Selective femtosecond laser ablation via two-photon fluorescence imaging through a multimode fiber

Kakkava, Eirini; Romito, Marilisa; Conkey, Donald B.; Loterie, Damien; Stanković, Konstantina M.; Moser, Christophe

doi:10.1364/boe.10.000423

Cited by 41 publications

(18 citation statements)

References 43 publications

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“…Our approach offers significant imaging flexibility because the ablation sites can be strategically placed along the cochlear length to interrogate cochlear regions encoding specific acoustic frequencies. Our methodology naturally suits a fiber-based endoscopic implementation that integrates capabilities of both ultrafast laser ablation [63,64] and TPEF imaging [65,66].…”

Section: Resultsmentioning

confidence: 99%

Imaging hair cells through laser-ablated cochlear bone

Romito¹,

Pu²,

Stanković³

2019

Biomed. Opt. Express

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We report an innovative technique for the visualization of cells through an overlying scattering medium by combining femtosecond laser bone ablation and two-photon excitation fluorescence (TPEF) microscopy. We demonstrate the technique by imaging hair cells in an intact mouse cochlea ex vivo. Intracochlear imaging is important for the assessment of hearing disorders. However, the small size of the cochlea and its encasement in the densest bone in the body present challenging obstacles, preventing the visualization of the intracochlear microanatomy using standard clinical imaging modalities. The controlled laser ablation reduces the optical scattering of the cochlear bone while the TPEF allows visualization of individual cells behind the bone. We implemented optical coherence tomography (OCT) simultaneously with the laser ablation to enhance the precision of the ablation and prevent inadvertent damage to the cells behind the bone.

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Section: Resultsmentioning

confidence: 99%

Imaging hair cells through laser-ablated cochlear bone

Romito¹,

Pu²,

Stanković³

2019

Biomed. Opt. Express

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“…In recent years, spatial control of light propagation in MMFs has been widely achieved, in the linear regime, via iterative methods, phase conjugation and the transmission matrix method for applications such as imaging and material processing 16,17 . Recently, machine learning tools have been proposed to simplify the calibration and improve the robustness of the system in the absence of optical nonlinearities 18 .…”

Section: Introductionmentioning

confidence: 99%

Controlling spatiotemporal nonlinearities in multimode fibers with deep neural networks

et al. 2020

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Spatiotemporal nonlinear interactions in multimode fibers are of interest for beam shaping and frequency conversion by exploiting the nonlinear propagation of different pump regimes from quasi-continuous wave to ultrashort pulses centered around visible to infrared pump wavelengths. The nonlinear effects in multi-mode fibers depend strongly on the excitation condition, however relatively little work has been reported on this subject. Here, we present the first machine learning approach to learn and control the nonlinear frequency conversion inside multimode fibers by tailoring the excitation condition via deep neural networks. Trained with experimental data, deep neural networks are adapted to learn the relation between the spatial beam profile of the pump pulse and the spectrum generation. For different user-defined target spectra, network-suggested beam shapes are applied and control over the cascaded Raman scattering and supercontinuum generation processes are achieved. Our results present a novel method to tune the spectra of a broadband source.

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“…Preliminary studies on colon tissue resection based on ultrafast laser pulses delivered via a galvanometer have been reported recently . Researchers have used multicore and graded index multimode fibres for the delivery of ultrashort pulse (USP) to demonstrate the laser ablation. Prior work has established pig tissue as an efficient medical model for several studies related to human diseases and is consequently applied in porcine vocal fold tissue resection using 1.5 ps laser pulses delivered through kagome lattice hollow core photonic crystal fibre for microsurgery .…”

Section: Introductionmentioning

confidence: 99%

Preclinical evaluation of porcine colon resection using hollow core negative curvature fibre delivered ultrafast laser pulses

Mohanan

Beck

West

et al. 2019

Journal of Biophotonics

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Ultrashort pulse lasers offer great promise for tissue resection with exceptional precision and minimal thermal damage. Surgery in the bowel requires high precision and minimal necrotic tissue to avoid severe complications such as perforation. The deployment of ultrashort lasers in minimally invasive or endoscopic procedures has been hindered by the lack of suitable optical fibres for high peak powers. However, recent developments of hollow core microstructured fibres provide potential for delivery of such pulses throughout the body. In this study, analysis of laser ablation via a scanning galvanometer on a porcine colon tissue model is presented. A thermally damaged region (<85 μm) and fine depth control of ablation using the pulse energies 46 and 33 μJ are demonstrated. It is further demonstrated that such pulses suitable for precision porcine colon resection can be flexibly delivered via a hollow core negative curvature fibre (HC-NCF) and again ablation depth can be controlled with a thermally damaged region <85 μm. Ablation volumes are comparable to that of early stage lesions in the inner lining of the colon. This study concludes that the combination of ultrashort pulses and flexible fibre delivery via HC-NCF present a viable route to new minimally invasive surgical procedures. K E Y W O R D S negative curvature fibre, plasma-mediated ablation, porcine colon, thermal necrosis, ultrafast laser surgery

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Selective femtosecond laser ablation via two-photon fluorescence imaging through a multimode fiber

Cited by 41 publications

References 43 publications

Imaging hair cells through laser-ablated cochlear bone

Imaging hair cells through laser-ablated cochlear bone

Controlling spatiotemporal nonlinearities in multimode fibers with deep neural networks

Preclinical evaluation of porcine colon resection using hollow core negative curvature fibre delivered ultrafast laser pulses

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