Selective laser melting porous metallic implants with immobilized silver nanoparticles kill and prevent biofilm formation by methicillin-resistant Staphylococcus aureus

Hengel, I.A.J. van; Riool, Martijn; Fratila‐Apachitei, Lidy E.; Witte-Bouma, Janneke; Farrell, Eric; Zadpoor, Amir A.; Zaat, Sebastian A. J.; Apachitei, I.

doi:10.1016/j.biomaterials.2017.02.030

Cited by 186 publications

(159 citation statements)

References 89 publications

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“…1 This may be because that NS-containing biomaterials could fight bacterial adhesion and clonal growth. 30,47 Although the bactericidal mechanisms of NS are not fully understood, 8 a possible explanation for the excellent antibacterial activities of PCL/NS1.0 and PCL/NS2.0 could be speculated based on previous reports and our results as follows: NSs have nanodimensions of 1-100 nm and large surface-to-volume ratio. The NS can interact with the sulfur-containing biomolecules of bacterial membrane protein and cause structural changes and damages to the cell membrane.…”

Section: Discussionmentioning

confidence: 72%

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Liu¹,

Luo²,

Wang³

et al. 2017

IJN

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Bacterial infection is a major hurdle to wound healing, and the overuse of antibiotics have led to global issue, such as emergence of multidrug-resistant bacteria, even “super bacteria”. On the contrary, nanosilver (NS) can kill bacteria without causing resistant bacterial strains. In this study, NS was simply generated in situ on the polycaprolactone (PCL) nanofibrous mesh using an environmentally benign and mussel-inspired dopamine (DA). Scanning electron microscopy showed that NS uniformly formed on the nanofibers of PCL mesh. Fourier transform infrared spectroscopy revealed the step-by-step preparation of pristine PCL mesh, including DA coating and NS formation, which were further verified by water contact angle changing from hydrophobic to hydrophilic. To optimize the NS dose, the antibacterial activity of PCL/NS against Staphylococcus aureus , Escherichia coli and Acinetobacter baumannii was detected by bacterial suspension assay, and the cytotoxicity of NS was evaluated using cellular morphology observation and Cell Counting Kit-8 (CCK8) assay. Then, inductively coupled plasma atomic emission spectrometry exhibited that the optimized PCL/NS had a safe and sustained silver release. Moreover, PCL/NS could effectively inhibit bacterial infection in an infectious murine full-thickness skin wound model. As demonstrated by the enhanced level of proliferating cell nuclear antigen (PCNA) in keratinocytes and longer length of neo-formed epidermis, PCL/NS accelerated wound healing by promoting re-epithelialization via enhancing keratinocyte proliferation in infectious wounds.

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Section: Discussionmentioning

confidence: 72%

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Liu¹,

Luo²,

Wang³

et al. 2017

IJN

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“…6,7 The large surface area of NPs leads to their amplified oxidation on the implant surface while the accompanying release of metallic ions induces strong bactericidal effects. 8 To incorporate antibacterial agents, the surface of the metallic implant needs to be biofunctionalized. While the modification of the surface chemistry and morphology may be used to prevent bacterial adhesion, the incorporation of antibacterial agents on the implant surface allows for actively targeting the bacteria residing in the adjacent tissues.…”

Section: Introductionmentioning

confidence: 99%

Self-defending additively manufactured bone implants bearing silver and copper nanoparticles

et al. 2020

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“…Ag + ions disrupt bacterial function through reaction with cell membrane . The antibacterial behavior of PEO coatings developed through the incorporation of Ag‐nanoparticles (AgNPs) on Titanium alloys have been studied by authors against Methicillin resistant Staphylococcus aureus (MRSA) bacteria …”

Section: Antibacterial Coatings Through Peomentioning

confidence: 99%

“…van Hengel et al developed AgNPs releasing antibacterial PEO coatings on porous Ti implants prepared through selective laser melting (SLM). The coating developed on porous implants prepared through SLM showed twofold larger zone of inhibition, fourfold increase in Ag ions release and almost tenfold reduction in colony‐forming units, when compared to PEO coated solid implants, while no signs of cytotoxicity were observed in human mesenchymal stem cells . Codeposition of Zn and AgNPs on Ti has been reported by Zhang et al, through the dispersion of AgNPs in an electrolyte containing zinc acetate.…”

Section: Antibacterial Coatings Through Peomentioning

confidence: 99%

“…Valve metals are light metals that have tendency to develop passive oxide film in the presence of oxygen, these include aluminum, magnesium, titanium, and zirconium . Apart from improving corrosion resistance and wear resistance, PEO has the ability to offer bioactive, biocompatible, and antibacterial surfaces through the incorporation of several particles or ions inside the porous surface. PEO offers several benefits including porous ceramic layer that can favor good osseointegration, good adhesion to the surface, mechanical fixation due to the bone cell growth inside the pores and ease of preparation .…”

Section: Introductionmentioning

confidence: 99%

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Surface modification of valve metals using plasma electrolytic oxidation for antibacterial applications: A review

Rizwan

Alias

Zaidi

et al. 2017

J Biomedical Materials Res

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Plasma electrolytic oxidation (PEO) is an advance technique to develop porous oxidation layer on light metals, primarily to enhance corrosion and wear resistance. The oxidation layer can also offer a wide variety of mechanical, biomedical, tribological, and antibacterial properties through the incorporation of several ions and particles. Due to the increasing need of antimicrobial surfaces for biomedical implants, antibacterial PEO coatings have been developed through the incorporation of antibacterial agents. Metallic nanoparticles that have been employed most widely as antibacterial agents are reported to demonstrate serious health and environmental threats. To overcome the current limitations of these coatings, there is a significant need to develop antibacterial surfaces that are not harmful for patient's health and environment. Attention of the readers has been directed to utilize bioactive glasses as antibacterial agents for PEO coatings. Bioactive glasses are well known for their excellent bioactivity, biocompatibility, and antibacterial character. PEO coatings incorporated with bioactive glasses can provide environment-friendly antimicrobial surfaces with exceptional bioactivity, biocompatibility, and osseointegration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 590-605, 2018.

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Selective laser melting porous metallic implants with immobilized silver nanoparticles kill and prevent biofilm formation by methicillin-resistant Staphylococcus aureus

Cited by 186 publications

References 89 publications

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Self-defending additively manufactured bone implants bearing silver and copper nanoparticles

Surface modification of valve metals using plasma electrolytic oxidation for antibacterial applications: A review

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