UP2, F2, E13, Kl1 and N3) showing lipolytic activity and rapidly degrading sunfl ower and olive oil, tallow and lard. Among them, strains E13 and N3 were found to have the highest lipase activity and the more intensive rates of the degradation of saturated (palmitic and stearic) and unsaturated (oleic and linoleic) fatty acids and triglycerides containing these fatty acids. Th e strains were obtained from the culture collection of JSC "Biocentras" and identifi ed as Enterobacter aerogenes E13 and Arthrobacter sp. N3. When a mixed culture of strains E. aerogenes E13 and Arthrobacter sp. N3 was grown in a mineral medium containing 0.5% of sunfl ower oil, the hydrolysis products were diglycerides, monoglycerides and free fatty acids. Moreover, the mixed culture was consuming these hydrolysis products during cultivation at 30 °C. Investigation of lard biodegradation in black soil showed that a mixed culture of strains E. aerogenes E13 and Arthrobacter sp. N3 degraded lard about 7 times more intensively than indigenous soil microorganisms (aft er six weeks of degradation in black soil, the concentration of lard was reduced by 87.5 and 8.0%, respectively). Th erefore, the mixed culture of strains E. aerogenes E13 and Arthrobacter sp. N3 may be used for an eff ective grease waste reduction in a complex cleaning technology.

Isolation and Characterization of Indigenous Lipase Producing Bacteria from Lipid?rich Environment

“…occupied the highest number (13) of the total isolates. Cipinyte et al (2009) As the world oil demand is increasing, generation of oil is also increasing. There is a need of appropriate waste minimization or recycling technology which should be easy to operate and cost effective using the microbial resources.…”

Bovine bile as a bio-surfactant pre-treatment option for anaerobic digestion of high-fat cattle slaughterhouse waste

“…Bio-surfactants are naturally-derived, typically non-toxic, and biodegradable surface active agents which improve the solubility of lipids into an aqueous solution, thereby increasing the interaction between microbial enzymes and lipids, and consequently enhancing hydrolysis, the rate-limiting step of anaerobic digestion [6][7][8]. However, this also increases the risk of foaming [9,10].…”

Rhodococcus UKMP-5M, an endogenous lipase producing actinomycete from Peninsular Malaysia

“…Lipids -fat, oil, greases and fatty acids (FAs) -hold largest portion of organic components in municipal and industrial wastewater particularly from food processing industries (Prasad & Manjanuth 2011). The deposited lipids have led to serious anthropogenic damages, such as clogging pipes, congest treatment filters, odour problems, oil film formation, depleting oxygen hence damaging aquatic livings (Cipinyte et al 2009;Fadile et al 2011). This situation gets even worse in presence of great numbers of food industries, restaurants, and slaughterhouses where high amount of fat, oil and greases are being disposed to the waste streams continuously (Bhumibamon et al 2002).…”

“…Aerobic biodegradation processes by active lipolytic strains are regarded as slower and less viable on highly polluted fat contaminants. However, this strategy was proven to be feasible with many other added advantages compared to other strategy (Cipinyte et al 2009). Several studies on enzymatic aerobic treatment have annotated that this strategy promises better effluent quality and does not develop odour problems (Chan et al 2008).…”