Selective removal of natural caries lesions from dentin and tooth occlusal surfaces using a diode-pumped Er:YAG laser

Jew, Jamison; Chan, Kenneth H.; Darling, Cynthia L.; Fried, Daniel

doi:10.1117/12.2256728

Cited by 6 publications

(6 citation statements)

References 19 publications

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“…Dentistry is constantly searching for treatments that cause less discomfort to patients. This is also important for dentists working in the context of the pandemic, which requires professional preventive and minimally invasive approaches to the management of caries ( 7 ).New technologies such as cavity preparation by oscillation (ultrasound) ( 8 ), lasers ( 9 ), ozone ( 10 ), and chemomechanical caries removal agents ( 11 ) have been used. Most of these methods involve a high cost and may lead to tooth hypersensitivity, contributing to painful sensations, especially in children.Chemomechanical caries removal is a non-invasive technique that aims to dissolve necrotic tissues, facilitating the removal of the tissue softened manually with blunt-tip instruments.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and dentin composition alterations promoted by different chemomechanical caries removal agents: A preliminary in vitro study

Guedes¹,

Bonvicini²,

Souza³

et al. 2021

J Clin Exp Dent

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Background The use of chemomechanical agents for caries removal has been indicated as a non-invasive treatment option; however, their possible deleterious effects on the dental-pulp complex have been insufficiently studied. This study assessed the direct cytotoxicity of two chemomechanical caries removal agents (Brix 3000™ - BX and Papacarie Duo™ - PD) on pulp cells from deciduous teeth, as well as to assess the morphology and chemical compositions of the dentin surface after the application of these materials. Material and Methods The cells were seeded (50,000 cells/cm²) in a culture medium (DMEM with 10% fetal bovine serum - FBS). After 24 hours, the BX and PD materials were added to 1:20, 1:100, and 1:1000 dilutions. Non-exposed cells were considered as the control group. The viability test (MTT), Trypan Blue assay (TB), and cell morphology (Scanning Electron Microscopy - SEM) were performed after 24 hours of agent application. For the SEM and chemical (energy-dispersive X-ray spectrometry - EDS) dentin evaluation, 0.3-mm-thick dentin discs were obtained and divided into control group (no treatment) and surfaces covered with 37% phosphoric acid, BX, or PD. Data were compared by one-way ANOVA and Tukey’s test ( p <0.05). Results Decreases in cell viability and numbers of viable cells were observed for both materials, at all dilutions, when compared with the control group ( p <0.05). The BX and PD materials did not cause visually perceptible changes, according to SEM, on the surfaces of dentin discs. The EDS analysis did not indicate a statistically significant difference in the levels of calcium (Ca) and phosphorus (P) between the materials and the control group ( p >0.05). Conclusions Both materials showed cytotoxicity when in direct contact with the pulp cells from deciduous teeth, and the BX material presented lower cytotoxicity than the PD material. Moreover, both materials did not significantly change the dentin composition. Key words: Cell culture, cytotoxicity, dental pulp, papacarie, primary teeth.

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Section: Introductionmentioning

confidence: 99%

Cytotoxicity and dentin composition alterations promoted by different chemomechanical caries removal agents: A preliminary in vitro study

Guedes¹,

Bonvicini²,

Souza³

et al. 2021

J Clin Exp Dent

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“…The main objective of caries removal is to remove the infected and necrotic tissues and microorganisms that cause inflammation and treatment failure. The ordinary caries treatment not always removes all of the microorganisms in the residual tissues (Jew et al 2017), as the bacteria are capable to diffuse into the dentinal tubules up to a depth of 1 mm, while the chemical disinfectants penetrate up to 130 μm inside the dentine (Gutknecht and Franzen 2004). This difference in depth of penetration between the invading bacteria and the disinfectant solution is responsible for the recurrent caries in many of the cases which can be observed in the conventional dental procedures.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial effect of metallic nanoparticles on Streptococcus mutans bacterial strain with or without diode laser (970 nm)

Sadony

Abozaid

2019

Bull Natl Res Cent

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Background: The main etiological agent for dental caries is Streptococcus mutans bacterial strain. Recently, metallic nanoparticles and diode laser have been introduced as a new method in bacterial decontamination. Nanoparticles have very small particles size that has greater surface area and exert their antimicrobial effect by interacting with the negatively charged bacterial cell wall, while lasers have become increasingly popular in dentistry due to their multiple uses, and one such important application is tooth disinfection. Objective: Present study was conducted to evaluate antimicrobial efficacy of silver (AgNPs) and gold nanoparticles (AuNPs) with and without diode laser (970 nm, 15 Hz, 100 mJ, 2 W, each cycle was 10 s and repeated 6 times with intervals of 5 s for each sample) irradiation against experimentally inoculated S. mutans bacteria in the samples. Material and methods: Thirty freshly a traumatically extracted premolars human tooth were collected, and cylindrical cavity in crowns with 4 mm diameter and 5 mm depth was prepared and inoculated with S. mutans for 24 h. The tooth were randomlly divided into equal expermintal groups (6 each): group 1, positive control group by S. mutans bacterial strain; group 2, irrigation with silver nanoparticles (AgNPs) for 3 min; group 3, irrigation with gold nanoparticles (AuNPs) for 3 min; group 4, 3 ml AgNPs + irradiation with diode laser for 60 s; and group 5, 3 ml AuNPs + irradiation with diode for 60 s. The specimens were collected from the teeth sample, and colony-forming units (CFU's) were observed by counting the bacteria colony on plates. Results: A significant difference was found among all the groups in comparison to the control group (p < 0.05). The greatest reduction in CFU's was observed in combination of AgNPs with diode laser group. Conclusions: The NPs in combination with diode laser irradiation has the potential to be used as dentine disinfectant.

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“…The specimen was taken using a small sterile microbrush scrubbed within the dentinal cavity bottom, in order to study the different microbial community and the percentage of microbial reduction after the treatments. This was done in order to avoid any dentin biopsy or surgical extraction of the tooth, excluding iatrogenic damages and the removal of healthy dentin together with infected, preserving a greater amount of tissue above the pulpal chamber [ 30 ]. A brief diagram in Figure 2 illustrates the treatment and sampling procedures for both the control (group A, CT) and intervention group (group B, Er:YAG therapy).…”

Section: Methodsmentioning

confidence: 99%

Use of the Er:YAG Laser in Conservative Dentistry: Evaluation of the Microbial Population in Carious Lesions

et al. 2021

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The aim of this study is to investigate the Erbium:Yttrio-Aluminum-Granate (Er:YAG) laser photothermal and mechanical effects on cariogenic species concentration and on the microbial load composition of therapeutic cavities, in order to evaluate the possible micro-organisms reduction and make a comparison with manual and rotating conventional therapy (CT). A clinical trial was designed, including adults with active deep carious lesions on permanent teeth who were divided into two groups, i.e., control group and intervention group treated with CT and Er:YAG therapy, respectively. Before and after any conservative treatment, two oral samples were collected using a small sterile microbrush scrubbed within the base of the dentinal cavity tissue. The percentage of reduction and the colony-forming units (CFUs) count after Er:YAG and conventional treatments were compared for total microorganisms, including Candida spp., Streptococcus spp., and Lactobacillus spp. The microbial reduction varied from 90.2% to 100% and was significantly observed for total microorganisms and Streptococcus spp. (p < 0.05). The Er:YAG laser shows the potential for clinical applications, especially with paediatric and complicated patients, thanks to its minimally invasive properties and its effect on the reduction of microbial load.

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Selective removal of natural caries lesions from dentin and tooth occlusal surfaces using a diode-pumped Er:YAG laser

Cited by 6 publications

References 19 publications

Cytotoxicity and dentin composition alterations promoted by different chemomechanical caries removal agents: A preliminary in vitro study

Cytotoxicity and dentin composition alterations promoted by different chemomechanical caries removal agents: A preliminary in vitro study

Antibacterial effect of metallic nanoparticles on Streptococcus mutans bacterial strain with or without diode laser (970 nm)

Use of the Er:YAG Laser in Conservative Dentistry: Evaluation of the Microbial Population in Carious Lesions

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