Selective Cytotoxicity Evaluation in Anticancer Drug Screening of Fractionated Plant Extracts

Lindholm, Petra; Gullbo, Joachim; Claeson, Per; Göransson, Ulf; Johansson, Senia; Backlund, Anders; Larsson, Rolf; Bohlin, Lars

doi:10.1089/108705702320351187

Cited by 36 publications

(55 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cycloviolacin O2 is a small cyclic cystine‐rich protein isolated from the sweet violet ( Viola odorata L.), which has been shown to exert significant structure‐dependent cytotoxicity to human cancer cell lines in vitro 1, 2. The protein consists of 30 amino acids and is a member of the cyclotide family, a group of genetically encoded plant proteins with a continuous cyclic backbone 3, 4.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of toxicity and antitumor activity of cycloviolacin O2 in mice

et al. 2010

Self Cite

View full text Add to dashboard Cite

Cycloviolacin O2 is a small cyclic cysteine-rich protein belonging to the group of plant proteins called cyclotides. This cyclotide has been previously shown to exert cytotoxic activity against a variety of human tumor cell lines as well as primary cultures of human tumor cells in vitro. This study is the first evaluation of its tolerability and antitumor activity in vivo. Maximal-tolerated doses were estimated to 1.5 mg/kg for single intravenous (i.v.) dosing and 0.5 mg/kg for daily repeated dosing, respectively. Two different in vivo methods were used: the hollow fiber method with single dosing (i.v., 1.0 mg/kg) and traditional xenografts with repeated dosing over 2 weeks (i.v., 0.5 mg/kg daily, 5 days a week). The human tumor cell lines used displayed dose-dependent in vitro sensitivity (including growth in hollow fibers to confirm passage of cycloviolacin O2 through the polyvinylidene fluoride fibers), with IC5o values in the micromolar range. Despite this sensitivity in vitro, no significant antitumor effects were detected in vivo, neither with single dosing in the hollow fiber method nor with repeated dosing in xenografts. In summary, the results indicate that antitumor effects are minor or absent at tolerable (sublethal) doses, and cycloviolacin O2 has a very abrupt in vivo toxicity profile, with lethality after single injection at 2 mg/kg, but no signs of discomfort to the animals at 1.5 mg/kg. Repeated dosing of 1 mg/kg gave a local-inflammatory reaction at the site of injection after 2-3 days; lower doses were without complications.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Cycloviolacin O2 has been subject to extensive cytotoxicity testing in vitro, with promising results that warranted further studies 1, 2, 8. In a panel of 10 human cancer cell lines, cycloviolacin O2 showed cytotoxicity to all the cell lines at concentrations in the low‐micromolar range.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of toxicity and antitumor activity of cycloviolacin O2 in mice

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…The chronic oral toxicity study was done according to OECD guideline 423. [26–27] In this experiment, two groups of Wistar rats ( n = 6) were used. Animals were kept on fast overnight with water ad libitum and food was withheld for 3–4 h after the oral administration of the extracts.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and anticancer evaluation of Scindapsus officinalis (Roxb.) Schott fruits

Shivhare¹,

Patidar²,

Malviya³

et al. 2011

AYU

View full text Add to dashboard Cite

Several methods exist for the treatment of cancer in modern medicine. These include chemotherapy, radiotherapy, and surgery; most cancer chemotherapeutants severely affect the host normal cells. Hence the use of natural products now has been contemplated of exceptional value in the control of cancer. Plant-derived natural products such as flavonoids, terpenes, alkaloids, etc., have received considerable attention in recent years due to their diverse pharmacological properties including cytotoxic and cancer chemopreventive effects. Looking into this, the antioxidant and anticancer evaluation of Scindapsus officinalis (Roxb.) Schott fruits has been attempted to investigate its antitumor activity. The collection and authentication of the plant material mainly fruits and their various extractions was done. Identification of plant's active constituents by preliminary phytochemical screening was carried out. An in-vitro cytotoxic assay using the brine shrimp lethality assay with brine shrimp eggs (Artemia salina) at a dose of 1–10 μg/ml with the fruit extract was performed by the method described by Mayer et al. Cell viability using the Trypan blue dye exclusion test at a dose of 20, 40, 80, 120, and 160 μg/ml dissolved in DMSO (final concentration 0.1%), and cytotoxicity using the MTT assay where viable cells convert MTT into a formazan salt were performed. All pharmacological screening for acute toxicity and anti tumour studies using EAC 1 × 106 cells/mouse treated Swiss albino mice at a dose of 100 and 200 mg/kg/day orally was carried out. Biochemical and antioxidants predictions from various parameters like hematological, RBC, WBC count, PVC, total protein, Tissue Lipid Peroxidation, SOD, CATALASE, GPx, GST levels and anti tumour activity of Scindapsus officinalis were observed. The data was statistically analyzed by one-way ANOVA followed by Dunnett's and Tukey's multiple comparison test. The antitumor effect of the extract is evident from the increase in mean survival time (MST) lifespan, reduction in the solid tumor volume, and also the reversal of altered hematological parameters almost equal to normal. The methanolic extract (100–200 mg/kg/day orally) was found to be cytotoxic on human cancer cell lines. In addition, the methanolic extract had an antioxidant effect as reflected by a decrease in LPO, GST, and GPx (oxidant enzymes), and an increase in SOD and catalase.

show abstract

“…Synthetic chemotherapy can potentially cause serious side effects, the toxicity associated with the conventional cancer chemotherapy arises primarily from the lack of specificity for tumor cells. Most of the currently available anticancer drugs are designed to have selective toxicity towards rapidly dividing cells [3]. It is reported that natural compound(s) especially of plant origin, with selective cytotoxicity against specific tumor cell line, can offer either prophylaxis (chemoprevention) or a relatively safer treatment option with minimum side effect [4].…”

Section: Introductionmentioning

confidence: 99%

Crystal Structure Elucidation and Anticancer Studies of (-)-Pseudosemiglabrin: A Flavanone Isolated from the Aerial Parts of Tephrosia apollinea

et al. 2014

View full text Add to dashboard Cite

Tephrosia apollinea is a perennial shrublet widely distributed in Africa and is known to have medicinal properties. The current study describes the bio-assay (cytotoxicity) guided isolation of (-)-pseudosemiglabrin from the aerial parts of T. apollinea. The structural and stereochemical features have been described using spectral and x-ray crystallographic techniques. The cytotoxicity of isolated compound was evaluated against nine cancer cell lines. In addition, human fibroblast was used as a model cell line for normal cells. The results showed that (-)-pseudosemiglabrin exhibited dose-dependent antiproliferative effect on most of the tested cancer cell lines. Selectively, the compound showed significant inhibitory effect on the proliferation of leukemia, prostate and breast cancer cell lines. Further studies revealed that, the compound exhibited proapoptotic phenomenon of cytotoxicity. Interestingly, the compound did not display toxicity against the normal human fibroblast. It can be concluded that (-)-pseudosemiglabrin is worthy for further investigation as a potential chemotherapeutic agent.

show abstract

Selective Cytotoxicity Evaluation in Anticancer Drug Screening of Fractionated Plant Extracts

Cited by 36 publications

References 0 publications

Evaluation of toxicity and antitumor activity of cycloviolacin O2 in mice

Evaluation of toxicity and antitumor activity of cycloviolacin O2 in mice

Antioxidant and anticancer evaluation of Scindapsus officinalis (Roxb.) Schott fruits

Crystal Structure Elucidation and Anticancer Studies of (-)-Pseudosemiglabrin: A Flavanone Isolated from the Aerial Parts of Tephrosia apollinea

Contact Info

Product

Resources

About