Selective induction of putative iron transporters,<i>OPT8a</i> and <i>OPT8b</i>, in maize by mycorrhizal colonization

Kobae, Yoshihiro; Tomioka, Rie; Tanoi, Keitaro; Kobayashi, Natsuko I.; Ohmori, Yuki; Nishida, Sho; Fujiwara, Toru

doi:10.1080/00380768.2014.949854

Cited by 16 publications

(16 citation statements)

References 28 publications

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“…Although the main benefit of the AM association is an improved P status of the mycorrhizal plant, AM fungi also play a role in Fe nutrition of their host plants (Clark and Zeto, 1996;Liu et al, 2000;Chorianopoulou et al, 2015). Direct evidence of the capability of the extraradical mycelium (ERM) to take up Fe from the soil and to transfer it to the host plant comes from studies using 59 Fe as a tracer in compartments only accessible by the external hyphae (Caris et al, 1998;Kobae et al, 2014). Conversely, other studies have shown that AM fungi play a role in reducing Fe uptake when the soil concentration is high (Liu et al, 2000;Juwarkar and Jambhulkar, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Despite the fungal contribution to Fe uptake, expression of ZmYS1 encoding the transporter that mediates the uptake of PS-Fe(III) complexes from the rhizosphere was not affected in mycorrhizal roots. However, two oligopeptide transporter genes that were proposed to mediate the transport of Fe or Fe-complexes were highly induced in mycorrhizas (Kobae et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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The arbuscular mycorrhizal fungus Rhizophagus irregularis uses a reductive iron assimilation pathway for high‐affinity iron uptake

Tamayo

Knight

Valderas

et al. 2018

Environmental Microbiology

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Arbuscular mycorrhizal (AM) fungi can improve iron (Fe) acquisition of their host plants. Here, we report a characterization of two components of the high-affinity reductive Fe uptake system of Rhizophagus irregularis, the ferric reductase (RiFRE1) and the high affinity Fe permeases (RiFTR1-2). In the extraradical mycelia (ERM), Fe deficiency induced activation of a plasma membrane-localized ferric reductase, an enzyme that reduces Fe(III) sources to the more soluble Fe(II). Yeast mutant complementation assays showed that RiFRE1 encodes a functional ferric reductase and RiFTR1 an iron permease. In the heterologous system, RiFTR1 was expressed in the plasma membrane while RiFTR2 was expressed in the endomembranes. In the ERM, the highest expression levels of RiFTR1 were found in mycelia grown in media with 0.045 mM Fe, while RiFTR2 was upregulated under Fe-deficient conditions. RiFTR2 expression also increased in the intraradical mycelia (IRM) of maize plants grown without Fe. These data indicate that the Fe permease RiFTR1 plays a key role in Fe acquisition and that RiFTR2 is involved in Fe homeostasis under Fe-limiting conditions. RiFTR1 was highly expressed in the (IRM), which suggests that the maintenance of Fe homeostasis in the IRM might be essential for a successful symbiosis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The arbuscular mycorrhizal fungus Rhizophagus irregularis uses a reductive iron assimilation pathway for high‐affinity iron uptake

Tamayo

Knight

Valderas

et al. 2018

Environmental Microbiology

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“…Phenolics have also been identified in Fe-deficiency-induced root exudates ( Jin et al, 2014 ), although the precise constituents remain poorly understood. Additional complexities arise when plants are mycorrhizal; in maize, for instance, strong induction of two Fe transporters, OPT8a and OPT8b , bypassing Strategy II, by mycorrhizal colonization was documented ( Kobae et al, 2014 ). Thus, much needs to be learned about rhizospheric microbial partners and their roles in Fe acquisition.…”

Section: Physiological Changesmentioning

confidence: 99%

The Response of the Root Apex in Plant Adaptation to Iron Heterogeneity in Soil

Kronzucker

Shi

2016

Front. Plant Sci.

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Iron (Fe) is an essential micronutrient for plant growth and development, and is frequently limiting. By contrast, over-accumulation of Fe in plant tissues leads to toxicity. In soils, the distribution of Fe is highly heterogeneous. To cope with this heterogeneity, plant roots engage an array of adaptive responses to adjust their morphology and physiology. In this article, we review root morphological and physiological changes in response to low- and high-Fe conditions and highlight differences between these responses. We especially focus on the role of the root apex in dealing with the stresses resulting from Fe shortage and excess.

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“…irregularis (Kobae et al 2014b) was used to amplify the DNA fragments by PCR with the specific primers 5' -GCTCGAGATGTTACGGTATTCAAGTCCATTAGTTCA -3' and 5' -AGTCGACTTAAATAGCATCCGAGTTAGTCTCACCAC -3'. The amplified gene was ligated into pCR-Blunt II-TOPO vector (Life Technologies) and sequenced.…”

Section: Heterologous Expression Of Ringt In S Cerevisiaementioning

confidence: 99%

Up-regulation of genes involved in N-acetylglucosamine uptake and metabolism suggests a recycling mode of chitin in intraradical mycelium of arbuscular mycorrhizal fungi

et al. 2015

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Arbuscular mycorrhizal (AM) fungi colonize roots and form two kinds of mycelium, intraradical mycelium (IRM) and extraradical mycelium (ERM). Arbuscules are characteristic IRM structures that highly branch within host cells in order to mediate resource exchange between the symbionts. They are ephemeral structures and at the end of their life span, arbuscular branches collapse from the tip, fungal cytoplasm withdraws, and the whole arbuscule shrinks into fungal clumps. The exoskeleton of an arbuscule contains structured chitin, which is a polymer of N-acetylglucosamine (GlcNAc), whereas a collapsed arbuscule does not. The molecular mechanisms underlying the turnover of chitin in AM fungi remain unknown. Here, a GlcNAc transporter, RiNGT, was identified from the AM fungus Rhizophagus irregularis. Yeast mutants defective in endogenous GlcNAc uptake and expressing RiNGT took up (14)C-GlcNAc, and the optimum uptake was at acidic pH values (pH 4.0-4.5). The transcript levels of RiNGT in IRM in mycorrhizal Lotus japonicus roots were over 1000 times higher than those in ERM. GlcNAc-6-phosphate deacetylase (DAC1) and glucosamine-6-phosphate isomerase (NAG1) genes, which are related to the GlcNAc catabolism pathway, were also induced in IRM. Altogether, data suggest the existence of an enhanced recycling mode of GlcNAc in IRM of AM fungi.

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Selective induction of putative iron transporters,OPT8a and OPT8b, in maize by mycorrhizal colonization

Cited by 16 publications

References 28 publications

The arbuscular mycorrhizal fungus Rhizophagus irregularis uses a reductive iron assimilation pathway for high‐affinity iron uptake

The arbuscular mycorrhizal fungus Rhizophagus irregularis uses a reductive iron assimilation pathway for high‐affinity iron uptake

The Response of the Root Apex in Plant Adaptation to Iron Heterogeneity in Soil

Up-regulation of genes involved in N-acetylglucosamine uptake and metabolism suggests a recycling mode of chitin in intraradical mycelium of arbuscular mycorrhizal fungi

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