Selection of uremic toxin-reducing probiotics in vitro and in vivo

Fang, Chen-Yu; Lu, Je-Ruei; Chen, Bao-Ji; Wu, Chieh Hsi; Chen, Yen‐Po; Chen, Ming‐Ju

doi:10.1016/j.jff.2014.01.018

Cited by 16 publications

(15 citation statements)

References 33 publications

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“…LAB screen for indoxyl sulfate (IS) inhibitory activity. LAB strains were screened for their ability to reduce IS levels as previously described (23). The strains were incubated in MRS broth with 60 µg/ml IS and the one with the highest inhibitory activity was selected.…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus salivarius BP121 prevents cisplatin‑induced acute kidney injury by inhibition of uremic toxins such as indoxyl sulfate and p‑cresol sulfate via alleviating dysbiosis

Lee¹,

Park

Kim³

et al. 2020

Int J Mol Med

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The gut microbiota is important for maintaining the integrity of the intestinal barrier, promoting immunological tolerance and carrying out metabolic activities that have not evolved in hosts. Intestinal dysbiosis is associated with chronic kidney disease and probiotic supplementation has been shown to be beneficial. However, it is not known whether gut microorganisms-specifically, lactic acid bacteria (LAB) can protect against acute kidney injury (AKI). To address this issue, the present study investigated the effects of Lactobacillus salivarius BP121, an intestinal LAB isolated from the feces of newborns, in a rat model of cisplatin-induced AKI and also in Caco-2 human intestinal epithelial cells. BP121 prevented cisplatin-induced AKI in rats, as demonstrated by decreases in inflammation and oxidative stress in kidney tissue and in serum levels of uremic toxins such as indoxyl sulfate (IS) and p-cresol sulfate (PCS). BP121 also reduced intestinal permeability, as determined using fluorescein isothiocyanate-dextran by immunohistochemical detection of tight junction (TJ) proteins such as zona occludens-1 and occludin. The abundance of Lactobacillus spp., which are beneficial intestinal flora, was increased by BP121; this was accompanied by an increase in the concentrations of short-chain fatty acids in feces. Additionally, H 2 O 2 -induced TJ protein damage was reduced in Caco-2 cells treated with BP121 culture supernatant, an effect that was reversed by the 5' AMP-activated protein kinase (AMPK) inhibitor Compound C and Toll-like receptor (TLR)4 inhibitor TLR4-IN-C34. In conclusion, this study demonstrated that L. salivarius BP121 protects against cisplatin-induced AKI by decreasing inflammation and oxidative stress and this renoprotective effect is partially mediated by modulating the gut environment and thereby suppressing IS and PCS production as well as by regulating AMPK and TLR4 dependent TJ assembly.

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Section: Methodsmentioning

confidence: 99%

Lactobacillus salivarius BP121 prevents cisplatin‑induced acute kidney injury by inhibition of uremic toxins such as indoxyl sulfate and p‑cresol sulfate via alleviating dysbiosis

Lee¹,

Park

Kim³

et al. 2020

Int J Mol Med

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“…Second, the two selected conventional probiotic hosts, L. delbrueckii and L. gasseri (our laboratory stock), were isolated from yogurt and human milk, respectively, revealing that the two strains originate from natural and safe sources. Finally, L. delbrueckii has been shown previously to display both antibacterial activities and detoxification capacity against uremic toxins in vitro [ 28 , 40 ]. As for L. gasseri (our laboratory stock), we confirmed that the milk-isolated L. gasseri can display anti-bacterial activities, as well as both bile salt and acid tolerance abilities in our preliminary analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, it will be interesting to test the antibacterial potency of our recombinant L. delbrueckii/HLF and L. delbrueckii/BLF clones against the growth of G. vaginalis in our next study. Furthermore, as for the beneficial effects of conventional L. delbrueckii , this strain has also been shown to reduce uremic toxin levels, mainly those of indoxyl sulfate, in vitro [ 40 ]. Therefore, we believe that the L. delbrueckii /BLF and L. delbrueckii/ HLF strains engineered in our study may possess the beneficial characteristics of both conventional strains and biological activities of LFs, and it will be intriguing to observe whether the detoxification efficiency of recombinant L. delbrueckii /BLF and L. delbrueckii/ HLF against uremic toxins can be confirmed in a future study.…”

Section: Resultsmentioning

confidence: 99%

A Single Plasmid of Nisin-Controlled Bovine and Human Lactoferrin Expressing Elevated Antibacterial Activity of Lactoferrin-Resistant Probiotic Strains

et al. 2021

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Lactoferrin (LF) is a multifunctional protein found in mammals, and it shows broad-spectrum antimicrobial activity. To improve the functional properties of specific probiotics in order to provide both the beneficial characteristics of lactic acid bacteria and the biological activity of LF, cDNAs of bovine LF (BLF), human LF (HLF), or porcine LF (PLF) were cloned into a nisin-inducible plasmid. These were then transformed into the selected eight probiotics, which are LF-resistant hosts. Expression of recombinant LFs (rLFs) was analyzed via SDS-PAGE and Western blot analysis. Although the selected host strains may not contain the nisRK genes (NisK, the sensor kinase; NisR, the regulator protein), the components of autoregulation, a low level of LFs expression can be successfully induced by using nisin within bacterial cells in a time-dependent manner in three engineered clones, including Lactobacillus delbrueckii/HLF, L. delbrueckii/BLF, and L. gasseri/BLF. Lactobacillus delbrueckii and Lactobacillus gasseri originate from yogurt and human milk, respectively, and both strains are functional probiotic strains. Therefore, we further compared the antibacterial activities of disrupted recombinant probiotic clones, conventional strains (host control), and vector control ones by using agar diffusion and broth inhibition analysis, and the expression of rLFs in the above three clones considerately improved their antibacterial efficacies against four important food-borne pathogens, namely, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, and Salmonellaenterica. In conclusion, this study provides a simple strategy for the production of functional LFs (BLF and HLF) in both functional and LF-resistant hosts for applications in the field.

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“…A recent report suggested that LAB strains are important functional food ingredients to alleviate allergic symptoms (Takeda et al, 2014). Several evidence confirmed the application of potential probiotic LAB resulted in the prevention of gastrointestinal diseases, type-2 diabetes, and ability to degrade luminal oxalate in the gut Fang et al, 2014;Gill & Guarner, 2004). Hyperoxaluria is an elevated urinary oxalate excretion (Asplin, 2002), resulting from high dietary oxalate intake, intestinal oxalate absorption, fat malabsorption, variations in the intestinal oxalate degrading bacteria and genetic modification of oxalate transporters (Robijn, Hoppe, Vervaet, D'Haese, & Verhulst, 2011).…”

Section: Introductionmentioning

confidence: 99%

Oral administration of indigenous oxalate degrading lactic acid bacteria and quercetin prevents calcium oxalate stone formation in rats fed with oxalate rich diet

Gomathi

Ponnusamy

Anbazhagan

et al. 2015

Journal of Functional Foods

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Selection of uremic toxin-reducing probiotics in vitro and in vivo

Cited by 16 publications

References 33 publications

Lactobacillus salivarius BP121 prevents cisplatin‑induced acute kidney injury by inhibition of uremic toxins such as indoxyl sulfate and p‑cresol sulfate via alleviating dysbiosis

Lactobacillus salivarius BP121 prevents cisplatin‑induced acute kidney injury by inhibition of uremic toxins such as indoxyl sulfate and p‑cresol sulfate via alleviating dysbiosis

A Single Plasmid of Nisin-Controlled Bovine and Human Lactoferrin Expressing Elevated Antibacterial Activity of Lactoferrin-Resistant Probiotic Strains

Oral administration of indigenous oxalate degrading lactic acid bacteria and quercetin prevents calcium oxalate stone formation in rats fed with oxalate rich diet

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