Selective mineral transport barriers at <i>Cuscuta</i>‐host infection sites

Förste, Frank; Mantouvalou, Ioanna; Kanngießer, Birgit; Stosnach, Hagen; Lachner, Lena Anna-Maria; Fischer, Karsten; Krause, Kirsten

doi:10.1111/ppl.13035

Cited by 15 publications

(16 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cuscuta reflexa , Cuscuta campestris, and Cuscuta platyloba were grown in a greenhouse on the host Pelargonium zonale under continuous illumination and a constant temperature of 21°C (Förste et al, 2020). The bacteria and binary T‐DNA‐containing vectors pRedRoot and XM82, respectively, were kindly contributed by Prof. Harro Bouwmeester (University of Amsterdam, Netherlands) ( A. rhizogenes ) and Prof. Tessa Burch‐Smith (University of Tennessee, USA) ( A. tumefaciens ) and are described in more detail in other studies (Limpens et al, 2004; Libiakova et al, 2018; Bobik et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

A highly efficient protocol for transforming Cuscuta reflexa based on artificially induced infection sites

2020

Self Cite

View full text Add to dashboard Cite

The parasitic plant genus Cuscuta is notoriously difficult to transform and to propagate or regenerate in vitro. With it being a substantial threat to many agroecosystems, techniques allowing functional analysis of gene products involved in host interaction and infection mechanisms are, however, in high demand. We set out to explore whether Agrobacterium‐mediated transformation of different plant parts can provide efficient alternatives to the currently scarce and inefficient protocols for transgene expression in Cuscuta. We used fluorescent protein genes on the T‐DNA as markers for transformation efficiency and transformation stability. As a result, we present a novel highly efficient transformation protocol for Cuscuta reflexa cells that exploits the propensity of the infection organ to take up and express transgenes with the T‐DNA. Both, Agrobacterium rhizogenes and Agrobacterium tumefaciens carrying binary transformation vectors with reporter fluorochromes yielded high numbers of transformation events. An overwhelming majority of transformed cells were observed in the cell layer below the adhesive disk’s epidermis, suggesting that these cells are particularly susceptible to infection. Cotransformation of these cells happens frequently when Agrobacterium strains carrying different constructs are applied together. Explants containing transformed tissue expressed the fluorescent markers in in vitro culture for several weeks, offering a future possibility for development of transformed cells into callus. These results are discussed with respect to the future potential of this technique and with respect to the special characteristics of the infection organ that may explain its competence to take up the foreign DNA.

show abstract

Section: Methodsmentioning

confidence: 99%

A highly efficient protocol for transforming Cuscuta reflexa based on artificially induced infection sites

2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cuscuta reflexa, Cuscuta campestris and Cuscuta platyloba were grown in a greenhouse on the host Pelargonium zonale under continuous illumination and a constant temperature of 21 °C (Förste et al, 2019). The bacteria and binary T-DNA-containing vectors pRedRoot and XM82, respectively, were kindly contributed by Prof. Harro Bouwmeester (University of Amsterdam, Netherlands) ( A. rhizogenes ) and Prof. Tessa Burch-Smith (University of Tennessee, USA) ( A. tumefaciens ) and are described in more detail in other studies (Limpens et al, 2004; Libiakova et al, 2018; Bobik et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

A highly efficient protocol for transformingCuscuta reflexabased on artificially induced infection sites

Lachner

Galstyan

Krause

2020

Preprint

Self Cite

View full text Add to dashboard Cite

A protocol that yields high numbers of transformed cells in the adhesive disks of Cuscuta reflexa 27 upon exposure to agrobacteria brings closer the vision of generating genetically modified 28 Cuscuta. 29 30 ABSTRACT 31 A current bottleneck in the functional analysis of the emerging parasitic model plant Cuscuta and 32 the exploitation of its recently sequenced genomes is the lack of efficient transformation tools. 33 Here, we describe the development of a novel highly efficient Agrobacterium-mediated 34 transformation protocol for Cuscuta reflexa based on the parasitic structure referred to as 35 adhesive disk. Both, Agrobacterium rhizogenes and Agrobacterium tumefaciens carrying binary 36 transformation vectors with reporter fluorochromes yielded high numbers of transformation 37 events. An overwhelming majority of transformed cells were observed in the cell layer below the 38 adhesive disk's epidermis, suggesting that these cells are particularly susceptible to infection. Co-39 transformation of these cells happens frequently when Agrobacterium strains carrying different 40 constructs are applied together. Explants containing transformed tissue expressed the fluorescent 41 markers in in vitro culture for several weeks, offering a possibility for development of 42 transformed cells into callus. 43 44 45 3 46 48 worldwide and infects a broad range of host plants. The parasite starts an attack by winding 49around the host stem. A multicellular feeding structure termed the haustorium, which can reach 50 2-3 mm in size in some species and originates from a secondary meristem in the cortex close to 51 the vascular bundles, develops within a few days and breaches the host tissue integrity using 52 mechanical pressure and enzymatic digestion of cell walls (Nagar et al., 1984; Johnsen et al., 53 2015; Kaiser et al., 2015). To make the penetration possible, a sucker-shaped attachment organ 54 provides the necessary counterforce. The development of this organ, termed "adhesive disk" or 55

show abstract

“…The haustorial connection between parasitic plants and their hosts is a site of extensive, bidirectional exchange of water, mineral nutrients, organic compounds and macromolecules [8], although a certain degree of selectivity exists [6,50]. The capability to tap into the nutrient supply of the host strongly depends on the lower water potential of the parasite, which in turn depends on the concentrations of particular compounds in the host tissue [6].…”

Section: Transmission Of Harmful Compounds From Host To Parasitementioning

confidence: 99%

“…Recently, selectivity for transport of mineral elements through the haustoria was demonstrated in Cuscuta reflexa Roxb. [50], which suggests that some parasites might be able to exclude harmful elements. The accumulation of harmful elements in the parasite seems to be dependent on host species and environmental conditions (Table 1).…”

Section: Transmission Of Harmful Compounds From Host To Parasitementioning

confidence: 99%

Plant Parasites under Pressure: Effects of Abiotic Stress on the Interactions between Parasitic Plants and Their Hosts

Zagorchev

Stöggl

Teofanova

et al. 2021

IJMS

View full text Add to dashboard Cite

Parasitic angiosperms, comprising a diverse group of flowering plants, are partially or fully dependent on their hosts to acquire water, mineral nutrients and organic compounds. Some have detrimental effects on agriculturally important crop plants. They are also intriguing model systems to study adaptive mechanisms required for the transition from an autotrophic to a heterotrophic metabolism. No less than any other plant, parasitic plants are affected by abiotic stress factors such as drought and changes in temperature, saline soils or contamination with metals or herbicides. These effects may be attributed to the direct influence of the stress, but also to diminished host availability and suitability. Although several studies on abiotic stress response of parasitic plants are available, still little is known about how abiotic factors affect host preferences, defense mechanisms of both hosts and parasites and the effects of combinations of abiotic and biotic stress experienced by the host plants. The latter effects are of specific interest as parasitic plants pose additional pressure on contemporary agriculture in times of climate change. This review summarizes the existing literature on abiotic stress response of parasitic plants, highlighting knowledge gaps and discussing perspectives for future research and potential agricultural applications.

show abstract

Selective mineral transport barriers at Cuscuta‐host infection sites

Cited by 15 publications

References 58 publications

A highly efficient protocol for transforming Cuscuta reflexa based on artificially induced infection sites

A highly efficient protocol for transforming Cuscuta reflexa based on artificially induced infection sites

A highly efficient protocol for transformingCuscuta reflexabased on artificially induced infection sites

Plant Parasites under Pressure: Effects of Abiotic Stress on the Interactions between Parasitic Plants and Their Hosts

Contact Info

Product

Resources

About