Selection of Highly Specific Aptamers by Graphene Oxide-SELEX to Ultrasensitive Label-Free Impedimetric Biosensor Development for Glyphosate Detection

Chergui, Siham; Rhili, Khaled; Abrego-Martínez, J.C.; Jiménez, Gastón Contreras; Siaj, Mohamed

doi:10.1021/acsagscitech.1c00147

Cited by 14 publications

(7 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When SME was added to the solution of the aptamer, the CD spectrum varied differently, with the amplitude reduced, or the characteristic absorption peak of aptamers showed a sighted blueshift. This suggested a transition from a B-form duplex to a hairpin [41], indicating the ability of aptamer sulf-1 and sulf-15 to fold into different conformations induced by target binding [42]. represents the concentration of sulfameter, and y is the ratio of the fluorescence intensity between the experimental group and the control group).…”

Section: Characterization Of Affinity and Specificitymentioning

confidence: 99%

Selected ssDNA aptamers–graphene oxide‐based fluorescent biosensor to detect sulfameter in milk

Zhang

Tian

et al. 2023

Luminescence

View full text Add to dashboard Cite

The abuse of sulfameter (SME) in animal husbandry can cause drug resistance and toxic or allergic reactions in humans. Therefore, it is very important to establish a simple, inexpensive, and efficient method for detecting SME in food. In this work, we propose a single fluorescent aptamer/graphene oxide (GO)‐based biosensor to detect SME residues in milk. Aptamers that specifically bind to SME were screened using capture‐SELEX and a ssDNA library immobilized on magnetic beads. The 68 active candidate aptamers were chemically synthesized for specificity and affinity characterization. Among the aptamers, the aptamer sulf‐1 revealed the highest affinity (Kd = 77 ± 15 nM) to SME and was selected to construct a GO‐based fluorescent biosensor for real milk sample detection. Under optimal conditions, the single fluorescent aptasensor had a wide linear range (R2 was 0.997) from 7 to 336 ng/ml and a low detection limit of 3.35 ng/ml that was calculated with a 3SD/slope. The single fluorescent method was also validated using SME‐fortified milk samples, showing average recoveries ranging from 99.01% to 104.60% with a relative standard deviation of less than 3.88%. These results demonstrate that this novel aptamer sensor provides an opportunity for sensitive, convenient, and accurate detection of SME residues in milk.

show abstract

Section: Characterization Of Affinity and Specificitymentioning

confidence: 99%

Selected ssDNA aptamers–graphene oxide‐based fluorescent biosensor to detect sulfameter in milk

Zhang

Tian

et al. 2023

Luminescence

View full text Add to dashboard Cite

show abstract

“…15,16 Aptamers have been increasingly used as affinity agents to capture analytes in sensors due to their many advantages over antibodies, including stability, tunability of affinity and selectivity, low cost, in vitro synthesis, reproducibility, and ability to be easily chemically modified. 17,18 Aptamer-based sensors have been used for many clinically and environmentally relevant small, charged molecules such as neurotransmitters (e.g., serotonin, 19 dopamine, 20 and histamine 21 ), pesticides (e.g., glyphosate 16 and imidacloprid 22 ), toxins (e.g., ochratoxin A), 23 and drugs (e.g., tenofovir). 24 To develop and improve sensors, it is important to effectively characterize the interactions between affinity agents and their targets of interest using methods such as isothermal titration calorimetry (ITC), fluorescence quenching, or SPR.…”

Section: ■ Introductionmentioning

confidence: 99%

“…While antibodies are the traditional affinity and capture agents used for various sensors like enzyme-linked immunosorbent assays (ELISA) or surface plasmon resonance (SPR), there are not many natural antibodies for small molecules since they are typically not immunogenic . However, newer types of affinity agents have been developed which are much more easily obtained for small molecules, such as aptamers, which are single-stranded oligonucleotides selected for a specific target using systematic evolution of ligands by exponential enrichment (SELEX). , Aptamers have been increasingly used as affinity agents to capture analytes in sensors due to their many advantages over antibodies, including stability, tunability of affinity and selectivity, low cost, in vitro synthesis, reproducibility, and ability to be easily chemically modified. , Aptamer-based sensors have been used for many clinically and environmentally relevant small, charged molecules such as neurotransmitters (e.g., serotonin, dopamine, and histamine), pesticides (e.g., glyphosate and imidacloprid), toxins (e.g., ochratoxin A), and drugs (e.g., tenofovir) …”

Section: Introductionmentioning

confidence: 99%

Investigation of Charged Small Molecule–Aptamer Interactions with Surface Plasmon Resonance

Froehlich

Haynes

2023

Anal. Chem.

View full text Add to dashboard Cite

Investigating the interactions between small, charged molecules and aptamers using surface plasmon resonance (SPR) is limited by the inherent low response of small molecules and difficulties with nonspecific electrostatic interactions between the aptamer, analyte, and sensor surface. However, aptamers are increasingly being used in sensors for small molecule detection in critical areas like healthcare and environmental safety. The ability to probe these interactions through simple, direct SPR assays would be greatly beneficial and allow for the development of improved sensors without the need for complicated signal enhancement. However, these assays are nearly nonexistent in the current literature and are instead surpassed by sandwich or competitive binding techniques, which require additional sample preparation and reagents. In this work, we develop a method to characterize the interaction between the charged small molecule serotonin (176 Da) and an aptamer with SPR using streptavidin−biotin capture and a high-ionic-strength buffer. Additionally, other methods, such as serotonin immobilization and thiol-coupling of the aptamer, were investigated for comparison. These techniques give insight into working with small molecules and allow for quickly adapting a binding affinity assay into a direct SPR sensor.

show abstract

“…The GO-SELEX is a distortion-free selection approach for aptamers incapacitating the limitations of immobilization-based SELEX methods. [19,20] Aptamer-target interaction and its detection at nanomolar scales with minimum signal amplification are prerequisites for the use in a biosensor at the grassroots level. Quartz crystal microbalance (QCM) has been employed for sensitive and labelfree detection of different analytes at low nanomolar scales.…”

Section: Introductionmentioning

confidence: 99%

Selection of Highly Specific DNA Aptamer for the Development of QCM‐Based Arsenic Sensor

Kumar,

Singh,

Verma

2023

ChemBioChem

View full text Add to dashboard Cite

Heavy metal arsenic is a water pollutant that affects millions of lives worldwide. A novel aptamer candidate for specific and sensitive arsenic detection was identified using Graphene Oxide‐SELEX (GO‐SELEX). Eleven rounds of GO‐SELEX were performed to screen As(III) specific sequences. The selected aptamer sequences were evaluated for their binding affinity. The dissociation constant of the best aptamer candidate, As‐06[1], was estimated by fluorescence recovery upon target addition, and it was found to be 8.15 nM. A QCM‐based biosensing platform was designed based on the target‐triggered release of aptamer from the QCM electrode. An rGO‐SWCNT nanocomposite was adsorbed on the gold surface, and the single‐stranded probe was stacked on the rGO‐CNT layer. Upon addition of the target to the solution, a concentration‐dependent release of the ssDNA probe was observed and recorded as the change in the electrode frequency. The developed QCM sensor showed a dynamic linear range from 10 nM to 100 nM and a low detection limit of 8.6 nM. The sensor exhibited excellent selectivity when challenged with common interfering anions and cations. [1] The Provisional IN Patent Application No.: 202311048776, titled An arsenic‐binding aptamer and method of preparing the same, filed on July 20, 2023.

show abstract

Selection of Highly Specific Aptamers by Graphene Oxide-SELEX to Ultrasensitive Label-Free Impedimetric Biosensor Development for Glyphosate Detection

Cited by 14 publications

References 52 publications

Selected ssDNA aptamers–graphene oxide‐based fluorescent biosensor to detect sulfameter in milk

Selected ssDNA aptamers–graphene oxide‐based fluorescent biosensor to detect sulfameter in milk

Investigation of Charged Small Molecule–Aptamer Interactions with Surface Plasmon Resonance

Selection of Highly Specific DNA Aptamer for the Development of QCM‐Based Arsenic Sensor

Contact Info

Product

Resources

About