2017
DOI: 10.1021/acs.jafc.7b00863
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Selective Detection of Shiga-like Toxin 1 from Complex Samples Using Pigeon Ovalbumin Functionalized Gold Nanoparticles as Affinity Probes

Abstract: Escherichia coli O157:H7 is a foodborne pathogen. This bacterial strain can generate Shiga-like toxins (SLTs), which can cause serious sickness and even death. Thus, it is important to develop effective and sensitive methods that can be used to rapidly identify the presence of SLTs from complex samples. Pigeon egg white (PEW) contains abundant glycoproteins, including pigeon ovalbumin (POA) (∼60%). POA possesses Gal-α(1→4)-Gal-β(1→4)-GlcNAc termini, which can recognize the B subunits in SLT type 1 (SLT-1B). Th… Show more

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Cited by 9 publications
(6 citation statements)
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“…The maximum absorption band appeared at the wavelength of $520 nm (Figure 1(A)), and the generated POA-Au NPs had red color (inset photograph in Figure 1(A)). The generated POA-Au NPs have similar UV-Vis absorption spectrum and color to that in the previous reported [40]. Figure 1(B) shows the transmission electron microscope (TEM) image of the generated POA-Au NPs.…”
Section: Resultssupporting
confidence: 70%
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“…The maximum absorption band appeared at the wavelength of $520 nm (Figure 1(A)), and the generated POA-Au NPs had red color (inset photograph in Figure 1(A)). The generated POA-Au NPs have similar UV-Vis absorption spectrum and color to that in the previous reported [40]. Figure 1(B) shows the transmission electron microscope (TEM) image of the generated POA-Au NPs.…”
Section: Resultssupporting
confidence: 70%
“…Characterization of POA-Au NPs POA-Au NPs were prepared according to the method reported previously [40]. The generated POA-Au NPs were first characterized by ultraviolet-visible (UV-Vis) absorption spectroscopy.…”
Section: Resultsmentioning
confidence: 99%
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