Selection of Lactiplantibacillus Strains for the Production of Fermented Table Olives

Abstract

Lactiplantibacillus strains (n. 77) were screened for technological properties (e.g., xylose fermentation, EPS production, antimicrobial activity, tolerance to NaCl and phenolic compounds, oleuropein degradation and hydroxytyrosol formation) relevant for the production of fermented table olives. Survival to olive mill wastewater (OMW) and to simulated gastro-intestinal tract (GIT), the capability to grow at different combinations of NaCl and pH values, radical scavenging activities and biofilm formation were further investigated in 15 selected strains. The screening step revealed high diversity among Lactiplantibacillus strains. Most of the strains were able to ferment xylose, while only a few strains produced EPS and had inhibitory activity against Y. lipolytica. Resistance to phenolic compounds (gallic, protocatechuic, hydroxybenzoic and syringic acids), as well as the ability to release hydroxytyrosol from oleuropein, was strain-specific. OMWs impaired the survival of selected strains, while combinations of NaCl ≤ 6% and pH ≥ 4.0 were well tolerated. DPPH and hydroxyl radical degradation were strain-dependent, while the capability to form biofilm was affected by incubation time. Strains were very tolerant to the GIT. The genome of Lpb. pentosus O17 was sequenced and analysed to verify the presence of genes involved in the degradation and metabolism of phenolic compounds. O17 lacks carboxylesterase and gallate decarboxylase (subunits B and D) sequences, and its gene profile differs from that of other publicly available Lpb. pentosus genomes.

In Silico Evidence of the Multifunctional Features of Lactiplantibacillus pentosus LPG1, a Natural Fermenting Agent Isolated from Table Olive Biofilms

“…In these previous studies, this strain also showed high esterase activity, involved in aroma production and degradation of the bitter compound oleuropein. The subsequent in silico analysis confirmed the esterase activity, as the LPG1 strain is predicted to contain genes encoding esterase, carboxylesterase, gallate decarboxylase, p-coumaric acid decarboxylase, and the transcriptional regulator PadR, all of which are involved in the degradation of phenolic compounds [68]. On the contrary, no gene encoding β-glucosidase was predicted, also supporting the previous results that showed the phenotype was not positive for this activity [8].…”

In silico genomic analysis of the potential probiotic Lactiplantibacillus pentosus CF2-10N reveals promising beneficial effects with health promoting properties

“…These strains included L. pentosus IG1 harboring a circular chromosome of 3,687,424 bp (GC content of 44.9%) and 7 plasmids (2.5-125.9 kb; Maldonado-Barragán et al, 2011), L. pentosus strains (IG8, IG9, IG10 and IG11) recovered from biofilms on the skin of green table olives with circular chromosome sizes in the range of 3,787,967 to 3,811,295 bp (GC content of 45.9-45.95%) and 6 to 7 plasmids (Calero-Delgado et al, 2019) and L. pentosus O17 isolated from brines of treated table olives (Cerignola cv.) with a circular chromosome of 3,850,701 bp (GC content of 45.9%; Zotta et al, 2022). This fact indicated their adaptation to a brine-specific lifestyle notably in relation to genes involved in carbohydrate transport and metabolism (307 CDSs and 279 in L. pentosus CF2-10 N and MP-10, respectively) and amino acid metabolism 2017) reported a high level of niche conservatism within the well-supported phylogenetic groups of the genus Lactobacillus (including the recently reclassified genus Lactoplantibacillus), with lifestyles ranging from free-living with large genome size to strictly symbiotic or host adapted with small genome size.…”

Development of a Multiplex PCR Assay for Efficient Detection of Two Potential Probiotic Strains Using Whole Genome-Based Primers

“…pentosus L33 presents a sequence identity of 99.9%, with Lp. pentosus O12, a strain recently isolated from fermented table olives [38] (Figure 2).…”