Selective Binding of Genomic <i>Escherichia coli</i> DNA with ZnO Leads to White Light Emission: A New Aspect of Nano–Bio Interaction and Interface

The development of nanomaterial-based hybrid systems for healthcare and energy-related materials has attracted significant attention nowadays. Here, we have designed a nanocomposite of ZnO nanoparticles (NPs) with anticancer therapeutic drug 9-aminoacridine hydrochloride hydrate (9AA-HCl) for antibacterial and photocatalytic activities. Spectroscopic studies reveal that the photoinduced electron transfer from photoexcited 9AA-HCl to the conduction band of ZnO NP causes the generation of the reactive oxygen species (ROS), which is responsible for antibacterial activity and photocatalytic properties. It is seen that the efficiency of photodegradation of dye molecules increases in ZnO–9AA-HCl nanoconjugated systems than pure ZnO nanoparticles because of efficient charge separation. In addition, the antibacterial efficacy of the nanoconjugate is investigated using a strain of Gram-negative bacteria where the cell-killing activities are observed 99.99 and 100% for 20 and 21 μL/mL nanoconjugate, respectively, and very little cell-killing activity is observed for free ZnO NPs and free drug. Moreover, it is also observed that the nanoconjugate generates sufficient intracellular ROS that can hydrolyze 2′,7′-dichlorodihydrofluoresceindiacetate (DCFH-DA) to highly fluorescent 2′,7′-dichlorofluorescein (DCF). The outcome of the study will provide valuable information for designing new-edge nanoconjugate materials for potential applications in photocatalytic and antibacterial activities.

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“…The shifting of band position of −OH stretching suggests the formation of hydrogen bonding between ZnO NP and 9AA-HCl. 52 , 53 …”

Section: Resultsmentioning

confidence: 99%

Antibacterial and Photocatalytic Properties of ZnO–9-Aminoacridine Hydrochloride Hydrate Drug Nanoconjugates

et al. 2018

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“…The interaction between engineered nanoparticles (NPs) and biomolecules has led to the development of new type of biosensors 1 , 2 and biomolecular targets. 3 , 4 To explore the fundamental 5 , 6 and technological 7 aspects of nano-bio-interaction, a broad range of inorganic, 8 , 9 organic, 10 and hybrid nanostructures 11 or nanocomposites 12 , 13 exhibiting well-defined structural, optical, electrical, and magnetic properties have been targeted by many investigators. Among these, metal–semiconductor nanocomposites possessing synergy between different components are also being projected as a new class of materials for this purpose.…”

Section: Introductionmentioning

confidence: 99%

“…In one of our recent studies, the interaction of fluorescent ZnO rods with DNA, which incidentally resulted in Escherichia coli (EC) DNA-specific interaction leading to white light emission, has been reported. 9 Inspired by this result and motivated by the affinity of gold nanocluster toward oligonucleotides, 38 − 42 we have extended our research with fluorescent nanocomposites to understand the synergistic effect of two fluorescent materials in a single nanocomposite to find out if there could be base-pair-specific interaction with such composites. For this purpose, we have chosen two individual fluorescent materials, ZnO nanoparticles, exhibiting emission at 389 and 513 nm, and gold nanoclusters, exhibiting emission at 610 nm.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent ZnO–Au Nanocomposite as a Probe for Elucidating Specificity in DNA Interaction

et al. 2018

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In this work, we report the interaction of a fluorescent ZnO–Au nanocomposite with deoxyribonucleic acid (DNA), leading to AT-specific DNA interaction, which is hitherto not known. For this study, three natural double-stranded (ds) DNAs having different AT:GC compositions were chosen and a ZnO–Au nanocomposite has been synthesized by anchoring a glutathione-protected gold nanocluster on the surface of egg-shell-membrane (ESM)-based ZnO nanoparticles. The ESM-based bare ZnO nanoparticles did not show any selective interaction toward DNA, whereas intrinsic fluorescence of the ZnO–Au nanocomposite shows an appreciable blue shift (Δλmax = 18 nm) in the luminescence wavelength of 520 nm in the presence of ds calf thymus (CT) DNA over other studied DNAs. In addition, the interaction of the nanocomposite through fluorescence studies with single-stranded (ss) CT DNA, synthetic polynucleotides, and nucleobases/nucleotides (adenine, thymine, deoxythymidine monophosphate, deoxyadenosine monophosphate) was also undertaken to delineate the specificity in interaction. A minor blue shift (Δλmax = 5 nm) in the emission wavelength at 520 nm was observed for single-stranded CT DNA, suggesting the proficiency of the nanocomposite for discriminating ss and ds CT DNA. More importantly, fluorescence signals from the nano-bio-interaction could be measured directly without any modification of the target, which is the foremost advantage emanated from this study compared with other previous reports. The AT base-pair-induced enhancement was also found to be highest for the melting temperature of CT DNA (ΔTmCT = 6.7 °C). Furthermore, spectropolarimetric experiments followed by calorimetric analysis provided evidence for specificity in AT-rich DNA interaction. This study would lead to establish the fluorescent ZnO–Au nanocomposite as a probe for nanomaterial-based DNA-binding study, featuring its specific interaction toward AT-rich DNA.

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“…Upon excitation at4 10 nm, the emission spectrum displays peaks at 485 and 640 nm with similarintensities coveringthe entire visible region (Figure 3a,ii).T he Commission Internationaled e l'Eclairage (CIE) coordinates of white-light emission were found to be x = 0.33, y = 0.36, which is close to that of the pure white (0.33, 0.33) light (Figure 3b). The quantum yield of WLE aqueous micellar solution was found to be as high as 37:3 AE 1:5% ( Figure S13) whichiscomparable or higher than that of the notable all-organic and organic-inorganic hybrid WLE assemblies in water reported to date such as, DP/DCM (36 %), [53] TPDC-Bzbased nanoparticles (35 %) ZnO-ECD NA (34 %), [54] [APR6G][FL] nanoparticles (26 %), [36] TPECVs@RhB (21 %), [55] C-dot-C153-AuNC (19 %), [56] andQ [8]-G1 (12 %) [37] (Tables S5 and S6).…”

Section: White-light Emitting( Wle) Micellesmentioning

confidence: 82%

Tuning the Förster Resonance Energy Transfer through a Self‐Assembly Approach for Efficient White‐Light Emission in an Aqueous Medium

Pallavi

Ahir

et al. 2017

Chemistry A European J

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A simple and cost-effective methodology employing environmentally benign substances for the fabrication of white-light emitting materials is important for practical applications in the field of lighting and display devices. Designing purely organic-based white-light-emitting systems with high quantum efficiency in aqueous media is an unmet challenge. With this objective, a new class of pyridoindole-based hydrophobic fluorophore 6,7,8,9-tetrapropylpyrido[1,2-a]indole-10-carbaldehye (TPIC) was introduced. A strategy of self-assembly using nonionic surfactants was employed to enhance the fluorescence of TPIC in an aqueous medium and was exploited as energy donor. The steady-state and time-resolved emission spectra analysis revealed the micelle-mediated energy transfer from TPIC to Nile red (energy acceptor) leading to tunable fluorescence along with white-light emission. The white-light emitting aqueous solution was obtained with the Commission Internationale de l'Eclairage (CIE) chromaticity coordinates of (0.33, 0.36) and significantly high quantum yield of 37 %. Solid-state white-light emission was achieved retaining the assembly of fluorophores in the form of a gel having the high quantum efficiency of 33 % with CIE coordinates of (0.32, 0.36); close to that of pure white light. The bright white luminescence of the inscription prepared using white-light emitting gel on a solid substrate offers promising applications for full-color flat panel displays.

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Selective Binding of Genomic Escherichia coli DNA with ZnO Leads to White Light Emission: A New Aspect of Nano–Bio Interaction and Interface

Cited by 21 publications

References 71 publications

Antibacterial and Photocatalytic Properties of ZnO–9-Aminoacridine Hydrochloride Hydrate Drug Nanoconjugates

Antibacterial and Photocatalytic Properties of ZnO–9-Aminoacridine Hydrochloride Hydrate Drug Nanoconjugates

Fluorescent ZnO–Au Nanocomposite as a Probe for Elucidating Specificity in DNA Interaction

Tuning the Förster Resonance Energy Transfer through a Self‐Assembly Approach for Efficient White‐Light Emission in an Aqueous Medium

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