Selenite protects <i><scp>C</scp>aenorhabditis elegans</i> from oxidative stress via <scp>DAF</scp>‐16 and <scp>TRXR</scp>‐1

Li, Wen-Hsuan; Shi, Yanhong; Chang, Chun‐Han; Huang, Chi‐Wei; Liao, Vivian Hsiu‐Chuan

doi:10.1002/mnfr.201300404

Cited by 29 publications

(38 citation statements)

References 72 publications

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“…Previous studies reported that trace amount of Se(IV) exerts multiple beneficial effects on development, reproduction, cholinergic signaling, neuroprotection, and oxidative stress resistance in C. elegans [37], [40], [41]. In the present study, the protective potential of Se(IV) on C. elegans against P. aeruginosa infection was further investigated.…”

Section: Discussionmentioning

confidence: 83%

“…This implies a unique role of C. elegans selenoprotein. A recent study indicated that TRXR-1 is involved in Se(IV) regulating oxidative stress resistance in C. elegans [37]. The role of TRXR-1 in Se(IV)-mediated immune responses in C. elegans requires further elucidation.…”

Section: Discussionmentioning

confidence: 99%

“…SAL105 cultures without or with Se(IV) (0.01 µM) were fed with OP50 or PA14, respectively, at 20°C for 24 h. The expressions of LYS-7 were directly measured by observing the reporter green fluorescent protein (GFP) fluorescence. Fluorescence in 20 nematodes randomly selected from each set of treatments was analyzed using microscopy [37]. At least three biological and three technical replicates were performed for each experiment.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequent to this treatment, 20 nematodes randomly selected from each set of treatments were mounted onto microscope slides coated with 2% agarose, anaesthetized with 2 mM sodium azide, and capped with coverslips. The subcellular SKN-1 distribution was analyzed by fluorescence microscopy [37]. Expression patterns of LD1 worms were classified into three categories (low, medium, and high) with respect to major localization of the SKN-1::GFP fusion protein in intestinal cells.…”

Section: Methodsmentioning

confidence: 99%

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Selenite Enhances Immune Response against Pseudomonas aeruginosa PA14 via SKN-1 in Caenorhabditis elegans

Chang

Huang

et al. 2014

PLoS ONE

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BackgroundSelenium (Se) is an important nutrient that carries out many biological processes including maintaining optimal immune function. Here, inorganic selenite (Se(IV)) was evaluated for its pathogen resistance and potential-associated factors in Caenorhabditis elegans. The immune effects of Se(IV) were investigated by examining the responses of C. elegans to Pseudomonas aerugonisa PA14 strain.Principal FindingsSe(IV)-treated C. elegans showed increased survival under PA14 infection compared with untreated controls. The significant pathogen resistance of Se(IV) on C. elegans might not be attributed to the effects of Se(IV) on PA14 as Se(IV) showed no effect on bacterial quorum-sensing and virulence factors of PA14. This study showed that Se(IV) enhanced the expression of a gene pivotal for the innate immunity in C. elegans. The study found that the pathogen-resistant phenotypes contributed by Se(IV) was absent from the skn-1 mutant worms. Moreover, Se(IV) influenced the subcellular distribution of SKN-1/Nrf in C. elegans upon PA14 infection. Furthermore, Se(IV) increased mRNA levels of SKN-1 target genes (gst-4 and gcs-1).ConclusionsThis study found evidence of Se(IV) protecting C. elegans against P. aeruginosa PA14 infection by exerting effects on the innate immunity of C. elegans that is likely mediated via regulation of a SKN-1-dependent signaling pathway.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Selenite Enhances Immune Response against Pseudomonas aeruginosa PA14 via SKN-1 in Caenorhabditis elegans

Chang

Huang

et al. 2014

PLoS ONE

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“…The organic form of selenium, N‐γ‐(L‐Glutamyl)‐L‐selenomethionine (Glu‐SeMet), was identified in garlic and as the primary metabolic product of SeMet. The benefits of selenium have been recognized for immune enhancement, anti‐inflammatory, anti‐cancer, anti‐depressive, neuro‐protective, cardio‐protective, and hepato‐protective abilities associated with increased oxidative stress condition . In addition, SeMet has been reported to decrease free radical generation, modulate antioxidative protein activities, and prevent DNA damage caused by oxidative stress .…”

Section: Introductionmentioning

confidence: 99%

N‐γ‐(L‐Glutamyl)‐L‐selenomethionine enhances stress resistance and ameliorates aging indicators via the selenoprotein TRXR‐1 in Caenorhabditis elegans

Chang

Liao

2017

Molecular Nutrition Food Res

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Tangeretin promotes lifespan associated with insulin/insulin‐like growth factor‐1 signaling pathway and heat resistance in Caenorhabditis elegans

et al. 2021

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Tangeretin is a polymethoxylated flavonoid naturally occurred in citrus fruits with many pharmacological activities, such as anti‐inflammatory, antiproliferative, and neuroprotective properties. A previous study reported that tangeretin‐enriched orange extract could prolong the lifespan in Caenorhabditis elegans. However, the antiaging effect of tangeretin remains uncertain. In this study, we used the model organism C. elegans to conduct a lifespan test, observed the aging‐related functional changes of nematodes, the fluorescence changes of stress‐related proteins (DAF‐16 and HSP‐16.2) and its response to stress assay, and monitored the effect of tangeretin on the mRNA expression levels. The results showed that tangeretin supplementation (30 and 100 μM) extended the mean lifespan, slowed aging‐related functional declines, and increased the resistance against heat‐shock stress. Furthermore, tangeretin upregulated the mRNA expression of daf‐16, hsp‐16.2, and hsp‐16.49, promoted the nuclear localization of DAF‐16, and enhanced the fluorescence intensity of HSP‐16.2, while it had no effect on the lifespan of daf‐2, age‐1, and daf‐16 mutants. The current findings suggest that tangeretin can significantly extend the lifespan and enhance heat stress tolerance in an insulin/insulin‐like growth factor signaling dependent manner.

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Selenite protects Caenorhabditis elegans from oxidative stress via DAF‐16 and TRXR‐1

Abstract: These findings suggest that the antioxidant effects of selenite in C. elegans are mediated via DAF-16 and TRXR-1.

Cited by 29 publications

References 72 publications

Selenite Enhances Immune Response against Pseudomonas aeruginosa PA14 via SKN-1 in Caenorhabditis elegans

Selenite Enhances Immune Response against Pseudomonas aeruginosa PA14 via SKN-1 in Caenorhabditis elegans

N‐γ‐(L‐Glutamyl)‐L‐selenomethionine enhances stress resistance and ameliorates aging indicators via the selenoprotein TRXR‐1 in Caenorhabditis elegans

Tangeretin promotes lifespan associated with insulin/insulin‐like growth factor‐1 signaling pathway and heat resistance in Caenorhabditis elegans

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