Selective inhibition by vasocortin of histamine release induced by dextran and concanavalin‐A from rat peritoneal cells

Abstract

Vasocortin, a glucocorticoid‐induced anti‐inflammatory protein, has been purified from the peritoneal lavage fluid of dexamethasone‐treated rats. Vasocortin inhibited the release of histamine from rat peritoneal cells stimulated by dextran or concanavalin A but did not alter the release induced by calcium ionophore A23187 or compound 48/80. This selective effect exhibited by vasocortin mimics the glucocorticoid inhibition of histamine release from rat mast cells.

In vitro inhibitory effect of rupatadine on histamine and TNF-α release from dispersed canine skin mast cells and the human mast cell line HMC-1

“…Anti-IgE antibody induces mem- brane-IgE-cross linking, leading to aggregation of the FceRI receptors causing MC degranulation and histamine release [18]. Con A is a lectin that may directly cross-link FceRI receptors and trigger the degranulation process [19,20]. Finally, calcium ionophore A23187 carries ions across the MC membrane, bypassing membrane receptors and calcium channels, leading to an increase of intracellular Ca 2+ levels that activates MC degranulation [21].…”

The astroglial-derived S100β protein stimulates the expression of nitric oxide synthase in rodent macrophages through p38 MAP kinase activation

“…Resident rat peritoneal macrophages were isolated according to the technique previously described by Carnuccio et al (1989). Both cell types were grown in Dulbecco Modified Eagle's Medium (DMEM) supplemented with 10% fetal calf serum, 2 mM glutamine, 100 U/mL penicillin, 100 mg/mL streptomycin at 37 -C in 5% CO 2 /95% air.…”

Selective inhibition by antiflamrnin‐2 of thromboxane B2 release from isolated and perfused guinea‐pig lung

“…This observation is consistent with previous results reported by us suggesting that another glucocorticold-induced protein (vasocortin) different from lipocortin 2 is responsible for the glucocorticoid inhibition of histamine release. 22 The release for the conflicting results reported by different authors on the biological activity of AF-2 is not clear. In fact, the mode of action of lipocortins and lipocortin-derived peptides as PLA2 inhibitors it is not yet understood.…”