Selective Determination of Nicotinamide Adenine Dinucleotide in the Presence of Ascorbic Acid and Uric Acid at a Long-Length Carbon Nanotube Electrode

Abstract

Selective electrochemical determination of nicotinamide adenine dinucleotide (NADH) in the presence of ascorbic acid (AA) and uric acid (UA) at a long-length multi-walled carbon nanotube (CNT) electrode is presented. We demonstrate the effectiveness of CNT length toward the electrochemical response due to oxidation of NADH. The long-length CNT is 200 µm (average), whereas normal-length CNT as a comparison is 1 µm (average). In differential pulse voltammetry, the current peaks due to NADH, AA, and UA at long-length CNT electrodes were more distinct than those at normal-length electrode. Electrochemical impedance spectroscopy measurements suggested that the long-length CNT electrode formed a better electron transfer network than the normal-length electrode. The performance of selective determination of NADH are concentration range of 0.030-2.0 mM in the presence of 1.2 mM AA, and 0.059-2.0 mM in the presence of 0.12 mM UA.

Determination of the Electrochemical Area of Screen-Printed Electrochemical Sensing Platforms

“…The tested solutions were: 1 mM N , N , N ′, N ′-tetramethyl- p -phenylenediamine (TMPD) in 0.1 M KCl, 1 mM Ru(NH 3 ) 6 Cl 3 3+/2+ (RuHex) in 0.1 M KCl, 1 mM Dopamine in pH 7 phosphate buffer solution/0.1 M KCl (PBS), 1 mM β -Nicotinamide adenine dinucleotide (NADH) in pH 7 PBS/0.1 M KCl, 1 mM Capsaicin in 0.1 M HPO 4 , and 1 mM Ascorbic acid in pH 7 PBS/0.1 M KCl. The following diffusion coefficients were used in this work (cm 2 s −1 ): 6.74 × 10 −6 for dopamine [ 21 ], 7.40 × 10 −6 for NADH [ 22 ], 9.1 × 10 −6 for RuHex [ 23 ], 6.32 × 10 −6 for TMPD [ 24 ], 7.03 × 10 −6 for capsaicin, and 1.42 × 10 −6 for ascorbic acid [ 25 ].…”

Electrochemical Characterization of Nicotinamide Riboside

“…[2,3] In particular, NAD + determines the activity of enzymes, such as sirtuin enzymes, which are crucial to cellular metabolism, cell survival, and DNA repair. [2,3,6] This has become apparent as the decline of the cofactor has been attributed to various disease states, such as cardiovascular disease, diabetes, and kidney failure. [2,3,6] This has become apparent as the decline of the cofactor has been attributed to various disease states, such as cardiovascular disease, diabetes, and kidney failure.…”

“…[1] NAD + /NADH cofactor is critical for the success of the catalytic behavior of dehydrogenase enzymes, while also functioning as a biomarker within various systems and a regulatory agent for post-translational protein modifications. [2,3] In particular, NAD + determines the activity of enzymes, such as sirtuin enzymes, which are crucial to cellular metabolism, cell survival, and DNA repair. [4,5] The pyridine nucleotide is essential for the production and consumption of energy, and decreases in abundance as we age.…”

“…[4,5] The pyridine nucleotide is essential for the production and consumption of energy, and decreases in abundance as we age. [2,3,6] This has become apparent as the decline of the cofactor has been attributed to various disease states, such as cardiovascular disease, diabetes, and kidney failure. [7] To highlight physiological relevance further, sirtuin enzymes use the cofactor to deacetylate the lysine groups of histones and proteins of various subcellular components.…”

Selective detection of rutin in the presence of ascorbic acid with a carbon nanotube electrode

“…26,27) Furthermore, we demonstrated that a long-length CNT electrode showed better electrochemical performance than its normal-length counterparts. [28][29][30] Therefore, we would like to extend the possibility of the CMC/long-length CNT electrode. We demonstrate the selective determination with a CNT electrode of rutin in the presence of ascorbic acid (AA), which now often coexists with rutin in soba tea in a PET bottled beverage.…”