Selenium-dependent antitumor immunomodulating activity of polysaccharides from roots of A. membranaceus

Li, Shuang; Fuling, Bian; Yue, Ling; Jin, Hua; Hong, Zongguo; Gao, Shu

doi:10.1016/j.ijbiomac.2014.05.020

Cited by 49 publications

(20 citation statements)

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“…However, the exact mechanism is still unknown, the possible explanation is that some components of FZKA such as P. heterophylla, 32 A. membranaceus, 33 O. diffusa 34 and S. nigrum 35 have demonstrated anti-tumor activity in human tumor cells. In vitro study, we also found that the addition of FZKA and gefitinib to A549 (a cell line with low EGFR-TKI sensitivity) and H1650 (a cell line with EGFR-TKI resistance) cells resulted in down-regulation of p-EGFR and p-MAPK, suggesting the anti-proliferation effect on FZKA and gefitinib was acted through inhibiting the activation of EGFR and its downstream pathway Ras/Raf/MAPK (To be published).…”

Section: Discussionmentioning

confidence: 99%

“…We aim to determine whether gefitinib plus CHM could prolong the progression-free survival (PFS) or median survival time (MST) in patients with NSCLC than gefitinib alone. Methods: We retrospectively analyzed 159 non-small-cell lung cancer patients with the method of retrospective case-control study, matching factors included gender, age categories (30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62)(63)(64)(65)(66)(67)(68)(69)(70)(71)(72)(73)(74)(75)(76)(77)(78)(79), pathological stage (IIIB or IV), smoking status (never: <100 lifetime cigarettes, or ever: ≥100 lifetime cigarettes), pathology, and performance status. Among the 159 patients, 100 patients treated with gefitinib (250 mg/day orally) plus CHM (''Fuzheng Kang'ai'' decoction, a Chinese herbal medicine, 250 ml/bid/day orally), 59 patients treated with gefitinib (250 mg/day orally) only.…”

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confidence: 99%

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Effect of gefitinib plus Chinese herbal medicine (CHM) in patients with advanced non-small-cell lung cancer: A retrospective case–control study

Yang¹,

Wu²,

Long³

et al. 2014

Complementary Therapies in Medicine

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Effect of gefitinib plus Chinese herbal medicine (CHM) in patients with advanced non-small-cell lung cancer: A retrospective case–control study

Yang¹,

Wu²,

Long³

et al. 2014

Complementary Therapies in Medicine

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“…Compared to Se-free polysaccharides, Se-polysaccharides displayed a significantly stronger antioxidant activity in vivo and in vitro [ 18 ]. Recent studies demonstrated that Se-polysaccharides isolated from medicinal plants exert antitumor activity in human breast cancer cells [ 19 ] and murine H22 ascitic hepatoma cells and S180 sarcoma cells [ 20 ]. In our previous study, we successfully purified Se-enriched polysaccharides from Pyracantha fortuneana (Se-PFPs), which consisted of carbohydrate (93.7%), uronic acid (2.1%), and 3.7 μg/g of Se [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, we demonstrated that Se-PFPs could attenuate CCl 4 -induced liver injury in mice [ 21 ]. Although the antitumor activity of Se-polysaccharides has been reported in a couple of cancer types [ 19 , 20 ], the underlying molecular mechanisms have rarely been clarified.…”

Section: Introductionmentioning

confidence: 99%

Selenium-enriched polysaccharides from Pyracantha fortuneana (Se-PFPs) inhibit the growth and invasive potential of ovarian cancer cells through inhibiting β-catenin signaling

et al. 2016

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Polysaccharides from medicinal plants exert antitumor activity in many cancers. Our previous study demonstrated that polysaccharides extracted from the selenium-enriched Pyracantha fortuneana (Se-PFPs) showed antiproliferative effect in breast cancer cell line. This study aimed to investigate the antitumor effect of Se-PFPs in ovarian cancer cells in vitro and in vivo. Se-PFPs could decrease cell viability, induce apoptosis, and inhibit migratory and invasive potentials in HEY and SKOV3 cells. These findings are supported by reduced expression of cyclin D1, Bcl-2 and MMP-9, enhanced cleavage of PARP and caspase-3, elevated activity of caspase-3 and caspase-9, and EMT (epithelial to mesenchymal transition) inhibition (elevated expression of E-cadherin and cytokeratin 19, and reduced expression of N-cadherin, vimentin, ZEB1 and ZEB2). Moreover, Se-PFPs inhibited xenografted tumor growth through inhibiting cell proliferation and inducing cell apoptosis. More importantly, Se-PFPs significantly reduced cytoplasmic β-catenin particularly nuclear β-catenin expression but increased β-catenin phosphorylation in a GSK-3β-dependent mechanism. Furthermore, β-catenin knockdown exerted similar effects on cell proliferation and invasion as seen in Se-PFPs-treated cells, while β-catenin overexpression neutralized the inhibitory effects of Se-PFPs on cell proliferation and invasion. Take together,Se-PFPs exert antitumor activity through inhibiting cell proliferation, migration, invasion and EMT, and inducing cell apoptosis. These effects are achieved by the inhibition of β-catenin signaling. Thus Se-PFPs can be used as potential therapeutic agents in the prevention and treatment of ovarian cancer.

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“…Polysaccharides from plants have been evaluated for their anti-tumor activities, in which the direct activities included induction of apoptosis of tumor cells, arrest of its cell cycle and inhibition of its invasion, adhesion and metastasis while the indirect activities included enhancement of the immune protection [32,33,34]. Recent studies have indicated that Se-containing polysaccharides provide the effects of reducing oxidative stress and antitumor immunomodulation [35,36]. Our previous research reported that polysaccharides derived from Se-enriched P. fortuneana had hepatoprotective effects [23,24,25].…”

Section: Introductionmentioning

confidence: 99%

Antimutagenic Effects of Selenium-Enriched Polysaccharides from Pyracantha fortuneana through Suppression of Cytochrome P450 1A Subfamily in the Mouse Liver

Peng

Guo

et al. 2016

Molecules

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Both selenium (Se) and polysaccharides from Pyracantha fortuneana (Maxim.) Li (PFPs) (P. fortuneana) have been reported to possess antioxidative and immuno-protective activities. Whether or not Se-containing polysaccharides (Se-PFPs) have synergistic effect of Se and polysaccharides on enhancing the antioxidant and immune activities remains to be determined. We previously reported that polysaccharides isolated from Se-enriched P. fortuneana (Se-PFPs) possessed hepatoprotective effects. However, it is not clear whether or not they have anti-mutagenic effects. In the present study, we compared and evaluated anti-mutagenic effects of Se-PFPs at three concentrations (1.35, 2.7 and 5.4 g/kg body weight) with those of PFPs, Se alone or Se + PFPs in mice using micronucleus assay in bone marrow and peripheral blood as well as mitomycin C-induced chromosomal aberrations in mouse testicular cells. We also elucidated the underlying mechanism. Our results demonstrated that Se-PFPs inhibited cyclophosphamide (CP)-induced micronucleus formation in both bone marrow and peripheral blood, enhanced the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in mouse liver, and reduced the activity and expression of cytochrome P450 1A (CYP4501A) in mouse liver in a dose-dependent manner. In addition, we found that the anti-mutagenic potential of Se-PFPs was higher than those of PFPs, Se alone or Se + PFPs at the same level. These results suggest that the anti-mutagenic potential of Se-PFPs may be mediated through the inhibition of the activity and expression of CYP4501A. This study indicates that application of Se-PFPs may provide an alternative strategy for cancer therapy by targeting CYP1A family.

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Selenium-dependent antitumor immunomodulating activity of polysaccharides from roots of A. membranaceus

Cited by 49 publications

References 45 publications

Effect of gefitinib plus Chinese herbal medicine (CHM) in patients with advanced non-small-cell lung cancer: A retrospective case–control study

Effect of gefitinib plus Chinese herbal medicine (CHM) in patients with advanced non-small-cell lung cancer: A retrospective case–control study

Selenium-enriched polysaccharides from Pyracantha fortuneana (Se-PFPs) inhibit the growth and invasive potential of ovarian cancer cells through inhibiting β-catenin signaling

Antimutagenic Effects of Selenium-Enriched Polysaccharides from Pyracantha fortuneana through Suppression of Cytochrome P450 1A Subfamily in the Mouse Liver

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