Selection of suitable reference genes for quantitative real time PCR in different Tulasnella isolates and orchid-fungus symbiotic germination system

Wang, Tao; Cao, Xiaolu; Wang, Xiaojing; Chi, Miao; Li, Lubin; Yao, Na

doi:10.1007/s11033-020-06085-z

Cited by 2 publications

(1 citation statement)

References 41 publications

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“…Those qualities made the bands useful for downstream applications. The fungal genomic DNA amplified by PCR from all of the recovered isolates were positive yielding an amplicon of 500 bp (Figure 4) hence were taken appropriately for Sanger sequencing procedures (Wang et al, 2021). Bioinformatics analysis of the sequenced fungal isolates revealed 9 isolates out of 10 of the consensus sequences had more than 94% nucleotide identity with different species of Fusarium (Table 2).…”

Section: Molecular Characterizationmentioning

confidence: 99%

Occurrence of the entomopathogenic Fungi of Spodoptera frugiperda (Lepidoptera: Noctuidae) in selected areas of Tanzania

Nkuwi,

RWEGASIRA,

CHILAGANE

2023

EAJSTI

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Spodoptera frugiperda (J.E. Smith) is a highly destructive pest that affects major food and cash crops in Sub-Saharan Africa. Maize, in particular, is a preferred host for this pest, posing a significant threat to food security. Initially, conventional synthetic pesticides were widely used to combat the pest. However, the potential of entomopathogenic fungi (EPF) as cost-effective and safe alternative has been recognized. The objective of this study was to collect and identify the local EPF species in selected areas of Tanzania's mainland, namely Mwanza, Morogoro, Coast, and Songwe regions. Morphological and molecular methods were employed to identify the fungal species recovered from 100 S. frugiperda cadavers. The findings revealed that 90% of the recovered fungi belonged to the genera of Fusarium while the remaining 10% were Clonostachys. These results suggest that Fusarium species hold promise as effective bio-control agents against S. frugiperda due to their wide distribution and tolerance to field disturbances. However, additional studies are necessary to validate the effectiveness of these recovered fungi against S. frugiperda.

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Section: Molecular Characterizationmentioning

confidence: 99%

Occurrence of the entomopathogenic Fungi of Spodoptera frugiperda (Lepidoptera: Noctuidae) in selected areas of Tanzania

Nkuwi,

RWEGASIRA,

CHILAGANE

2023

EAJSTI

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Characterization of mycorrhiza associating with Encyclia tampensis and its effect on plant health

Garcia,

Downing,

Khoddamzadeh

2024

Preprint

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Starting in the late 1800’s orchids were heavily poached, leaving many species to reach critically low numbers. Coupled with habitat loss and urbanization many orchid populations were extirpated in southern Florida. Due to lack of endosperm, orchid seeds are reliant on specific mycorrhizal fungi to obtain nutrients to enable embryo development resulting in very low germination rates in nature. This obligatory relationship with mycorrhizal fungi complicates orchid re-establishment. The research project aims are (1) to evaluate the correlation between phenotypic traits and optimal growing conditions in various micro-climate conditions, and (2) to assess the range of mycorrhizal diversity in urban and botanic garden settings. The native orchid, Encyclia tampensis, was sampled from naturally occurring and lab propagated orchids at Fairchild Tropical Botanic Garden (FTBG), Naples Botanical Garden (NBG), and Downtown Doral Park (DDP) on oak, cypress, and palm trees. Phenotypic measurements (chlorophyll content, light intensity, height on the tree, number of leaves, root and shoot lengths) and root sampling (fungal isolations, DNA identification) were conducted. Increased SPAD and NDVI values measuring chlorophyll content as a plant health parameter were observed at FTBG, while DDP and NBG had comparable values indicating the suitability of botanic gardens and urban spaces as reintroduction sites for orchid. Across all 3 sites and host trees, two distinct clades of mycorrhizal fungi within the genus Tulasnella were recovered. The results of this study will help maximize current conservation and reintroduction programs within developed urban areas and botanical gardens for best management practices on orchids.

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Selection of suitable reference genes for quantitative real time PCR in different Tulasnella isolates and orchid-fungus symbiotic germination system

Cited by 2 publications

References 41 publications

Occurrence of the entomopathogenic Fungi of Spodoptera frugiperda (Lepidoptera: Noctuidae) in selected areas of Tanzania

Occurrence of the entomopathogenic Fungi of Spodoptera frugiperda (Lepidoptera: Noctuidae) in selected areas of Tanzania

Characterization of mycorrhiza associating with Encyclia tampensis and its effect on plant health

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