Selection Technologies and Applications of Nucleic Acid Aptamers

Yoshimoto, Keitaro

doi:10.2116/analsci.highlights1910

Cited by 7 publications

(8 citation statements)

References 19 publications

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“…Nucleic acid aptamers, which are single‐stranded DNA (ssDNA) and RNA with high affinity to specific targets such as proteins, could be powerful anticoagulants. Compared with antibodies, DNA aptamers possess many advantages such as relatively small size, greater stability, potential for chemical modification, lower toxicity, and reduced immunogenicity 1 . Aptamers have been studied as smart biomolecules in numerous investigations for diagnostic 2 and therapeutic applications 3 .…”

Section: Introductionmentioning

confidence: 99%

“…Compared with antibodies, DNA aptamers possess many advantages such as relatively small size, greater stability, potential for chemical modification, lower toxicity, and reduced immunogenicity. 1 Aptamers have been studied as smart biomolecules in numerous investigations for diagnostic 2 and therapeutic applications. 3 Recently, we developed the systematic evolution of ligands by exponential enrichment (SELEX) with microbead-assisted capillary electrophoresis (MACE), referred to as MACE-SELEX, which features a sophisticated separation step with high sensitivity based on capillary electrophoresis separation using target-coupled microbeads.…”

Section: Introductionmentioning

confidence: 99%

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Design strategy of antidote sequence for bivalent aptamer: Rapid neutralization of high‐anticoagulant thrombin‐binding bivalent DNA aptamer‐linked M08 with HD22

Yoshitomi

Wakui

Miyakawa

et al. 2021

Research and Practice in Thrombosis and Haemostasis

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Background Bivalent thrombin‐binding aptamers (TBAs) have great potential for the treatment of thrombosis because they exhibit high anticoagulant activity, and their complementary single‐stranded DNA (ssDNA) sequences work as an antidote. However, a design strategy for antidote sequences against bivalent aptamers has not been established. Objectives To develop bivalent TBAs using M08, which exhibits higher anticoagulant activity than the previously reported exosite Ⅰ–binding DNA aptamers, such as HD1, an exosite Ⅱ–binding DNA aptamer (HD22) was linked to M08 with various types of linkers. In addition, short‐length complementary ssDNAs were designed to neutralize the optimized bivalent aptamer effectively and rapidly. Results Among the bivalent aptamers of M08 linked to HD22 with various types of linkers, M08‐T15‐HD22 possessed approximately 5‐fold higher anticoagulant activity than previously reported bivalent aptamers. To neutralize the activity of the 87‐meric M08‐T15‐HD22, complementary ssDNA sequences with different lengths and hybridization segments were designed. The complementary sequence against the M08 moiety played a more important role in neutralizing than that against the HD22 moiety. Hybridization of the T15 linker in the M08‐T15‐HD22 with the A15 sequence in the antidote accelerated neutralization due to toehold‐mediated strand displacement. Interestingly, some shorter‐length antidotes showed higher neutralizing activity than the full complementary 87‐meric antidote, and the shortest, 34‐meric antidote, neutralized most effectively. Conclusions A pair comprising an 87‐meric bivalent TBA containing M08 and a 34‐meric short‐length antidote with high anticoagulant and rapid neutralizing activities was developed. This design strategy of the DNA sequence can be used for other bivalent DNA aptamers and their antidotes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Design strategy of antidote sequence for bivalent aptamer: Rapid neutralization of high‐anticoagulant thrombin‐binding bivalent DNA aptamer‐linked M08 with HD22

Yoshitomi

Wakui

Miyakawa

et al. 2021

Research and Practice in Thrombosis and Haemostasis

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“…For example, nucleic acid aptamers are small sequences of DNA or RNA that fold into well-defined and stable 3D sequence-dependent structures. This inherent feature enables them to efficiently interact with molecular targets, ranging from metal ions and small organic compounds to large protein targets or even complex molecules [35,36]. Aptamers are successfully generated from combinatorial nucleic acid libraries using in vitro selection methods, which are usually more cost-effective than antibody production and purification.…”

Section: Introductionmentioning

confidence: 99%

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Chang

2021

Biosensors

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Surface plasmon resonance (SPR) can track molecular interactions in real time, and is a powerful as well as widely used biological and chemical sensing technique. Among the different SPR-based sensing applications, aptamer-based SPR biosensors have attracted significant attention because of their simplicity, feasibility, and low cost for target detection. Continuous developments in SPR aptasensing research have led to the emergence of abundant technical and design concepts. To understand the recent advances in SPR for biosensing, this paper reviews SPR-based research from the last seven years based on different sensing-type strategies and sub-directions. The characteristics of various SPR-based applications are introduced. We hope that this review will guide the development of SPR aptamer sensors for healthcare.

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“…Compared with antibodies, aptamers have several significant advantages: (a) they have high chemical stability and are insensitive to the change in pH value, and can be stored at ambient temperature for a long time; (b) the denaturation of aptamers is highly reversible, that is to say, aptamers can be denatured at high temperature and renatured at low temperature; (c) they are easy to obtain, because they can be screened nonbiologically in vitro, and can be synthesized with high purity when determining the sequence; (d) they can recognize many kinds of targets, including proteins, nucleic acids, cells and various small-molecule compounds; (e) they have high affinity to targets with dissociation constants in the range of 10 −6 -10 −12 M; and (f) they are easy to modify and are suitable for binding with nanomaterials. In view of this, aptamers have been widely used in biomedical, analytical and other fields (Dunn et al, 2017;Groff et al, 2015;Hamula et al, 2011;Kong & Byun, 2013;Nevídalová et al, 2020;Song et al, 2019;Tombelli et al, 2007;Yoshimoto, 2019).…”

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confidence: 99%

Capillary electrophoresis‐based methodology for screening of oligonucleotide aptamers

Guo

Chen

2021

Biomedical Chromatography

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As a new molecular recognition element, oligonucleotide aptamer not only has higher affinity and specificity to target molecules, but also has the advantages of wide recognition range, in vitro synthesis and chemical stability compared with conventional antibodies. Since a kind of screening method termed systematic evolution of ligands by exponential enrichment (SELEX) was reported, scientists have extensively researched the methodology of how to highly and efficiently screen out aptamers from a library consisting of a large number of random oligonucleotides. Certainly capillary electrophoresis-based screening methodologies, including nonequilibrium capillary electrophoresis of equilibrium mixtures, equilibrium capillary electrophoresis of equilibrium mixtures, non-SELEX, ideal-filter capillary electrophoresis, capillary transient isotachophoresis, etc., are revolutionary. Compared with conventional SELEX, these capillary electrophoresis-based methodologies show incomparable advantages such as the single-round screening of aptamers and increased successful screening rate. Methodology studies on the screening process of aptamers are comprehensively reviewed.

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Selection Technologies and Applications of Nucleic Acid Aptamers

Cited by 7 publications

References 19 publications

Design strategy of antidote sequence for bivalent aptamer: Rapid neutralization of high‐anticoagulant thrombin‐binding bivalent DNA aptamer‐linked M08 with HD22

Design strategy of antidote sequence for bivalent aptamer: Rapid neutralization of high‐anticoagulant thrombin‐binding bivalent DNA aptamer‐linked M08 with HD22

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Capillary electrophoresis‐based methodology for screening of oligonucleotide aptamers

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