Selective MCA occlusion: A precise embolic stroke model

DiNapoli, Vincent; Rosen, Charles L.; Nagamine, Tomoaki; Crocco, Todd J.

doi:10.1016/j.jneumeth.2005.12.026

Cited by 54 publications

(73 citation statements)

References 17 publications

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“…We also confirmed in our study the potential of LDF for detecting embolic stroke model failure as already reported (Dinapoli et al, 2006;Dirnagl et al, 1989;Harada et al, 2005;Henninger et al, 2009;Woitzik and Schilling, 2002). We found that LDF identified adequately 4 of 5 failures in ES rats.…”

Section: Transcranial Duplex Sonography Like Laser Doppler Flowmetrysupporting

confidence: 91%

“…After a stable baseline was recorded for 10 min, stroke was induced and rCBF was assessed by LDF during 20 min immediately after ultrasound measurement at 1, 4 and 24 h after clot embolization. Based on previous publications in similar embolic models, we selected a stable ≤30% rCBF of pre-ischemic baseline at 1 h after embolization as the criteria for successful rICA occlusion after clot embolization and a rCBF threshold of 75% above baseline was chosen as indicative of recanalization (Dinapoli et al, 2006).…”

Section: Laser Doppler Flowmetry (Ldf)mentioning

confidence: 99%

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Transcranial duplex sonography for monitoring circle of Willis artery occlusion in a rat embolic stroke model

Lapergue

Deroide

Pocard

et al. 2011

Journal of Neuroscience Methods

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Section: Transcranial Duplex Sonography Like Laser Doppler Flowmetrysupporting

confidence: 91%

Section: Laser Doppler Flowmetry (Ldf)mentioning

confidence: 99%

Transcranial duplex sonography for monitoring circle of Willis artery occlusion in a rat embolic stroke model

Lapergue

Deroide

Pocard

et al. 2011

Journal of Neuroscience Methods

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“…after clot injection. A minimum initial reduction of 70% in the laser Doppler reading was considered a successful occlusion of middle cerebral artery perfusion territory [17]. Animals that did not show 70% reduction in the laser Doppler reading, were deemed to have had failed successful clot placement and were excluded from any further study.…”

Section: Methodsmentioning

confidence: 99%

Combination Therapy of Rosiglitazone, a Peroxisome Proliferator‐Activated Receptor‐γ Ligand, and NMDA Receptor Antagonist (MK‐801) on Experimental Embolic Stroke in Rats

Allahtavakoli

Shabanzadeh

Roohbakhsh

et al. 2007

Basic Clin Pharma Tox

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Peroxisome proliferator-activated receptor-γ (PPAR-γ ) agonists have been found to have potent anti-inflammatory actions and suggested as potential therapies for brain ischaemia. Glutamate is the most common excitatory neurotransmitter in the central nervous system and is released excessively during ischaemia. Stroke therapy will require combinations of drug classes, because no single drug class has yet been proven efficacious in human beings. The present study was conducted to assess whether N-methyl-d -aspartate (NMDA) receptor antagonist (MK-801) treatment can improve recovery from ischaemic brain injury and whether rosiglitazone, a PPAR-γ ligand, can increase its neuroprotective effect in an embolic model of stroke. Stroke was induced in rats by embolizing a preformed clot into the middle cerebral artery. Rosiglitazone (0.1 mg/kg, intraperitoneally) and MK-801 (0.1 mg/kg, intravenously) were injected immediately after embolization. Forty-eight hours later, the brains were removed, sectioned and stained with triphenyltetrazolum chloride and analysed by a commercial image processing software programme. Rosiglitazone and MK-801 alone or in combination decreased infarct volume by 49.16%, 50.26% and 81.32%, respectively (P < 0.001). Moreover, the combination therapy significantly decreased the infarct volume when compared to any drug used alone (P < 0.05). MK-801 reduced the brain oedema by 68% compared to the control group (P < 0.05), but rosiglitazone or combination did not show any significant effect. The drugs alone or in combination also demonstrated improved neurological function, but combination therapy was more effective on neurological deficits improving. Our data show that the combination of MK-801 and rosiglitazone is more neuroprotective in thromboembolic stroke than given alone; this effect perhaps represents a possible additive effect in the brain infarction.

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“…All rats underwent a transient ischemic stroke by occlusion of the right middle cerebral artery (MCAO) with tPA (5 mg/kg; iv) reperfusion (14). Young adult intact rats were confirmed to be in metestrus at the time of MCAO.…”

Section: Treatment Protocolmentioning

confidence: 99%

“…Previous studies from our laboratory underscore the importance of creating clinically relevant models of disease, which in the case of ischemic stroke includes the use of aged animals. Ischemic stroke in aged rats is characterized by increased ischemia/reperfusion injury, earlier disruptions of the blood-brain barrier, exacerbated neuronal degeneration, higher mortality, and reduced functional outcome (13)(14)(15).…”

mentioning

confidence: 99%

Worsened Outcome from Middle Cerebral Artery Occlusion in Aged Rats Receiving 17β-Estradiol

Leon¹,

Li²,

Huber

et al. 2012

Endocrinology

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Although estrogens are neuroprotective in young adult animal models of stroke, clinical trials demonstrate that estrogens increase the incidence and severity of stroke in aged women. We have previously shown that experimental stroke pathophysiology differs between young adult and aged rats. The aim of this study was to determine the effects of 17β-estradiol after middle cerebral artery occlusion and reperfusion in young adult and aged female rats. Focal embolic stroke was performed by middle cerebral artery occlusion with fibrin clot followed by reperfusion with i.v. human recombinant tissue plasminogen activator. Histological and functional outcomes were measured at 24 h after middle cerebral artery occlusion with fibrin clot. Aged rats treated with 17β-estradiol had significantly increased infarct volumes compared with placebo-treated aged rats. Young adult rats treated with 17β-estradiol had significantly decreased infarct volumes and improved functional outcome compared with ovariectomized young adult rats. Our results suggest that 17β-estradiol may act in an age-dependent manner in the postischemic rat brain. In young adult rats, it is neuroprotective; chronic treatment with 17β-estradiol during aging leads to worsened ischemic brain injury in aged female rats.

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Selective MCA occlusion: A precise embolic stroke model

Cited by 54 publications

References 17 publications

Transcranial duplex sonography for monitoring circle of Willis artery occlusion in a rat embolic stroke model

Transcranial duplex sonography for monitoring circle of Willis artery occlusion in a rat embolic stroke model

Combination Therapy of Rosiglitazone, a Peroxisome Proliferator‐Activated Receptor‐γ Ligand, and NMDA Receptor Antagonist (MK‐801) on Experimental Embolic Stroke in Rats

Worsened Outcome from Middle Cerebral Artery Occlusion in Aged Rats Receiving 17β-Estradiol

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