Selective inhibition of membrane fusion events in echinoderm gametes and embryos by halenaquinol sulfate

Ikegami, Susumu; Kajiyama, Noboru; Ozaki, Yoshihiro; Myotoishi, Yuki; Miyashiro, Shigeyoshi; Takayama, Seiji; Kobayashi, Mariko; Kitagawa, Isao

doi:10.1016/0014-5793(92)80460-x

Cited by 11 publications

(4 citation statements)

References 11 publications

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“…In the process of fertilization, the egg and sperm find each other and fuse together to create a new individual with genetic potentials derived from both parents. There are few, if any, chemicals − that inhibit the fertilization events without affecting cell division. In the course of our continuing studies of bioactive metabolites from marine organisms, we have collected a sponge, Callyspongia truncata (Lindgren) (order Haplosclerida, family Callyspongiidae), off Sada-misaki, Ehime Prefecture, Japan, which showed antifertilization activities against starfish gametes but did not affect embryonic cell division during initial screening.…”

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confidence: 99%

Callyspongins A and B: Novel Polyacetylene Sulfates from the Marine Sponge Callyspongia truncata That Inhibit Fertilization of Starfish Gametes

Uno

Ohta

et al. 1996

J. Nat. Prod.

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Bioassay-guided fractionation of the fertilization-inhibitory constituents of the marine sponge Callyspongia truncata yielded two novel polyacetylene sulfates designated callyspongins A (1) and B (2). The structures were determined on the basis of spectroscopic data. Compounds 1 and 2 inhibited fertilization of starfish gametes with minimum inhibitory concentrations of 6.3 and 50 μM, respectively, but affected neither maturation of oocytes nor cell division of embryos. These findings indicate that the sulfate group plays an important role in specific inhibition of starfish fertilization.

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confidence: 99%

Callyspongins A and B: Novel Polyacetylene Sulfates from the Marine Sponge Callyspongia truncata That Inhibit Fertilization of Starfish Gametes

Uno

Ohta

et al. 1996

J. Nat. Prod.

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“…However, there are very few inhibitors capable of distinguishing between DNA polymerases a, 6 and E. In the present study, we show that halenaquinol sulfate (Fig. I), which is obtained from the sponge Xestospongia sapra and which is known to be an inhibitor of protein tyrosine kinase [5] as well as an inhibitor of cell membrane fusion [6], is a novel, selective inhibitor of DNA polymerases a and E with the competitive mode of inhibition with respect to dATP and dTTP. …”

Section: Introductionmentioning

confidence: 84%

Differential inhibition of eukaryotic DNA polymerases by halenaquinol sulfate, a p‐hydroquinone sulfate obtained from a marine sponge

et al. 1994

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Halenaquinol sulfate, a p-hydroquinone sulfate obtained from a marine sponge, inhibited the activity of eukaryotic DNA polymerases in varying degrees; the K, values for DNA polymerases a, /3, 6 and E were 1.3, 80, 17.5 and 2.0 ,uM, respectively, whereas it was less effective against E. co/i DNA polymerase I. The inhibition occurred competitively with each of dATP and dTTP, but non-competitively with dCTP, dGTP and the template DNA. Thus, halenaquinol sulfate is demonstrated to be a potential inhibitor of DNA polymerases a and E, and be a useful tool for analyzing the dNTP binding sites of DNA polymerases.

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“…Similarly to jaspisin, but differently from 10-epi-Axisonitrile-3 , there are other inhibitors of echinoderm fertilisation isolated from sponge, callyspongins A and B [170] and halenaquinol sulfate [171,172], that are able to inhibit sperm-egg fusion without affecting sperm motility [165]. Calyculin-A , originally derived from the marine sponge Discodermia calyx , is similar to okadaic acid in its potent inhibition of protein phosphatases and thus in its impact on reproduction [173,174].…”

Section: Introductionmentioning

confidence: 99%

Impact of Marine Drugs on Animal Reproductive Processes

Silvestre

Tosti

2009

Marine Drugs

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The discovery and description of bioactive substances from natural sources has been a research topic for the last 50 years. In this respect, marine animals have been used to extract many new compounds exerting different actions. Reproduction is a complex process whose main steps are the production and maturation of gametes, their activation, the fertilisation and the beginning of development. In the literature it has been shown that many substances extracted from marine organisms may have profound influence on the reproductive behaviour, function and reproductive strategies and survival of species. However, despite the central importance of reproduction and thus the maintenance of species, there are still few studies on how reproductive mechanisms are impacted by marine bioactive drugs. At present, studies in either marine and terrestrial animals have been particularly important in identifying what specific fine reproductive mechanisms are affected by marine-derived substances. In this review we describe the main steps of the biology of reproduction and the impact of substances from marine environment and organisms on the reproductive processes.

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Selective inhibition of membrane fusion events in echinoderm gametes and embryos by halenaquinol sulfate

Cited by 11 publications

References 11 publications

Callyspongins A and B: Novel Polyacetylene Sulfates from the Marine Sponge Callyspongia truncata That Inhibit Fertilization of Starfish Gametes

Callyspongins A and B: Novel Polyacetylene Sulfates from the Marine Sponge Callyspongia truncata That Inhibit Fertilization of Starfish Gametes

Differential inhibition of eukaryotic DNA polymerases by halenaquinol sulfate, a p‐hydroquinone sulfate obtained from a marine sponge

Impact of Marine Drugs on Animal Reproductive Processes

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