Selenium sensitizes MCF-7 breast cancer cells to doxorubicin-induced apoptosis through modulation of phospho-Akt and its downstream substrates

Li, Song; Zhou, Yunfei; Wang, Ruiwen; Zhang, Haitao; Dong, Yan; Ip, Clement

doi:10.1158/1535-7163.mct-06-0643

Cited by 109 publications

(90 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Synergistic tumor growth inhibition and reduced toxicity were shown by combination of organic selenium with the topoisomerase I poison irinotecan or doxorubicin in tumorbearing mice inoculated with cancer cells sensitive and resistant to irinotecan (19,20). Similar reports of synergy between selenium and chemotherapeutic drugs were recently reported in breast cancer (20).…”

Section: Introductionsupporting

confidence: 70%

Methylseleninic acid synergizes with tamoxifen to induce caspase-mediated apoptosis in breast cancer cells

Carrier

Rowan

2008

Molecular Cancer Therapeutics

View full text Add to dashboard Cite

Tamoxifen has efficacy as a breast cancer therapy and chemoprevention agent. However, toxicity and resistance to tamoxifen limit its clinical application. There is an urgent need to develop compounds that may be combined with tamoxifen to improve efficacy and overcome toxicity and resistance. We showed previously that the organoselenium compound methylseleninic acid (MSA) increased the growth-inhibitory effect of tamoxifen and reversed tamoxifen resistance in breast cancer cells. In this study, we examined the mechanism for induction of apoptosis by MSA combined with tamoxifen in tamoxifen-sensitive and tamoxifen-resistant breast cancer cells. 4-hydroxytamoxifen (TAM; 10 À7 mol/L) alone resulted in cell cycle arrest but no apoptosis, whereas MSA alone (10 Mmol/L) induced apoptosis in tamoxifen-sensitive cells. Combination of MSA with TAM resulted in a synergistic apoptosis in both tamoxifen-sensitive and tamoxifen-resistant breast cancer cells compared with either agent alone. MSA and MSA combined with TAM induced apoptosis through the intrinsic, mitochondrial apoptotic pathway. MSA induced a sequential activation of caspase-9 and then caspase-8. These results indicate that the growth inhibition synergy and reversal of tamoxifen resistance by combination of selenium with tamoxifen occurs via a tamoxifen-induced cell cycle arrest, allowing more cells to enter the intrinsic apoptotic pathway elicited by selenium.

show abstract

Section: Introductionsupporting

confidence: 70%

Methylseleninic acid synergizes with tamoxifen to induce caspase-mediated apoptosis in breast cancer cells

Carrier

Rowan

2008

Molecular Cancer Therapeutics

View full text Add to dashboard Cite

show abstract

“…Although flow cytometry analysis of Annexin V and PI double staining is a wellaccepted assay to determine apoptotic cell death, this method is not suitable for cells treated with DOX as it emits a very strong and broad-band fluorescence that interferes with the assay (16). Considering the afore-mentioned problem, we used trypan blue staining instead to quantify cell death caused by ASA, DOX, or the combination.…”

Section: Asa Promotes Dox-induced Cell Death In Vitromentioning

confidence: 99%

Aspirin enhances doxorubicin-induced apoptosis and reduces tumor growth in human hepatocellular carcinoma cells in vitro and in vivo

et al. 2012

View full text Add to dashboard Cite

Abstract. Combined therapy with multiple drugs is a common practice in the treatment of cancer, which can achieve better therapeutic effects than a single drug, and can reduce the side effects as well as drug resistance. This study aimed to determine whether aspirin (ASA) shows synergism with doxorubicin (DOX) in HepG2 human hepatocellular carcinoma cells in vitro and in a HepG2 cell xenograft model in BALB/c nude mice. When treated in combination, DOX (0.25 nmol/ ml) and ASA (5 µmol/ml) produced strong synergy in growth inhibition, cell cycle arrest and importantly, apoptosis in vitro in comparison to single treatments. Moreover, ASA (100 mg/ kg/day orally) and DOX (1.2 mg/kg biweekly ip) induced synergistic antitumor activity in the HepG2 cell xenograft model in nude mice. Therefore, the combination of ASA and DOX could be used as a novel combination regimen which provides a strong anticancer synergy in the treatment of hepatocellular carcinoma.

show abstract

“…Apoptosis has been described as one of the more important mechanisms for the anticancer action of selenium-based compounds. [35][36][37] Although Valdiglesias et al 38 reported that the induction of apoptosis by Seleniumbased compounds may be related to changes in the cell cycle, Suchocki et al 20 found that free selol did not overset the cell cycle in human leukemia cells.…”

Section: Discussionmentioning

confidence: 99%

Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy

Estevanato

Silva²,

Falqueiro³

et al. 2012

IJN

View full text Add to dashboard Cite

Antitumor activities have been described in selol, a hydrophobic mixture of molecules containing selenium in their structure, and also in maghemite magnetic nanoparticles (MNPs). Both selol and MNPs were co-encapsulated within poly(lactic-co-glycolic acid) (PLGA) nanocapsules for therapeutic purposes. The PLGA-nanocapsules loaded with MNPs and selol were labeled MSE-NC and characterized by transmission and scanning electron microscopy, electrophoretic mobility, photon correlation spectroscopy, presenting a monodisperse profile, and positive charge. The antitumor effect of MSE-NC was evaluated using normal (MCF-10A) and neoplastic (4T1 and MCF-7) breast cell lines. Nanocapsules containing only MNPs or selol were used as control. MTT assay showed that the cytotoxicity induced by MSE-NC was dose and time dependent. Normal cells were less affected than tumor cells. Cell death occurred mainly by apoptosis. Further exposure of MSE-NC treated neoplastic breast cells to an alternating magnetic field increased the antitumor effect of MSE-NC. It was concluded that selol-loaded magnetic PLGA-nanocapsules (MSE-NC) represent an effective magnetic material platform to promote magnetohyperthermia and thus a potential system for antitumor therapy.

show abstract

Selenium sensitizes MCF-7 breast cancer cells to doxorubicin-induced apoptosis through modulation of phospho-Akt and its downstream substrates

Abstract: Doxorubicin is an effective drug against breast cancer.

Cited by 109 publications

References 23 publications

Methylseleninic acid synergizes with tamoxifen to induce caspase-mediated apoptosis in breast cancer cells

Methylseleninic acid synergizes with tamoxifen to induce caspase-mediated apoptosis in breast cancer cells

Aspirin enhances doxorubicin-induced apoptosis and reduces tumor growth in human hepatocellular carcinoma cells in vitro and in vivo

Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy

Contact Info

Product

Resources

About