Selective enhancement of Raman or fluorescence spectra of biomolecules using specifically annealed thick gold films

Strekal, N. D.; Маскевич, А. А.; Маскевич, С. А.; Jardillier, Jean‐Claude; Nabiev, Igor

doi:10.1002/1097-0282(2000)57:6<325::aid-bip10>3.0.co;2-7

Cited by 44 publications

(43 citation statements)

References 6 publications

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“…2c (spectra 3 and 4). As reported previously, PGF-350 enhance Raman scattering of adsorbed mitox, while PGF-290 enhance mitox fluorescence, so we have associated this selectivity of secondary emission enhancement with different overlap of molecular electronic transitions and localized plasmon band under resonance excitation [12]. Spectrum 5 in Fig.…”

Section: Resultssupporting

confidence: 58%

“…Plasmonic film in contrast to plasmonic crystal [9][10][11] means disordered nanotextured metal surface in which SPP and/or localized plasmons (LP) could be excited under resonance conditions. In spite of non-periodic structure, plasmonic films possess some unique properties such as electromagnetic field enhancement and the selectivity of the enhancement effect at the certain frequencies within plasmon band as it was shown previously [12].…”

Section: Introductionmentioning

confidence: 73%

“…Afterward, the PGFs were annealed in the air at the muffle oven at the required temperature (350 or 290°C). PGFs preparation was described previously [12].…”

Section: Methodsmentioning

confidence: 99%

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Features of the Secondary Emission Enhancement Near Plasmonic Gold Film

et al. 2008

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Plasmonic gold films (PGF) prepared by vacuum deposition of gold onto quartz slides possess unique property to enhance electromagnetic signal in the near field. Spectral tuning of PGF's plasmon band to resonance with the electronic spectra of adsorbed molecules provides selective enhancement of fluorescence or surface-enhanced Raman scattering in the far field. Plasmon-enhanced fluorescence (PEF) of mitoxantrone (mitox) as a function of the distance between gold surface and adsorbed molecules for different polarization and incidence angle of exciting light is analyzed in this work. Spectrophotometric data reveal that probability of localized plasmon excitation in gold grains increases with growth of incidence angle for s-polarized and decrease for p-polarized excitation. This fact correlates well with oblate shape of gold particles detected by Atomic force microscope. However, the fluorescence intensity of dyes deposited at fixed distance from gold surface increase with angle of incidence of p-polarized light more noticeably than for s-polarized one. Nevertheless, the behavior of mitox PEF signal upon p-polarized laser excitation and different angle of incidence are similar in appearance to such phenomenon as selective photoelectric effect. According to this observation, the nearfield interactions between plasmons and molecule as possible mechanism of PEF is discussed.

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Section: Resultssupporting

confidence: 58%

Section: Introductionmentioning

confidence: 73%

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Features of the Secondary Emission Enhancement Near Plasmonic Gold Film

et al. 2008

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“…It has been found that coatings with predictable morphological and optical characteristics can be obtained by controlling the parameters of the processes for producing these gold films (deposition time for the nanoparticles, their concentration, the number of polyelectrolyte sublayers) [9]. This makes it possible to select by technical means the most efficient substrate for enhancing the luminescence intensity of a given adsorbate, while maintaining maximal overlap of the absorption spectrum of the fluorophore with the plasmon band of the metal film at the excitation frequency [13].…”

Section: Introductionmentioning

confidence: 99%

Production of colloidal nanostructures for optical and spectral-analytic applications

2011

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The production of composite nanostructures by self-assembly of monodisperse nanoparticles (SiO 2 , Au, CdSe/ZnS) and polyelectrolytes is described. The efficiency of using specially developed metal-dielectric films as substrates for obtaining surface-enhanced Raman scattering and enhanced photoluminescence of adsorbates is demonstrated. Photostable nanofilaments and ring structures are made out of fluorescent semiconducting CdSe/ZnS nanocrystals. Introduction.In recent decades methods based on the principles of self-organization of matter and self-assembly have been an important way of obtaining new nanostructures. They have a number of advantages over other methods of producing nanostructures (nanolithography, physical grinding, etc.), since a set of fairly simple nanochemical reagents can be used to fabricate composite structures with specified properties which are organized in ways required for applications in optics [1], catalysis [2], nanoelectronics [3], nanophotonics [4], and other areas of science and technology.Here we describe some methods that we have developed for producing composite metal-dielectric and semiconducting nanostructures for optical and spectral-analytic applications. These methods are based on the principles of self-assembly of nanometer sized objects. The resulting thin-film nanostructures include substrates containing metals which can be used in (surface-) enhanced Raman scattering spectroscopy and for increasing the luminescence intensity of adsorbates (detecting so-called "surface-enhanced luminescence"), as well as two-dimensional structures made up of luminescent semiconductor CdSe/ZnS core-shell nanocrystals. This topic is of great interest because of the wide range of unsolved problems in rapidly developing areas of nano-optics and nanotechnology, especially the need to develop highly reproducible fabrication techniques for systems with unique and controllable optical properties. Experiment. Self-organization and self-assembly methods have been used to obtain the following kinds of nanostructures:(a) ordered colloidal crystals as templates for creating metallic films with regular roughnesses; (b) colloidal gold films with controllable optical properties based on monodisperse nanoparticles and polyelectrolytes;(c) multilayer films based on polyelectrolytes as separator dielectric layers between an analyte and a metal to prevent quenching of photoluminescence; and, (d) two-dimensional structures made up of fluorescent semiconducting nanocrystals -ring structures and nanofilaments.Polydiallyl methyl ammonium chloride (polycation, M=200,000 g/mol) and sodium polystyrol sulfonate (polyanion, M = 70,000 g/mol) have been used as polyelectrolytes for layer-by-layer deposition on substrates from water solutions. Deposition was done by initially processing each metal substrate in a 1 g/liter polycation solution with 0.5

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“…In particular, in order to study the fluorescence as a function of the distance between the molecule and the metal, different intermediate dielectric layers (spacers) are used (proteins, phospholipids, organic acids, amines, and their derivatives [8,9], deposited on the metal substrate by the Langmuir-Blodgett method; polymers (polymethylmethacrylate, polylysine [8]), quartz [9], making it possible to controllably vary the distance from the luminophore to the metal.…”

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confidence: 99%

Improved fluorescent assay sensitivity using silver island films: Fluorescein isothiocyanate-labeled albumin as an example

et al. 2006

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We have studied enhancement of the fluorescence of fluorescein isothiocyanate (FITC), bound to albumin and near an annealed silver island film, as a function of the distance between the protein molecules and the metal. As the intermediate spacer layer between the albumin and the silver substrate, we used multilayer films based on polyelectrolytes. The maximum nine-fold enhancement coefficient for the fluorescence of FITC corresponds to a thickness of the intermediate layer of ≈4 nm, or three layers of the polyelectrolyte. In this case, we observe a significant decrease in the average photoluminescence decay time for the label near the silver film compared with a dielectric medium. Key words: enhanced fluorescence; island silver film; intermediate layer (spacer); bovine serum albumin labeled by fluorescein isothiocyanate.Introduction. Modification of the photoluminescence (FL) of atoms and molecules located near nanostructured metallic surfaces [1-6] is an important and promising phenomenon in the optics of nanostructures. Detailed study of these processes is important both for understanding the fundamental aspects of the interaction between light and matter and for solving applied problems in nanotechnology and spectroscopy, in particular for designing new efficient luminophores and improving the sensitivity of the fluorescent method in enzyme immunoassays.In contrast to giant Raman scattering (GRS), widely studied since the 1980s, where typically maximum signal enhancement occurs when the molecules are adsorbed directly on rough metallic substrates, enhancement of the luminescence signal occurs when the molecule is positioned at some distance from a metallic substrate, as was predicted theoretically in [7]. This effect is due to two specific processes: 1) enhancement of the luminescence of the molecules due to excitation of surface plasmons in the metal and redistribution of the density of photon states; 2) quenching of the luminescence signal as a consequence of nonradiative energy transfer from the molecule to the metal.To date, surface-enhanced optical phenomena (giant Raman scattering, enhancement of luminescence, enhancement of second harmonic generation) and their mechanisms and the factors affecting the enhancement are under intense study, and the search is underway to find the conditions for obtaining the greatest possible enhancement factor. In particular, in order to study the fluorescence as a function of the distance between the molecule and the metal, different intermediate dielectric layers (spacers) are used (proteins, phospholipids, organic acids, amines, and their derivatives [8, 9], deposited on the metal substrate by the Langmuir-Blodgett method; polymers (polymethylmethacrylate, polylysine [8]), quartz [9], making it possible to controllably vary the distance from the luminophore to the metal.It was suggested earlier that multilayer structures based on polyelectrolytes be used as the intermediate layer [4], where the process of deposition onto the metal surface is based on electrostatic self...

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Selective enhancement of Raman or fluorescence spectra of biomolecules using specifically annealed thick gold films

Cited by 44 publications

References 6 publications

Features of the Secondary Emission Enhancement Near Plasmonic Gold Film

Features of the Secondary Emission Enhancement Near Plasmonic Gold Film

Production of colloidal nanostructures for optical and spectral-analytic applications

Improved fluorescent assay sensitivity using silver island films: Fluorescein isothiocyanate-labeled albumin as an example

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