Selective detections of Hg2+ and F− by using tailor-made fluorogenic probes

Abstract

By ingeniously using a (imino)coumarin-precursor, three reactive fluorogenic probes of MP, FP, and FMP have been fabricated in a single facile synthetic route. MP and FP are able to respectively act as selective "turn-on" fluorescent probes for detecting Hg 2+ and Fin buffer solution via specific analyte-induced reactions. Linear ranges for the detection of Hg 2+ and Fare 0-10 µM and 0-100 µM with the limits of detection (LODs) of 4.0 × 10 -8 M and 1.14 × 10 -6 M (3δ/slope), respectively. FMP is able to work as a molecular "AND" logic gate-based fluorogenic probe for monitoring the coexistence of Hg 2+ and Fvia a multistep reaction cascade.The analytes-induced sensing mechanisms have been determined by using high-performance liquid chromatography analysis (HPLC). In addition, three probes show negligible toxicity under the experimental conditions, and are successfully used for monitoring Hg 2+ and Fin living cells with good cell permeability. The success of the work demonstrates that ingenious utility of specific analyte-induced reactions and conventional concepts on the appropriate molecular scaffold can definitely deliver tailor-made probes for various intended sensing purposes.

A fluorescent probe for the dual detection of mercury ions and thiols based on a simple coumarin derivative

“…For example, certain bifunctional fluorescent probes were capable of sensing Hg(ii) along with another analyte such as Ag(i), 26,27 Cu(ii), [28][29][30] Fe(iii), 31 Al(iii), 32 pH change, 33 saccharides 34 or F -. 35,36 A novel chlorinated coumarin-malononitrile fluorescent probe with three potential reaction sites was developed, and it demonstrated a highly selective and rapid response to hydrogen sulphide and GSH. 37 It is known that thiophilic Hg(ii) has a strong binding ability with the sulphur atom in biothiols and thus has the potential to disturb the biothiol level in the physiological system.…”

“…Recently, efficient bifunctional fluorescent probes garnered substantial interest because they can be used for the simultaneous detection of two different analytes. For example, certain bifunctional fluorescent probes were capable of sensing Hg( ii ) along with another analyte such as Ag( i ), Cu( ii ), Fe( iii ), Al( iii ), pH change, saccharides or F – . A novel chlorinated coumarin‐malononitrile fluorescent probe with three potential reaction sites was developed, and it demonstrated a highly selective and rapid response to hydrogen sulphide and GSH .…”

A Pyrazole‐Based Highly Selective Colorimetric Chemosensor for Hg2+ Ion in Semi‐Aqueous Medium

“…Recently, several scientific reports have been made for the detection of Hg 2 + ion and it is interfering with other metal ions or anions. [33][34][35][36][37][38][39] Most of the reported probes are fluorogenic quencher or enhancer within the presence of Hg 2 + . [40][41][42] Moreover, the nanomaterial based sensors designed for recognition of Hg 2 + on the nanomaterial surface and very few kinds of literature are available for colorimetric detection of Hg 2 + .…”

“…Recently, several scientific reports have been made for the detection of Hg 2+ ion and it is interfering with other metal ions or anions . Most of the reported probes are fluorogenic quencher or enhancer within the presence of Hg 2+ .…”

Long-Wavelength Fluorescent Chemosensors for Hg2+ based on Pyrene

“…7−9 At present, a lot of chemosensors toward Hg 2+ have been designed, synthesized, and characterized. 10−13 In these chemosensors, there are three aspects to be improved: (1) many chemosensors recognize Hg 2+ through the “on–off” fluorescent type. However, the type is easy to give false positive results.…”