Selective anti-cancer activity against melanoma cells using 3-O-acetyl-β-boswellic acid-loaded 3D-Printed scaffold

Abstract

This study aimed to develop a local 3D-printed bioactive graft using poly-caprolacton (PCL) as the drug carrier and 3-O-acetyl-β-boswellic acid (β-ABA) as an anticancer compound. β-ABAloaded 3D-printed scaffold was fabricated and physically characterized. The results indicated more desirable mechanical and physical properties of the β-ABA-loaded PCL mat in comparison with the PCL scaffold. Following sustained release of β-ABA, the β-ABA-loaded PCL scaffold revealed selective cytotoxic activity against melanoma cells, while the PCL+ABA with the bolus delivery of β-ABA was toxic against fibroblast cells. Followed by the induction of apoptosis in melanoma cells at the gene level, the result of the western blot showed that the β-ABA-loaded scaffold significantly up-regulated P53 and down-regulated BCL2, with an increment in the ratio of Bax/BCL2. The selective anti-cancer properties of β-ABA-loaded 3D printed scaffold against melanoma cells indicated that this scaffold could be potentially used as a bioactive graft to improve the melanoma treatment.

Avastin‐Loaded 3D‐Printed Alginate Scaffold as an Effective Antiadhesive Barrier to Prevent Postsurgical Adhesion Bands Formation

“…The concentration of Ava in the released samples was determined by the method reported by Fatemeh et al. [ 29 ] Three sets of samples were examined. The equation below was used to determine the total amount of Ava (Mi) released at a particular time I $$$$\begin{equation}{\mathrm{Mi\ }} = {\mathrm{\ CiV}} + {\mathrm{\Sigma \ C}}\left( {{\mathrm{i}} - 1} \right){\mathrm{Vs}}\end{equation}$$$$i: time; Ci: the concentration of Ava in the released solution; V: the total volume of the released solution; Vs: the volume of the sample.…”

“…At different time points, 0.5 mL of released medium was collected for further analysis and substituted with 0.5 mL of fresh PBS. The concentration of Ava in the released samples was determined by the method reported by Fatemeh et al [29] Three sets of samples were examined. The equation below was used to determine the total amount of Ava (Mi) released at a particular time I…”