Selection of highly fertilization-competent bovine spermatozoa through adhesion to the Fallopian tube epithelium in vitro

Gualtieri, Roberto; Talevi, Riccardo

doi:10.1530/rep.0.1250251

Cited by 65 publications

(37 citation statements)

References 18 publications

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“…There is also evidence from in vitro studies that sulphated glycoconjugates such as heparin can act on oviduct tissues to release sperm from binding (Talevi and Gualtieri, 2001;Gualtieri and Talevi, 2003;Gualtieri et al, 2005). However, very much in line with the overall theme of this essay, the concentrations of heparin or heparin-like glycoconjugates in oviduct luminal fluid are under cyclic ovarian control, reaching a peak during the period of oestrus (Parrish et al, 1989), so one returns to the perspective of gonadal coordination of pre-fertilisation events.…”

Section: Catecholamine and Ca 2þ Ionmentioning

confidence: 77%

“…Viewed at the molecular level, a remodelling of plasma membranes in different sub-populations of spermatozoa and in corresponding regions of the epithelium would be anticipated. Despite this emphasis on physiological control mechanisms, none of the preceding remarks is intended to deny that in-vitro binding assays using oviduct epithelial cells may offer a valuable new route towards sperm quality assessment, that is, a form of screening for potential fertility (De Pauw et al, 2002;Gualtieri and Talevi, 2003;Waberski et al, 2005Waberski et al, , 2006Petrunkina et al, 2007).…”

Section: In Vitro Models Of Sperm Bindingmentioning

confidence: 99%

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Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Hunter

2007

Molecular Reproduction Devel

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To avoid inappropriate conclusions being drawn from the extensive use of in vitro preparations of sperm-oviduct epithelial binding, it is recalled that events in the genital tract of mammals are regulated by the gonads, primarily by their changing secretion of steroid hormones. Key observations from in vivo models are used to emphasise the dynamic interactions between viable sperm cells and the caudal (distal) portion of the oviduct isthmus, the site of the functional sperm reservoir. These include (1) preovulatory arrest and epithelial binding of intact sperm cells and thereby suppression of completion of capacitation, (2) peri-ovulatory activation and release from binding of discrete sub-populations of competent spermatozoa, and (3) post-ovulatory liberation of large numbers of spermatozoa. These observations underline the influence of endocrine regulation of sperm binding and release by peri-ovulatory Graafian follicles, a point brought out by the enhanced sperm release prompted by diverse treatments with solutions of progesterone. In the light of this evidence, the suitability of in vitro preparations for clarifying physiological events should be questioned, especially if myosalpingeal catecholamines diffusing out of the autonomic nervous system contribute to sperm activation and/or release. None of this is to infer that sperm cells themselves are without influence on their epithelial binding reaction(s). Nor is it to suggest that in vitro models of sperm-oviduct binding are without relevance to the development of sperm evaluation technologies. However, pre-ovulatory sperm-epithelial binding and a regulated peri-ovulatory release should be seen as vital tactics in the overall strategy of achieving successful monospermic fertilisation. Mol. Reprod. Dev. 75: 167-174, 2008.

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Section: Catecholamine and Ca 2þ Ionmentioning

confidence: 77%

Section: In Vitro Models Of Sperm Bindingmentioning

confidence: 99%

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Hunter

2007

Molecular Reproduction Devel

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“…It has been reported that, in different species, particular sperm subpopulations are selected by cell-cell adhesion in in vitro cultures with oviductal cells. Particularly in bovines, in vitro selected sperm are characterized by fertilization competence (Gualtieri & Talevi 2003).…”

Section: Introductionmentioning

confidence: 99%

The endocannabinoid system in bull sperm and bovine oviductal epithelium: role of anandamide in sperm–oviduct interaction

Gervasi¹,

Rapanelli²,

Ribeiro³

et al. 2009

Reproduction

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Anandamide binds to cannabinoid receptors and plays several central and peripheral functions. The aim of this work was to study the possible role for this endocannabinoid in controlling sperm-oviduct interaction in mammals. We observed that bull sperm and bovine oviductal epithelial cells express cannabinoid receptors, CB1 and CB2, and fatty acid amide hydrolase, the enzyme that controls intracellular anandamide levels. A quantitative assay to determine whether anandamide was involved in bovine sperm-oviduct interaction was developed. R(C)-methanandamide, a non-hydrolysable anandamide analog, inhibited sperm binding to and induced sperm release from oviductal epithelia. Selective CB1 antagonists (SR141716A or AM251) completely blocked R(C)-methanandamide effects. However, SR144528, a selective CB2 antagonist, did not exert any effect, indicating that only CB1 was involved in R(C)-methanandamide effect. This effect was not caused by inhibition of the sperm progressive motility or by induction of the acrosome reaction. Overall, our findings indicate for the first time that the endocannabinoid system is present in bovine sperm and oviductal epithelium and that anandamide modulates the sperm-oviduct interaction, by inhibition of sperm binding and induction of sperm release from oviductal epithelial cells, probably by activating CB1 receptors.

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“…Bedford and Breed, 1994;Burkman et al, 1984;Overstreet and Cooper, 1975;Overstreet et al, 1980) and motility modulation has also been observed in spermatozoa exposed to oviductal fluid (McNutt et al, 1994). The sperm reservoir within the oviductal isthmus therefore contains a selected and highly fertile subpopulation of the original ejaculate (Ellington et al, 1999;Gualtieri and Talevi, 2003;Suarez, 1998) and it is unsurprising that measures of motility based on whole ejaculates show little correlation with fertility under competitive conditions such as heterospermic insemination.…”

Section: Introductionmentioning

confidence: 99%

Sperm selection and competition in pigs may be mediated by the differential motility activation and suppression of sperm subpopulations within the oviduct

Satake

Elliott

Watson

et al. 2006

Journal of Experimental Biology

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SUMMARY When spermatozoa from two or more boars are mixed and females inseminated,the resulting litters are often skewed in favour of one male but there is currently no satisfactory physiological explanation for this effect. However,to reach the oocytes, the spermatozoa must enter the oviduct where they are exposed to factors that modulate their activity. They either become sequestered within the oviductal sperm reservoir or bypass the reservoir and proceed towards the oocytes. The oviduct may therefore hold the key to mammalian sperm selection, thereby explaining why laboratory tests of sperm function, performed on whole ejaculates, are unable to account for the boar-specific skewing effects. We have previously shown that boar sperm motility is highly stimulated by bicarbonate, a naturally abundant component of oviductal fluid. Using motility-based sperm subpopulation analysis, we show here that the relative sizes of bicarbonate-responsive and unresponsive sperm subpopulations vary between individual boars. Proteins derived from oviduct epithelial plasma membranes suppress the activation response and modify sperm movement trajectories in a subpopulation-specific and dose-dependent manner. The suppression response varies between boars and some spermatozoa remain unsuppressed in the presence of oviductal proteins. When boars are ranked according to their susceptibility to bicarbonate-induced stimulation, rankings differ depending upon the presence or absence of oviductal proteins. The suppression response is not caused by inhibition of bicarbonate uptake; on the contrary this is enhanced by oviductal proteins. We suggest that the boar-specific and sperm subpopulation-specific interactions between sperm motility activation and suppression responses are likely to result in sperm selection before the spermatozoa meet the oocytes.

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Selection of highly fertilization-competent bovine spermatozoa through adhesion to the Fallopian tube epithelium in vitro

Cited by 65 publications

References 18 publications

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

The endocannabinoid system in bull sperm and bovine oviductal epithelium: role of anandamide in sperm–oviduct interaction

Sperm selection and competition in pigs may be mediated by the differential motility activation and suppression of sperm subpopulations within the oviduct

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