Selectively monitoring glutathione in human serum and growth-associated living cells using gold nanoclusters

Xie, Xiaoxue; Peng, Z.-S.; Hua, Xinyi; Wang, Zhifang; Deng, Keqin; Yang, Xiumei; Huang, Haowen

doi:10.1016/j.bios.2019.111829

Cited by 36 publications

(11 citation statements)

References 58 publications

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“…Unfortunately, such an ideal ratiometric GSH probe is not able to measure GSH in mitochondria, as there are no additional reactive sites available on the molecule for docking organelle-targeting motifs. Although many other fluorescence sensors for intracellular GSH were reported, − none of them is able to monitor GSH concentration in real time and in a reversible manner.…”

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confidence: 99%

Fully Reversible Ratiometric Nanosensors for Continuously Quantifying Mitochondrial Glutathione Concentration in Living Cells

et al. 2022

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Mitochondrial glutathione (mGSH) is both the cause of the oxidative damage and a mechanism for maintaining the redox homeostasis in mitochondria. To effectively measure mGSH dynamics in living cells, we have developed a new FRET-based nanosensor by immobilizing rhodamine B into dendritic mesoporous silica nanoparticles and installing GSH probes and mitochondria-targeting motifs onto the surface of nanoparticles. The result shows that these nanosensors show efficient FRET and a full reversibility and rapid response (<10 s) to GSH in the range of 0.5−20 mM, due to their unique nanostructure and well-overlapped spectra. The excellent photostability and low cytotoxicity make them an effective means for monitoring mGSH concentration in real time. When the mGSH nanosensors are used for quantitatively measuring mGSH variations under glucose deprivation stimulation in HeLa cells, they successfully prove themselves a useful tool for mitochondrial redox activity studies.

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confidence: 99%

Fully Reversible Ratiometric Nanosensors for Continuously Quantifying Mitochondrial Glutathione Concentration in Living Cells

et al. 2022

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“…All the microscope settings were kept consistent in each experiment. based on our previous work, 34 which was used as scaffold to anchor the aptamer in a 96-well plate. First, CM-Au NCs were added to a 96-well plate and placed in a vacuum oven to evaporate the solvent at 40 °C.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

De Novo Development of a Universal Biosensing Platform by Rapid Direct Native Protein Modification

Hua

Wang

et al. 2021

Anal. Chem.

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An innovative biosensing assay was developed for simplified, cost-effective, and sensitive detection. By rapid, direct treatment of target proteins with iron porphyrin (TPPFe) in situ, a carboxyl group of amino acid conjugates with an Fe atom of the TPPFe molecule, forming a stable protein complex. We have shown that this complex not only maintains the integrity and functions of original proteins but also acquires peroxidase activity that can turn TMB to a comparably visible signal like that in ELISA. This study is unique since such conversion is difficult to achieve with standard chemical modification or molecular biology methods. In addition, the proposed immunoassay is superior to traditional ELISA as it eliminates an expensive and complicated cross-linking process of an enzyme-labeled antibody. From a practical point of view, we extended this assay to rapid detection of clinically relevant proteins and glucose in blood samples. The results show that this simple immunoassay provides clinical diagnosis, food safety, and environmental monitoring in an easy-to-implement manner.

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“…Gold nanoclusters (Au NCs) with bright emission have been widely investigated in sensing, imaging, and antibacterial agents, etc. [ 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. In recent years, we found that the cationic polymer (hyperbranched polyethyleneimine, hPEI) can act as an effective template to produce fluorescent Au NCs and silver NCs [ 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Visualization of Polymer–Surfactant Interaction by Dual-Emissive Gold Nanocluster Labeling

Zheng

Zhang

et al. 2022

Biosensors

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Polymer-surfactant interaction decides the performance of corresponding complexes, making its rapid and intuitionistic visualization important for enhancing the performance of products and/or processing in related fields. In this study, the fluorescence visualization of the interaction between cationic hyperbranched polyethyleneimine and anionic sodium dodecyl sulfonate surfactant was realized by dual-emissive gold nanocluster labeling. The sensing mechanism was due to the interaction-induced polymer conformation change, which regulated the molecular structure and subsequent photoradiation process of the gold nanoclusters. All three inflection points of the interactions between the polymers and the surfactants were obtained by the change in fluorescence emission ratio of the designed dual-emissive gold nanoclusters. Moreover, these inflection points are verified by the hydrodynamic diameter and zeta potential measurements.

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Selectively monitoring glutathione in human serum and growth-associated living cells using gold nanoclusters

Cited by 36 publications

References 58 publications

Fully Reversible Ratiometric Nanosensors for Continuously Quantifying Mitochondrial Glutathione Concentration in Living Cells

Fully Reversible Ratiometric Nanosensors for Continuously Quantifying Mitochondrial Glutathione Concentration in Living Cells

De Novo Development of a Universal Biosensing Platform by Rapid Direct Native Protein Modification

Visualization of Polymer–Surfactant Interaction by Dual-Emissive Gold Nanocluster Labeling

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