Selective determination of cysteine by resonance light scattering technique based on self-assembly of gold nanoparticles

Rapid and selective detection of cysteine based on its induced aggregates of cetyltrimethylammonium bromide capped gold nanoparticles

“…In this work, we employed the CTAB-capped Au-NPs to detect cysteine in the medium of high salt concentration, which is impossible for citrate-coated Au-NPs, since the citratecoated ones display strong aggregation tendency in high salt medium. Among amino acids found in proteins, cysteine is the only one that with -SH group, and plays very important roles in physiology both for animals [6,[13][14][15] and plants [16,17]. Therefore, detection of cysteine is very significant.…”

“…By now, many methods have been applied to detect cysteine, such as high-performance liquid chromatography (HPLC) [18], electrochemistry [19], chemiluminescence [20], flow-injection [21], colorimetry [22], and spectrophotometry [23], all of which are based on the redox reaction or derivation with chromophores or fluorophores. Of course, there are also some methods based upon the formation of Au-S covalent bond between cysteine and gold nanorods [24] or Au-NPs [6,7,25,26], with the red shift of plasmon resonance absorption band of Au-NPs [7], disappearance of S-H stretching in both the IR and Raman spectra, and variations of chemical shift of the 1 H NMR in close proximity to the metal center [26].…”

“…For these features, they have been applied to comprehensive studies including coding of single-nucleotide polymorphisms [1], regulating intracellular gene [2], and detecting the hybridization of DNA and SNPs [3,4], duplex and triplex DNA [5], amino acids [6][7][8], and glucose [9]. Generally, Au-NPs are prepared by means of reducing of HAuCl 4 by citrate sodium, and coated with citrate group.…”

A kinetic method for the determination of organosulfur compounds by inhibition: determination of cysteine, 2,3-dimercaptopropanol and thioglycolic acid

“…Due to biological and pharmaceutical significance of cysteine, a variety of approaches have been developed to determine its concentration in different kinds of samples [1][2][3][8][9][10][11][12][13][14][15]. A few methods have been proposed for the determination of TGA in different samples [16][17][18].…”

Spectrophotometric Determination of Cysteine with Gold Nanoparticles Stabilized with Single-stranded Oligonucleotides

“…Until now, a variety of methods to determine cysteine level have been developed, including high-performance liquid chromatography (HPLC), 6,7 electrochemistry, [8][9][10] fluorometry, [11][12][13] and resonance light scattering. 14,15 However, most of them require complicated instrumentation, have relatively high analytical costs, and involve time-consuming pretreatment steps prior to the analysis; these difficulties limit the scope of their practical applications. Therefore, new analytical methods from alternative techniques are always useful, especially if the methods are simple, cost-effective and comparatively fast.…”