Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix

Cheng, Hua; Sun, Guorong; Yang, Kui; Gross, Richard W.; Han, Xianlin

doi:10.1194/jlr.d004077

Cited by 73 publications

(73 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MALDI IMS and FDA of imaging data sets reveal changes in acidic lipids in the PCK rat model of ARPKD Pursuing our goal of using lipid MALDI-IMS for the discovery of candidate markers in a rat model of ARPKD, we chose 9-AA as matrix, which preferentially facilitates ionization of sulfated lipids ( 27,35 ). Zero, 4, and 10-week-old cystic PCK kidneys were compared with SDR kidneys as healthy controls ( Fig.…”

Section: Resultsmentioning

confidence: 99%

MALDI imaging MS reveals candidate lipid markers of polycystic kidney disease

Ruh

Salonikios

Fuchser

et al. 2013

Journal of Lipid Research

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

MALDI imaging MS reveals candidate lipid markers of polycystic kidney disease

Ruh

Salonikios

Fuchser

et al. 2013

Journal of Lipid Research

View full text Add to dashboard Cite

“…A mixture of 5 l of acyl CoA extract and 5 l of 9-aminoacridine solution [10 mg of 9-AA/ml in acetone/methanol (98:2, v/v)] was spotted on a MALDI plate and analyzed using a MALDI-TOF mass spectrometer ( 34 ). Quantitative analyses of acyl CoA species by MALDI-TOF-MS with 9-aminoacridine as matrix were similarly assessed as previously described ( 35,36 ) tions we use a timestep of ⌬ t ‫ف‬ 0.05-0.15. Whenever practical, a larger value of ⌬ t is used to reduce the simulation computation time.…”

Section: Acyl Coa Extraction and Analysismentioning

confidence: 99%

Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics

Kiebish

Bell

Yang

et al. 2010

Journal of Lipid Research

Self Cite

View full text Add to dashboard Cite

Lipids are critical for the regulation and control of cellular function, membrane dynamics, and signal transduction. The systematic identifi cation and analysis of all cellular lipids in a biological sample is embodied by the rapidly developing fi eld of lipidomics, which has been enabled by recent advances in mass spectrometry ( 1-3 ). A multidimensional MS-based shotgun lipidomics (MDMS-SL) approach for analysis of cellular lipidomes has been demonstrated as one of the most systematic and quantitative high-throughput platforms for global lipidomics capable of analyzing thousands of lipid molecular species directly from the lipid extracts of biological tissues ( 4, 5 ). Analysis of lipidomic data requires extensive use of bioinformatics and computational algorithms for the accurate identifi cation and quantifi cation of lipid molecular species and classes ( 5-9 ). However, most advances in bioinformatics in lipidomics have largely focused on lipid identifi cation rather than interpretation of alterations in biological function resulting in adaptive or pathologic changes in lipid metabolism ( 10 ). Thus, development of bioinformatic and systems biology approaches for the interpretation of lipidomic networks would signifi cantly advance the understanding of the roles of lipids in biological systems ( 3 ).Cardiolipin is a complex polyglycerophospholipid found almost exclusively in mitochondrial membranes of eukaryotic organisms ( 11 ). Cardiolipin is intricately involved in the effi cient production of ATP through utilizing the proton gradient as well as maintaining mitochondrial functionality ( 12-14 ). The diversity of cardiolipin molecular species varies markedly between tissues as well as among Abstract Cardiolipin is a class of mitochondrial specifi c phospholipid, which is intricately involved in mitochondrial functionality. Differences in cardiolipin species exist in a variety of tissues and diseases. It has been demonstrated that the cardiolipin profi le is a key modulator of the functions of many mitochondrial proteins. However, the chemical mechanism(s) leading to normal and/or pathological distribution of cardiolipin species remain elusive. Herein, we describe a novel approach for investigating the molecular mechanism of cardiolipin remodeling through a dynamic simulation. This approach applied data from shotgun lipidomic analyses of the heart, liver, brain, and lung mitochondrial lipidomes to model cardiolipin remodeling, including relative content, regiospecifi city, and isomeric composition of cardiolipin species. Generated cardiolipin profi les were nearly identical to those determined by shotgun lipidomics. Importantly, the simulated isomeric compositions of cardiolipin species were further substantiated through product ion analysis. Finally, unique enzymatic activities involved in cardiolipin remodeling were assessed from the parameters used in the dynamic simulation of cardiolipin profi les. Collectively, we described, verifi ed, and demonstrated a novel approach by integrating both lipidomic ana...

show abstract

“…In the last 15 years several methods have been described using TOF-secondary ion MS (19), MALDI-TOF MS (20,21), HPLC-MS (22)(23)(24)(25)(26), and direct infusion MS (15,27) for measuring ST species in different biological matrices. Quantitative and qualitative analyses of STs by LC-MS/MS have emerged as a powerful tool in research (28) and also in clinical diagnostics (29,30).…”

mentioning

confidence: 99%

High-throughput analysis of sulfatides in cerebrospinal fluid using automated extraction and UPLC-MS/MS

Blomqvist

Borén

Zetterberg

et al. 2017

Journal of Lipid Research

View full text Add to dashboard Cite

This article is available online at http://www.jlr.org ceramide (sphingoid base and a fatty acid) and a sulfated galactose. STs are, together with galactosylceramides, the most typical myelin lipids, where the long-chain fatty acid moieties C24:0 and C24:1 are expressed in particular (1, 2). In addition to myelin, STs have been shown to be expressed in subpopulations of neurons and astrocytes in the gray matter of the brain (3, 4). STs and galactosylceramides are required for the stability and maintenance of the myelin sheet (5), whereas the STs found in other brain regions suggest more diverse functions of these glycosphingolipids (6). Furthermore, STs have been suggested to participate in other cellular processes, such as protein trafficking, cell adhesion, and aggregation and immune responses, among others (7). STs are also present in many other organs, including kidney, liver, heart, intestine, muscle, and pancreas (8), although in minor amounts compared with brain, except in kidney.Findings indicate that the release of STs from myelin is associated with the development of CNS diseases and that STs in cerebrospinal fluid (CSF) might function as a marker of demyelination, myelin damage, and myelin turnover (9-13). Changes in CSF and brain ST levels have also been observed in other neurological disorders, such as early Alzheimer's disease (AD) (14-16). Furthermore, the absence of hydroxylated forms of ST in CSF could be used as a diagnostic tool for fatty acid 2-hydroxylase deficiency (17). These examples accentuate the need for a robust and sensitive method for quantification of STs in CSF.In previous studies, CSF ST measurements have usually been performed by TLC with monoclonal antibody Abstract Sulfatides (STs) are a group of glycosphingolipids that are highly expressed in brain. Due to their importance for normal brain function and their potential involvement in neurological diseases, development of accurate and sensitive methods for their determination is needed. Here we describe a high-throughput oriented and quantitative method for the determination of STs in cerebrospinal fluid (CSF). The STs were extracted using a fully automated liquid/liquid extraction method and quantified using ultra-performance liquid chromatography coupled to tandem mass spectrometry. With the high sensitivity of the developed method, quantification of 20 ST species from only 100 l of CSF was performed. Validation of the method showed that the STs were extracted with high recovery (90%) and could be determined with low inter-and intra-day variation. Our method was applied to a patient cohort of subjects with an Alzheimer's disease biomarker profile. Although the total ST levels were unaltered compared with an age-matched control group, we show that the ratio of hydroxylated/nonhydroxylated STs was increased in the patient cohort. In conclusion, we believe that the fast, sensitive, and accurate method described in this study is a powerful new tool for the determination of STs in clinical as well as preclinical settings.-Blomqvi...

show abstract

Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix

Cited by 73 publications

References 42 publications

MALDI imaging MS reveals candidate lipid markers of polycystic kidney disease

MALDI imaging MS reveals candidate lipid markers of polycystic kidney disease

Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics

High-throughput analysis of sulfatides in cerebrospinal fluid using automated extraction and UPLC-MS/MS

Contact Info

Product

Resources

About