Selective isolation of bacterial cells within a microfluidic device using magnetic probe-based cell fishing

Micro-magnet arrays for specific single bacterial cell positioning

“…This improvement in cell array technology provides new perspectives for the development of innovative microsystem chips. We have recently demonstrated that micro-magnets could be integrated within microfluidic channels for future lab-on-chip applications [32,34]. This microfluidic integration can be exploited to elute non-target cells, while keeping the others at their individual trapping site.…”

“…Magnetic in situ hybridization (MISH) is a molecular biology technique that allows to identify specific cells through nucleic acid sequences (DNA or RNA) [34]. This method is based on the specific base pairing interaction between nucleic acids.…”

“…These micron scaled magnetic flux sources, requiring neither an external magnetic field nor a power supply, have been used to fabricate compact autonomous devices for the trapping of magnetic particles [32] and of magnetically-labeled eukaryotic cells [33]. So as to be adaptable to several applications in the field of microbiology, two different strategies were employed to label cells magnetically and specifically using (1) immunomagnetic labeling, based on antibody-antigen interactions and (2) magnetic in situ hybridization relying on a specific DNA sequence detection [34]. In both cases, we show that selective arraying of individual bacteria can be achieved using micromagnets of a size approaching that of bacteria.…”

Brain cells and neuronal networks: Encounters with controlled microenvironments

“…In this line, the magnetic-based technique where magnetically labeled cells can be efficiently captured thanks to the high magnetic field gradients generated by microflux sources is very promising. Its efficacy has been already demonstrated on bacteria [104] and on the mouse embryonic fibroblast cell line NIH/3T3 [105]. This large scale, multiplexed technique that can ultimately be pushed toward a single cell control is intrinsically transposable to other cells, e.g.…”

Magnetic nanoparticle DNA labeling for individual bacterial cell detection and recovery

“…The approach will benefit from very recent developments, including the availability of new and smaller nanoparticles for increasing the penetration capacity of the labeled DNA. Combined to adapted microfluidic systems (Pivetal et al, 2014) the approach could lead to the recovery of labeled cells for applying the single cell genomic concept leading to complete genome sequence reconstruction, a task impossible with the conventional metagenomics approach due to metagenome DNA complexity. a) DNA alone b) Nanoparticle-plasmid conjugate DNA strands Magnetic Nanoparticles Fig.…”