Selectivity enhancement in capillary electrophoresis by means of two-dimensional separation or dual detection concepts

Beutner, Andrea; Herl, Thomas; Matysik, Frank‐Michael

doi:10.1016/j.aca.2018.11.042

Cited by 11 publications

(12 citation statements)

References 128 publications

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“…In addition to combinations of different separation principles, the separation selectivity can be partially improved by using complementary detection techniques such as UV‐vis spectrophotometry, fluorescence, contactless conductivity, electrochemical detection, and MS [157].…”

Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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The review provides a comprehensive overview of developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, microscale isolation, and physicochemical characterization of peptides from 2019 up to approximately the middle of 2021. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis, such as sample preparation, sorption suppression, EOF control, and detection, are presented. New developments in the individual CE and CEC methods are demonstrated and several types of their applications are shown. They include qualitative and quantitative analysis, determination in complex biomatrices, monitoring of chemical and enzymatic reactions and physicochemical changes, amino acid, sequence, and chiral analyses, and peptide mapping of proteins. In addition, micropreparative separations and determination of significant physicochemical parameters of peptides by CE and CEC methods are described.

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Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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“…There is a need for powerful analytical methods as the number of samples, the sample complexity, and the amount of substances that need to be analyzed is steadily increasing [1]. In recent years, CE was established as a potent separation technique due to its high separation efficiency, the simplicity of the instrumental setup, the small sample and reagent consumption, and its high separation speed [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…An ongoing trend is also the coupling of a separation technique like CE with more than one detector. These dual detection concepts (DDCs) are especially interesting when both detectors provide complementary information [1,6]. There are several DDCs for CE described in the literature [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Dead volume–free flow splitting in capillary electrophoresis

2022

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In recent years, several dual detection concepts (DDCs) for CE were developed, which consisted of at least one nondestructive detector. For these DDCs, a linear detector arrangement could be used, which is not possible when both detectors are destructive. To overcome this problem, we developed a concept for the splitting of the CE stream utilizing commercially available flow splitters (FSs) that allow the parallel positioning of two destructive detectors. In this proof-of-concept study, T-and Y-shaped FSs were characterized regarding their suitability for DDCs. To keep it simple, a UV detector (UV) and a C 4 D were used for the characterization. The model system consisted of an acetonitrile-based background electrolyte and the two model substances, (ferrocenylmethyl)trimethylammonium iodide and caffeine. CE hyphenated to a UV detector (CE-UV) measurements revealed that the split ratio was about 50% for both FSs. CE-C 4 D was used to evaluate the peak shape in front of and behind the FSs. These measurements showed that there was no significant peak broadening introduced by the FSs. Additionally, there were no changes in the LODs in front of and behind the FSs. Furthermore, the flexibility of the new FS approach allowed the usage of capillaries with different ids (25-75 µm) for injection and detection.

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“…LC‐MS is by far the most assessed analytical technique employed for EVOO phenolic fraction characterization; due to its high separation efficiency and short analysis time, capillary electrophoresis [16–18] has been used as well, even though information reported are quite meagre and limited. Spectrophotometric methods were also employed for total phenolic content as well as and various antioxidant tests, without providing any qualitative information about the phenolic compounds in the sample [19].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of matrix effect in one‐dimensional and comprehensive two‐dimensional liquid chromatography for the determination of the phenolic fraction in extra virgin olive oils

Arena

Cacciola

Rigano

et al. 2020

J of Separation Science

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Olea europaea, meaning “European olive,” is a small tree belonging to the family Oleaceae, occurring in the Mediterranean Basin. Olive oil is an essential component of a balanced diet because of its nutritional value. Among micronutrients, phenolic compounds did show important beneficial effects for human health. The majority of the research studies on the phenol content are carried out by liquid chromatography combined to photodiode array and/or mass spectrometry detection; however, because of matrix complexity, one‐dimensional liquid chromatography cannot be sometimes sufficient to obtain rewarding separations, requiring more advanced analytical techniques. In this work, comprehensive two‐dimensional liquid chromatography, incorporating RP‐Amide and C18 stationary phases, in the first and second dimension, respectively, both under reversed phase conditions, was investigated for the determination of the phenolic fraction in extra virgin olive oil samples. As far as detection is concerned, triple quadrupole mass spectrometry was employed under multi reaction monitoring mode offering superior selectivity and sensitivity. The reduction of matrix effects, when using comprehensive two‐dimensional liquid chromatography with respect to conventional one‐dimensional liquid chromatography, was assessed by comparing the slopes of calibration curves built from standard solutions and spiked olive oil samples.

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Selectivity enhancement in capillary electrophoresis by means of two-dimensional separation or dual detection concepts

Cited by 11 publications

References 128 publications

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Dead volume–free flow splitting in capillary electrophoresis

Evaluation of matrix effect in one‐dimensional and comprehensive two‐dimensional liquid chromatography for the determination of the phenolic fraction in extra virgin olive oils

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