Selective and Specific Adsorbents for Medical Therapy

Immobilization of DNA onto a polymer support and its potentiality as immunoadsorbent

“…Similar to reaction code I, the epoxide is expected to react with the amino group in the base of DNA through reaction code IV. Because the prim-amine in the base is substituted to a pyrimidine ring, the lone pair of electrons is likely not to be localized but pushed out to the ring and 40% to 50% of the C-N bonds have the electronic property characteristic to double bond (Blackburn, 1990). Therefore, it is reasonable to assume that the amino group carried by the base has low nucleophilicity.…”

“…Any release of immobilized DNA from the adsorbent into the aqueous phase during the adsorption process should be avoided, especially when the adsorbent is used for extracorporeal blood detoxification (Behm et al, 1989). It was found from Figure 7 that the DNA immobilized through polyion complexation was more stable to serum exposure than the covalently bound DNA.…”

“…The immobilization of DNA to polymer surfaces provides convenient tools in a variety of fields including biotechnology and medicine. One of the most promising applications of the technique is protein adsorbents for blood detoxification through extracorporeal circulation of the blood or plasma of patients (Behm et al, 1989). Twenty years ago, selective removal of anti-DNA antibody by extracorporeal immunoadsorption was first reported for the therapy of systemic lupus erythematosus (SLE) using a DNA-cellulose column in which DNA was physically adsorbed to cellulose powders as an affinity ligand for anti-DNA antibody (Terman et al, * To whom all correspondence should be addressed.…”

Biosensors for detection of Low-Density Lipoprotein and its modified forms

“…Experimental results showed that this system was capable to predict the adsorption capacities and concentrations at ng/l. Many researchers have been involved in development of different kinds of adsorbents including non-specific, selective adsorbents and immuno-adsorbent according to the molecular structure of LDL (Behm et al, 1989;Bosch et al, 1997). Piezoelectric technique can be associated to SPR technique to obtain new immunosensor devices.…”

Bioactive Bead Type Cellulosic Adsorbent for Blood Purification

“…Sorbent-perfusion is a novel approach of blood purification which can specifically remove endogenous and exogenous pathogenic toxins from the blood of patients [1]. The technique involves passing whole blood or plasma of the patient through a cartridge filled with an adsorbent which can easily adsorb the toxin molecules, see Figure 1 a,b.…”