Selective adsorption of protein by a high-efficiency Cu²⁺-cooperated magnetic imprinted nanomaterial

Abstract

We report a core-shell magnetic molecularly imprinted polymer with high affinity through a facile sol-gel method for the selective adsorption of bovine hemoglobin from real bovine blood. Copper ions grafted on the surface of the matrix could immobilize template protein through chelation, which greatly enhances the orderliness of imprinted cavities and affinity of polymers. The obtained products exhibit a desired level of magnetic susceptibility, resulting in the highly efficient adsorption process. The results of adsorption experiments show that the saturation adsorption capacity of imprinted products could reach 116.3 mg/g within 30 min. Meanwhile, the specific binding experiment demonstrates the high selectivity of polymers for bovine hemoglobin. Furthermore, satisfactory reusability is demonstrated by ten adsorption-desorption cycles with no obvious deterioration in binding capacity. Electrophoretic analysis suggests the polymer could be used successfully in separation and enrichment of bovine hemoglobin from the bovine blood sample, which exhibits potential application in pretreatment of proteomics.

A study on the preparation and application of a core–shell surface imprinted uranyl magnetic chelating adsorbent

“…9B strongly magnetic and allowed for use as an effective magnetic separation carrier. [43][44][45] UMCA particles could be dispersed uniformly in the solution (Fig. 9A).…”

Magnetic molecularly imprinted polymer based on dynamically established metal‐mediated binding sites for the determination of trace captopril in rat plasma

“…MIP was prepared by the sol-gel method [26]. In brief, 22 mg of CAP (0.1 mmol) was dissolved in 18 ml of ethanol.…”

Fabrication of Metal Ion–BSA Chelation-Targeted Surface-Imprinted Particles for Protein Separation

“…Li et al 43 proposed a new strategy to selectively recognize and functionalize magnetic CNTs by sol−gel polymerization using Cu 2+ -mediated interaction, and it exhibited a high adsorption capacity (13.35 μg/mg) in 40 s, a good imprinting factor (IF) of 3.41, and a high magnetic responsivity (44.38 emu/g), which can be used as an ideal adsorbent for rapid isolation and enrichment of target analytes. Shi et al 44 obtained imprinted shells with specific recognition cavities for BHb using Fe 3 O 4 nanoparticles with surface carboxyl-chelated Cu(II) to anchor the BHb molecule; the imprinted polymer layer was formed by the condensation of 3-aminopropyltriethoxysilane and octyltrimethoxysilane; the imprinted polymer exhibited high adsorption capacity and selectivity with the help of Cu(II). Guo et al prepared Cu(II)-chelated MIPs (MG@Cu 2+ @ MIPs) for the specific identification of ovalbumin (OVA) from egg white samples.…”