Selective Medium Containing Fosfomycin, Nalidixic Acid, and Culture Supernatant of Rbodococcus equi for Isolation of Corynebacterium pseudotuberculosis

Abstract

FNR medium containing fosfomycin, nalidixic acid, bovine blood and culture supernatant of Rhodococcus equi was prepared by the present authors, and the medium did not inhibit growth of Corynebacterium pseudotuberculosis but completely hampered the growth of Bacillus subtilis, Staphylococcus auyeus, and Escherichia coli. The culture supernatant of R. equi facilitated detection of suspected colonies of C.pseudotube~culosis due to synergistic hemolysis. Rate of isolation of the organisms (from the trachea, larynx and nasal cavity of 16 slaughtered sheep with caseous abscess in the lung) was higher with FNR, the selective medium, than with nonselective medium. The selective medium was thus found to be useful for isolation of C.pseudotuberculosis from sheep.:) Corresponding author. U.S.Copyright Clearance <:onrcrCode Sraccrncnt: 0931 -1793/91/3810-0743$02.50/0 ZHAO, HIRAMUNE, KIKUCHI, YANAGAWA, ITO, HATTA, SERIKAWA and YoSIKO OE Murdoch Univ., Australia 1980) the rate of isolation of C.pseudotuberculosis from the respiratory organs of sheep with caseous abscess in lung was only 56.7% with her NTP medium. This suggests that FNR medium facilitates isolation of the organisms by revealing synergistic hemolysis and inhibiting growth of bacteria other than C. pseudotuberculosis.

Synergistic haemolytic activity and its correlation to phospholipase D productivity by Corynebacteruim pseudotuberculosis Egyptian isolates from sheep and buffaloes

“…Microbiological examinations, followed by biochemical identification, were used as a gold standard to confirm infection with C. pseudotuberculosis . In brief, bacteriological cultures were made of pus specimens and the resultant C. pseudotuberculosis -resembling colonies that stained Gram-positive were tested further for biochemical properties (glucose fermentation, urease and catalase) (7, 31). Synergistic haemolysis with Rhodococcus equi ATCC 33701 and inhibition of ß -haemolysis by Staphylococcus aureus ATCC 25923 were also evaluated (7, 31).…”

“…Isolates were cultured initially into brain heart agar supplied with fosfomzein and nalidixic acid (31). …”

Evaluation of the synergistic haemolytic activity of phospholipase D produced by Corynebacteruim pseudotuberculosis

“…Microbiological examinations, followed by biochemical identification, were used as a gold standard to confirm infection with C. pseudotuberculosis. In brief, bacteriological cultures were made of pus specimens and the resultant C. pseudotuberculosis-resembling colonies that stained Gram-positive were tested further for biochemical properties (glucose fermentation, urease and catalase) (Eggleton, 1991, Zhao et al, 1991. Synergistic haemolysis with Rhodococcus equi ATCC 33701 and inhibition of ß-haemolysis by Staphylococcus aureus ATCC 25923 were also evaluated (Eggleton, 1991, Zhao et al, 1991.…”

“…The study was undertaken with 7 isolates recovered from buffaloes Selim et al 7307 infected with OSD and 7 isolates recovered from sheep infected with (CLA). Isolates were cultured initially into brain heart agar supplied with fosfomzein and nalidixic acid (Zhao et al, 1991). Biotypes were determined by conventional tests as described previously (Barakat et al, 1984) in addition to starch hydrolysis.…”