Selective effects of hydroxyapatite nanoparticles on osteosarcoma cells and osteoblasts

Qing, Fangzhu; Wang, Zhe; Hong, Youliang; Liu, Ming; Guo, Bo; Luo, Hongrong; Zhang, Xingdong

doi:10.1007/s10856-012-4703-6

Cited by 63 publications

(56 citation statements)

References 12 publications

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“…Interestingly, cytotoxic effects were observed with increasing HAPna concentration to 1.0 mg/mL HAPna. HAP cytotoxicity has been reported previously (Qing et al, 2012;Sun et al, 1997), in particular Sun et al, (1997) demonstrated that osteoblast cellular proliferation was significantly reduced when cultured in media containing HAP powder at a concentration of 1.0 mg/mL, as was used in this study, thus highlighting the importance of testing nanoparticle concentrations over a narrow range of the desired concentration to assess potential toxicity (Sun et al, 1997).…”

Section: Cytocompatibility Assessment Of Hydroxyapatite Hydrogel Scafsupporting

confidence: 66%

Hydroxyapatite nanoparticle injectable hydrogel scaffold to support osteogenic differentiation of human mesenchymal stem cells

Thorpe

Creasey²,

Sammon³

et al. 2016

eCM

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Bone loss associated with degenerative disease and trauma is a clinical problem increasing with the aging population. Thus, effective bone augmentation strategies are required; however, many have the disadvantages that they require invasive surgery and often the addition of expensive growth factors to induce osteoblast differentiation. Here, we investigated a Laponite  crosslinked, pNIPAMDMAc copolymer (L-pNIPAM-co-DMAc) hydrogel with hydroxyapatite nanoparticles (HAPna), which can be maintained as a liquid ex vivo, injected via narrowgauge needle into affected bone, followed by in situ gelation to deliver and induce osteogenic differentiation of human mesenchymal stem cells (hMSC). L-pNIPAMco-DMAc hydrogels were synthesised and HAPna added post polymerisation. Commercial hMSCs from one donor (Lonza) were incorporated in liquid hydrogel, the mixture solidified and cultured for up to 6 weeks. Viability of hMSCs was maintained within hydrogel constructs containing 0.5 mg/mL HAPna. SEM analysis demonstrated matrix deposition in cellular hydrogels which were absent in acellular controls. A significant increase in storage modulus (G') was observed in cellular hydrogels with 0.5 mg/mL HAPna. Semi-quantitative immunohistochemistry and histological analysis demonstrated that bone differentiation markers and collagen deposition was induced within 48 h, with increased calcium deposition with time. The thermally triggered hydrogel system, described here, was sufficient without the need of additional growth factors or osteogenic media to induce osteogenic differentiation of commercial hMSCs. Preliminary data presented here will be expanded on multiple patient samples to ensure differentiation is seen in these samples. This system could potentially reduce treatment costs and simplify the treatment strategy for orthopaedic repair and regeneration.

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Section: Cytocompatibility Assessment Of Hydroxyapatite Hydrogel Scafsupporting

confidence: 66%

Hydroxyapatite nanoparticle injectable hydrogel scaffold to support osteogenic differentiation of human mesenchymal stem cells

Thorpe

Creasey²,

Sammon³

et al. 2016

eCM

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“…The potential toxicity or biocompatibility of HA particles depends on their concentration. In a study by Qing et al, 42 it was found that concentrations of HA nanoparticles ranging from 100 to 500 µg/mL reduce the growth and the viability of MG-63 cells. At a concentration of 100 µg/mL, HA nanoparticles are found in cell lysosomes, and concentrations greater than 250 µg/mL causes apoptotic changes in the nuclei of MG-63 cells.…”

mentioning

confidence: 99%

“…HA nanoparticles suspended in a cell culture medium slightly support the proliferation of primary human osteoblasts but inhibit the growth of MG-63 cells and induce apoptosis in this cell type. 42 Previously, composites 0, N2-N25, and M2-M5 were tested for mechanical properties, 22 aimed at finding a suitable ratio of HA nano/microparticles to optimize the mechanical properties to be comparable with that of human cortical bone. The modulus of elasticity in bending of the composites is similar to that in cortical bone, which is 14-20 GPa.…”

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confidence: 99%

Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

Filová¹,

Suchý²,

Sucharda³

et al. 2014

IJN

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Hydroxyapatite (HA) is considered to be a bioactive material that favorably influences the adhesion, growth, and osteogenic differentiation of osteoblasts. To optimize the cell response on the hydroxyapatite composite, it is desirable to assess the optimum concentration and also the optimum particle size. The aim of our study was to prepare composite materials made of polydimethylsiloxane, polyamide, and nano-sized (N) or micro-sized (M) HA, with an HA content of 0%, 2%, 5%, 10%, 15%, 20%, 25% (v/v) (referred to as N0–N25 or M0–M25), and to evaluate them in vitro in cultures with human osteoblast-like MG-63 cells. For clinical applications, fast osseointegration of the implant into the bone is essential. We observed the greatest initial cell adhesion on composites M10 and N5. Nano-sized HA supported cell growth, especially during the first 3 days of culture. On composites with micro-size HA (2%–15%), MG-63 cells reached the highest densities on day 7. Samples M20 and M25, however, were toxic for MG-63 cells, although these composites supported the production of osteocalcin in these cells. On N2, a higher concentration of osteopontin was found in MG-63 cells. For biomedical applications, the concentration range of 5%–15% (v/v) nano-size or micro-size HA seems to be optimum.

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“…Ultrasonic enhanced hydroxyapatite into cells, and increased the intracellular HA concentration, then the tumor can be inhabited by low HA concentration. HA can be dissolved at the aid environment, the phosphate radical and calcium ion release of HA, resulting the increase of the intracellular calcium concentration, and induced the tumor cell apoptosis [20,21] . Beyond that, HA can affect the function of organelles in cytoplasm, cell cycle block, the inhibition of telomerase activity , mitochondrial function injury, DNA damage, and protein synthesis [22,23] .…”

Section: Discussionmentioning

confidence: 99%

Apoptosis for HepG2 Cells Induced by Low-frequency and Low-power Ultrasound with Hydroxyapatite

Chen¹,

Xu²,

Wang³

et al. 2017

Proceedings of the 2017 International Conference on Material Science, Energy and Environmental Engineering (MSEEE 2017)

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Abstract. The exploration of the apoptosis for HepG2 cells induced by low-frequency and low-power ultrasound combined with hydroxyapatite, provides data base for future clinical treatment. In this research, we selected the HepG2 cells model. The experiences were divided into four groups: control group, ultrasound group, hydroxyapatite group, and ultrasound + hydroxyapatite group. Ultrasound group and control group replaced the normal culture medium, hydroxyapatite group and ultrasound + hydroxyapatite group replaced culture medium containing hydroxyapatite. The control group and hydroxyapatite group were not treated, while ultrasound group and ultrasound + hydroxyapatite group were treated by ultrasound with different parameters (f= 20 kHz; P=0.1, 0.2, 0.3w/cm 2 ; t=30, 60, 90, 120, 150, 180s; T= 0, 12, 24, 48, 72h). MTT test and flow cytometry detected the inhibitory rate of the cells, cell apoptosis and cell cycle. MTT experiment results show that ultrasound with different parameters stimulates the cell model, which leads to the difference of the inhibition rate of cell growth. Among them, the inhibition rate of cell growth reached the top value (71.726±0.677)% with the treatment of f=20 kHz, P =0.2w/cm 2 ,t=90s,T=48h. There was a statistical significance compared with the control group, ultrasound group, and hydroxyapatite group (P<0.05).Under such condition, the apoptosis rate of HepG2 cells was a statistically significant change compared with the control group, ultrasound group, and hydroxyapatite group (P<0.05). During the cell cycle assay, the ultrasonic + hydroxyapatite group also showed obvious G2/M phase block. Low frequency and low-power ultrasound with Hydroxyapatite induced the apoptosis in human HepG2 cells.

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Selective effects of hydroxyapatite nanoparticles on osteosarcoma cells and osteoblasts

Cited by 63 publications

References 12 publications

Hydroxyapatite nanoparticle injectable hydrogel scaffold to support osteogenic differentiation of human mesenchymal stem cells

Hydroxyapatite nanoparticle injectable hydrogel scaffold to support osteogenic differentiation of human mesenchymal stem cells

Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

Apoptosis for HepG2 Cells Induced by Low-frequency and Low-power Ultrasound with Hydroxyapatite

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