Selective enrichment of ailanthone from leaves of ailanthus altissima by tandem reverse phase/molecularly imprinted solid phase extraction

Anfossi, Laura; Giovannoli, Cristina; Nardo, Fabio Di; Cavalera, Simone; Chiarello, Matteo; Trotta, Francesco; Baggiani, Claudio

doi:10.1016/j.microc.2020.105198

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…The leaves of A. altissima are known for their high content of polyphenols, molecules with strong antioxidant properties, followed by lower amounts of other compounds, such as alkaloids, sesquiterpene and triterpenoids (ailanthone), proteins, carbohydrates, minerals, etc. [ 17 , 18 ], making leaves of such species excellent candidates for diverse applications (antimicrobials, biopesticides) [ 19 ]. However, extracts from such invasive plant species have been less investigated for their level of impact on the environment or human health, generally because natural products are mostly perceived as safe.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Screening of Ecotoxic and Cytotoxic Activities of Ailanthus altissima Leaf Extract against Target and Non-Target Plant and Animal Cells

Cocîrlea,

Simionescu,

Petrovici

et al. 2024

IJMS

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Ailanthus altissima, an invasive plant species, exhibits pharmacological properties, but also some allergic effects on humans. This study aimed to evaluate the potential toxicity of A. altissima leaves, using a complex approach towards different organisms. The ecotoxic impact of a crude extract was investigated on seeds germination and brine shrimp lethality. Cytotoxicity was studied in vitro using non-target (haemolysis, liposomal model, fibroblast), and target (cancer cells) assays. Leaf extract at 1000 µg/mL significantly inhibited wheat and tomato germination, while no significant effects were found on parsley germination. A slight stimulatory effect on wheat and tomato germination was found at 125 µg/mL. In a brine shrimp-test, the extract showed a low toxicity at 24 h post-exposure (LC50 = 951.04 ± 28.26 μg/mL), the toxic effects increasing with the exposure time and extract concentration. Leaf extract caused low hematotoxicity. The extract was biocompatible with human gingival fibroblasts. No anti-proliferative effect was found within the concentration range of 10–500 µg/mL on malignant melanoma (MeWo) and hepatocellular carcinoma (HepG2). In a liposomal model-test, the extract proved to possess low capability to alter the eukaryotic cell-mimicking membranes within the tested concentration range. Given the low to moderate toxicity on tested organisms/cells, the A. altissima autumn leaves may find useful applications.

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Section: Introductionmentioning

confidence: 99%

In Vitro Screening of Ecotoxic and Cytotoxic Activities of Ailanthus altissima Leaf Extract against Target and Non-Target Plant and Animal Cells

Cocîrlea,

Simionescu,

Petrovici

et al. 2024

IJMS

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Investigation of chemical composition of Ailanthus Altissima (Mill.) Swingle

Панасенко

Aksonova

Denysenko

et al. 2020

Current Issues in Pharmacy and Medicine: Science and Practice

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Phenolic Composition and Bioactivities of Invasive Ailanthus altissima (Mill.) Swingle Leaf Extracts Obtained by Two-Step Sequential Extraction

Cocîrlea,

Soare,

Petrovici

et al. 2024

Antioxidants

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Ailanthus altissima, a highly invasive species, contains valuable compounds in different plant parts, indicating great practical potential. This paper proposes the use of non-polar (n-hexane) and polar (ethanol) solvents for the extraction of antioxidant compounds from A. altissima (family Simaroubaceae) leaves in a sequential two-step process. Fresh and dried leaves were examined for their microstructure by scanning electron microscopy, and for color changes in the CIELAB color space co-ordinates. An investigation of the harvesting season, processing (freezing and drying), and solvent indicates ethanol can be used for the highly efficient extraction of phenolics, flavonoids, tannins, and carotenoids. Statistically significant differences were found between the autumn and summer samples for phenolic content, and between dried and frozen samples for tannin content. The HPLC phenolic profile indicates more phenolics (nine polyphenols) in dried leaves harvested in both seasons compared to those in frozen ones (five to six polyphenols). Frozen leaves showed a higher antioxidant activity in a ferric-reducing antioxidant power assay than that of the dried samples, which exhibited a higher antioxidant activity using the 1, 1-diphenyl-2-picryl-hydrazyl assay, but it was not statistically significant. The phenolic, flavonoid, and carotenoid contents significantly influenced the antioxidant activities. Among the ethanolic extracts, those from dried leaves showed better antibacterial activity, in particular, on Staphylococcus aureus and Enterococcus faecalis. The high bioactive content and activity of A. altissima leaves make them suitable natural raw materials for various applications.

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Selective enrichment of ailanthone from leaves of ailanthus altissima by tandem reverse phase/molecularly imprinted solid phase extraction

Cited by 3 publications

References 24 publications

In Vitro Screening of Ecotoxic and Cytotoxic Activities of Ailanthus altissima Leaf Extract against Target and Non-Target Plant and Animal Cells

In Vitro Screening of Ecotoxic and Cytotoxic Activities of Ailanthus altissima Leaf Extract against Target and Non-Target Plant and Animal Cells

Investigation of chemical composition of Ailanthus Altissima (Mill.) Swingle

Phenolic Composition and Bioactivities of Invasive Ailanthus altissima (Mill.) Swingle Leaf Extracts Obtained by Two-Step Sequential Extraction

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