Selective hydrophilic modification of Parylene C films: a new approach to cell micro-patterning for synthetic biology applications

Trantidou, Tatiana; Rao, Christopher; Barrett, Hayden P.; Camelliti, Patrizia; Pinto, Karina Zambone; Yacoub, Magdi H.; Athanasiou, Thanos; Toumazou, C.; Terracciano, Cesare; Prodromakis, Themis

doi:10.1088/1758-5082/6/2/025004

Cited by 39 publications

(38 citation statements)

References 41 publications

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“…13 Our group has previously developed a reliable and reproducible scaffolding technology using Parylene C thin films, which were micropatterned through selective modification of the material's inherent hydrophobicity using standard lithography and oxygen plasma. 2 These polymer scaffolds were able to align NRVM and significantly ( p < 0.05) improve their immature Ca 2+ transients at 0.5-2 Hz field stimulation. This scaffolding technology also enabled the production of structured NRVM cultures on planar Multi Electrode Arrays, which had an improved action potential conduction velocity and anisotropy.…”

Section: Introductionmentioning

confidence: 87%

“…2 In particular, we examined normalized fluorescent amplitude (f/f0), time to transient peak (Tp), time to 50% transient decay (T50), and time to 90% transient decay (T90), which were all significantly affected at 0.5, 1, and 2 Hz field stimulation.…”

Section: Calcium Cyclingmentioning

confidence: 99%

“…Among different approaches, cell patterning induces the alignment and elongation of immature cardiac myocytes, improving many morphological and physiological properties of cardiac tissue, such as sarcomere organization, 1 Ca 2+ handling, [1][2][3] gap junction protein (connexin43) expression, 4 and directionality of action potential propagation. [5][6][7][8][9] Several different methodologies have been developed for cardiac tissue engineering using neonatal rat ventricular myocytes (NRVM) 2,[5][6][7][8][9] and induced pluripotent stem cellderived cardiomyocytes.…”

Section: Introductionmentioning

confidence: 99%

“…[5][6][7][8][9] Several different methodologies have been developed for cardiac tissue engineering using neonatal rat ventricular myocytes (NRVM) 2,[5][6][7][8][9] and induced pluripotent stem cellderived cardiomyocytes. 1 These techniques include microcontact printing of substrates with extracellular matrix (ECM) compounds, 5 deposition of ECM proteins and cells through microfluidic channels, 10 microgrooved substrates, 1,5,6,8,9 nanotextured hydrogels, 11 and lithographically patterned substrates.…”

Section: Introductionmentioning

confidence: 99%

“…1 These techniques include microcontact printing of substrates with extracellular matrix (ECM) compounds, 5 deposition of ECM proteins and cells through microfluidic channels, 10 microgrooved substrates, 1,5,6,8,9 nanotextured hydrogels, 11 and lithographically patterned substrates. 2,7 Structured cardiomyocyte cultures have also been realized in three dimensions with the use of naturally or synthetically produced ECMs produced through electrospinning of aligned polymer nanofibers 12 and production of macroporous preformed scaffolds with complex biomimetic pore structures. 13 Our group has previously developed a reliable and reproducible scaffolding technology using Parylene C thin films, which were micropatterned through selective modification of the material's inherent hydrophobicity using standard lithography and oxygen plasma.…”

Section: Introductionmentioning

confidence: 99%

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Surface Chemistry and Microtopography of Parylene C Films Control the Morphology and Microtubule Density of Cardiac Myocytes

Trantidou

Humphrey

Poulet

et al. 2016

Tissue Engineering Part C: Methods

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Cell micropatterning has certainly proved to improve the morphological and physiological properties of cardiomyocytes in vitro; however, there is little knowledge on the single cell-scaffold interactions that influence the cells' development and differentiation in culture. In this study, we employ hydrophobic/hydrophilic micropatterned Parylene C thin films (2-10 mm) as cell microscaffolds that can control the morphology and microtubule density of neonatal rat ventricular myocytes (NRVM) by regulating their adhesion area on Parylene through a thickness-dependent hydrophobicity. Structured NRVM on thin films tend to bridge across the hydrophobic areas, demonstrating a more spread-out shape and sparser microtubule organization, while cells on thicker films adopt a cylindrical (in vivo-like) shape (contact angles at the level of the nucleus are 64.51°and 84.73°, respectively) and a significantly ( p < 0.05) denser microtubule structure. Ion scanning microscopy on NRVM revealed that cells on thicker membranes were significantly ( p < 0.05) smaller in volume, but more elongated. The cylindrical shape and a significantly denser microtubule structure indicate the ability to influence cardiomyocyte phenotype using patterning and manipulation of hydrophilicity. These combined bioengineering strategies are promising tools in the generation of more representative cardiomyocytes in culture.

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Section: Introductionmentioning

confidence: 87%

Section: Calcium Cyclingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Surface Chemistry and Microtopography of Parylene C Films Control the Morphology and Microtubule Density of Cardiac Myocytes

Trantidou

Humphrey

Poulet

et al. 2016

Tissue Engineering Part C: Methods

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Vapor‐Phase Synthesis of Poly(para‐xylylene): From Coatings to Porous and Hierarchical Materials

Hu,

Lee,

Ramli

et al. 2024

Adv Funct Materials

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Poly(para‐xylylene) (PPX) is a robust and biocompatible coating material that is widely used in various applications, including electronics, aerospace and defense materials, automotive materials, and biomaterials. In this progress report, recent developments in PPX technology ranging from the advancement of physical chemistry properties and structural properties for device integration to the transformation of 3D monolith materials of PPX with controls in outer and inner structures at the micro‐ and nanometer scales are highlighted. Based on emerging chemistry studies on [2.2]paracyclophanes, which are primarily used precursors to synthesize PPX via vapor deposition polymerization, functionalization, and the creation of a wide variety of functional PPX derivatives are demonstrated without resistance. Widely used as an interface coating material, PPX is processed to form integrated structural materials and has already been found to be useful in the market as part of electronic and medical implant products. Using a newly innovated transformation to fabricate PPX through templates (metal‐organic frameworks, liquid crystal, ice crystal), 3D monoliths, nanoscale particles, hierarchical and gradient interior structures, and dynamically transformable shapes at the nanoscale are demonstrated. A vast landscape of novel applications and device products is expected based on the already established R&D and market maturity of PPX.

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Photo‐Arbuzov Reactions as a Broadly Applicable Surface Modification Strategy

Plank,

Berardi,

Welle

et al. 2024

Adv Funct Materials

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Chemical vapor deposition (CVD) polymerization is a commonly used approach in surface chemistry, providing a substrate‐independent platform for bioactive surface functionalization strategies. This work investigates the Arbuzov reaction of halogenated polymer coatings readily available via CVD polymerization, using poly(4‐chloro‐para‐xylylene) (Parylene C) as a model substance. Postpolymerization modification of these coatings via catalyst‐free and UV‐induced Arbuzov reaction using phosphites results in phosphonate‐functionalized polymers. The combination of infrared reflection‐absorption spectroscopy (IRRAS), X‐ray photoelectron spectroscopy (XPS), and time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) provides detailed insights into the reaction progress. Time‐dependent studies suggest that the non‐polar phosphites penetrate deep into the CVD films and react with the polymer film. In addition, ToF‐SIMS, scanning electron microscopy (SEM), and atomic force microscopy (AFM) confirm spatial control of the reaction, resulting in localized chemical and topographical surface modification, recognizable by changes in interference color, fluorescence, and wettability. Preliminary 3D fluorescence spectroscopy investigations indicate tunable near‐infrared emission of these polymer films. This work is the first step toward generating multifunctional polymer coatings based on chemically modifiable, CVD polymers with potential applications in biomaterials, sensors, or optoelectronics.

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Selective hydrophilic modification of Parylene C films: a new approach to cell micro-patterning for synthetic biology applications

Abstract: Abstract. We demonstrate a simple, accurate and versatile method to manipulate Parylene C, a material widely known for its high biocompatibility, and transform it to a substrate that can effectively

Cited by 39 publications

References 41 publications

Surface Chemistry and Microtopography of Parylene C Films Control the Morphology and Microtubule Density of Cardiac Myocytes

Surface Chemistry and Microtopography of Parylene C Films Control the Morphology and Microtubule Density of Cardiac Myocytes

Vapor‐Phase Synthesis of Poly(para‐xylylene): From Coatings to Porous and Hierarchical Materials

Photo‐Arbuzov Reactions as a Broadly Applicable Surface Modification Strategy

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