Selectivity at a three-base bulge site in the DNA binding of ΔΔ-[{Ru(phen)2}2(μ-dppm)]4+ [dppm is 4,6-bis(2-pyridyl)pyrimidine; phen is 1,10-phenanthroline]

Morgan, Joy L.; Buck, Damian P.; Turley, Adam G.; Collins, J. Grant; Keene, F. Richard

doi:10.1007/s00775-006-0130-9

Cited by 23 publications

(10 citation statements)

References 40 publications

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“…292 Similar complexes to these have also shown bulge-binding specificity, which includes hairpin and cruciform structures; this preference may have applications for targeted gene expression modulation. [292][293][294][295][296][297] G-quadruplexes G-quadruplex DNA (QDNA) is a structure consisting of four guanine bases bound together in a Hoogsteen fashion that is stabilised by monovalent metal ions. The four bases can join from multiple strands or from the same strand, depending on the conformation (Fig.…”

Section: Cruciforms and Hairpinsmentioning

confidence: 99%

Metal complex interactions with DNA

2015

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Increasing numbers of DNA structures are being revealed using biophysical, spectroscopic and genomic methods. The diversity of transition metal complexes is also growing, as the unique contributions that transition metals bring to the overall structure of metal complexes depend on the various coordination numbers, geometries, physiologically relevant redox potentials, as well as kinetic and thermodynamic characteristics. The vast range of ligands that can be utilised must also be considered. Given this diversity, a variety of biological interactions is not unexpected. Specifically, interactions with negatively-charged DNA can arise due to covalent/coordinate or subtle non-coordinate interactions such as electrostatic attraction, groove binding and intercalation as well as combinations of all of these modes. The potential of metal complexes as therapeutic agents is but one aspect of their utility. Complexes, both new and old, are currently being utilised in conjunction with spectroscopic and biological techniques to probe the interactions of DNA and its many structural forms. Here we present a review of metal complex-DNA interactions in which several binding modes and DNA structural forms are explored.

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Section: Cruciforms and Hairpinsmentioning

confidence: 99%

Metal complex interactions with DNA

2015

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“…In this study we have employed a widely-used fluorescent intercalator displacement (FID) assay [13][14][15][16][17][18][19] to screen the binding of the flexibly-linked complexes to bulge-containing, and corresponding control, oligonucleotides. The technique is based upon the loss of fluorescence resulting from the displacement of an intercalating dye such as ethidium bromide (EthBr) or thiazole orange (TO) from a DNA sequence by the molecule of interest.…”

Section: Introductionmentioning

confidence: 99%

Dinuclear ruthenium(ii) complexes with flexible bridges as non-duplex DNA binding agents

et al. 2007

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The stereoisomers of a series of dinuclear ruthenium(ii) complexes [{Ru(phen)(2)}(2)(micro-BL)](4+) (phen = 1,10-phenanthroline) with flexible bridging ligands (BL) bb2 {1,2-bis[4(4'-methyl-2,2'-bipyridyl)]ethane}, bb5 {1,5-bis[4(4'-methyl-2,2'-bipyridyl)]pentane}, bb7 {1,7-bis[4(4'-methyl-2,2'-bipyridyl)]heptane}, and bb10 {1,10-bis[4(4'-methyl-2,2'-bipyridyl)]decane} have been synthesised. Their binding to a control dodecanucleotide, d(CCGGAATTCCGG)(2), and a tridecanucleotide, d(CCGAGAATTCCGG)(2), which contains a single adenine bulge have been studied using fluorescence displacement assays involving intercalating and groove-binding dyes, equilibrium dialysis and binding affinity chromatography. The fluorescence intercalator displacement (FID) assay indicated that LambdaLambda-[{Ru(phen)(2)}(2)(micro-bb7)](4+) had the greatest binding affinity with all the oligonucleotides, whereas an analogous fluorescence technique using a minor-groove binding dye, equilibrium dialysis and affinity binding chromatography showed that DeltaDelta-[{Ru(phen)(2)}(2)(micro-bb7)](4+) had the strongest binding. An (1)H NMR study of the binding of the DeltaDelta-enantiomer of [{Ru(phen)(2)}(2)(micro-bb7)](4+) to d(CCGAGAATTCCGG)(2) confirmed the selectivity of the metal complex for the bulge site and provided the basis for an energy-minimised binding model of the dinuclear ruthenium complex with the single adenine bulge containing trinucleotide. The binding model demonstrated the ability of the flexibly-linked complex to follow the curvature of the DNA minor groove.

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“…Each concentration of metal complex was prepared in triplicate. The excitation wavelength was 540 nm with excitation and emission bandwidths of 20 nm each [49].…”

Section: Fluorescence Spectroscopy and Ethidium Bromide Displacement mentioning

confidence: 99%

A [4+2] mixed ligand approach to ruthenium DNA metallointercalators [Ru(tpa)(N–N)](PF6)2 using a tris(2-pyridylmethyl)amine (tpa) capping ligand

Kraft

Bischof

Loos

et al. 2009

Journal of Inorganic Biochemistry

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Selectivity at a three-base bulge site in the DNA binding of ΔΔ-[{Ru(phen)2}2(μ-dppm)]4+ [dppm is 4,6-bis(2-pyridyl)pyrimidine; phen is 1,10-phenanthroline]

Cited by 23 publications

References 40 publications

Metal complex interactions with DNA

Metal complex interactions with DNA

Dinuclear ruthenium(ii) complexes with flexible bridges as non-duplex DNA binding agents

A [4+2] mixed ligand approach to ruthenium DNA metallointercalators [Ru(tpa)(N–N)](PF6)2 using a tris(2-pyridylmethyl)amine (tpa) capping ligand

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