Selective arteriography of the spinal cord in posttraumatic paraplegia

Abstract

Subscriptionshttp://jimmunol.org/subscriptions is online at: ImmunologyThe Journal of Information about subscribing to PermissionsPreviously reported experiments (1, 2, 3) on anaphylaxis apparently due to the toxin or toxoid antigen were made upon actively sensitized (antitoxic immune) animals. The results had indicated that while other diphtheria antigens participated to some extent, the major part of the anaphylaxis could be assigned to the toxin or toxoid antigen, provided one used both for the immunization and for the intravenous test minimal amounts of unheated filtrate in which toxin or toxoid was the dominant antigen, and selected for intravenous test the individual guinea pigs that had been "earliest" in antitoxin production. Passive sensitization possesses certain advantages over active sensitization in the distinction of reactions due to toxin-antitoxin from reactions due to other diphtheria antigen-antibody combinations, for with the passive method it is possible to examine each of the sensitizing sera for antibodies other than antitoxin and, when desired, to remove the other antibodies by absorption of the antisera before using them for sensitization. This investigation consisted essentially of a comparison of the anaphylaxis obtained by sensitization with antitoxic sera and that obtained with antibacterial sera. Four different materials were used in the shock tests: toxin-containing broth culture filtrates, solutions of semipurified toxin precipitated from filtrate, solutions of nucleoprotein obtained by alkaline extraction of the bacilli, and solutions of material (principally carbohydrate) extracted by boiling acidified (HC1) suspensions of the bacflli. The primary interest is in the anaphylaxis referable to toxin antigen and antitoxin antibody, the other diphtheria antigens and antibodies being introduced as controls in order to show that 131 A nti-P precipitins. The solutions of nucleoprotein used in these tests were prepared in essentially the same manner as used at Upstate Medical University Health Sciences Library on August 24, 2015 http://www.jimmunol.org/ Downloaded from ANAPHYLAXIS TO DIPHTHEtLIA TOXIN * Although a considerable number of tests were made with different antisera, all of the results reported in this paper were obtained with the same pooled lots of