Selenoprotein H controls cell cycle progression and proliferation of human colorectal cancer cells

Bertz, Martin; Kuhn, Katrin Gaardbo; Koeberle, Solveigh C.; Müller, Mike F.; Hoelzer, Doerte; Thies, Karolin; Deubel, Stefanie; Thierbach, René; Kipp, Anna P.

doi:10.1016/j.freeradbiomed.2018.01.010

Cited by 48 publications

(29 citation statements)

References 43 publications

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“…Unlike GPx2, TXNRD1 and SELENOF, knockdown of SELENOH improves cell proliferation in vitro and promotes tumor growth in vivo, indicating that SELENOH may suppress tumor progression (79)(80)(81)(82). SELENOH affect cell cycle through inhibition of G1/S transition by modulating p21 and CCNE1 expression (83,84). The 15-kDa selenoprotein (Sep15) can also contribute to cancer progression (85)(86)(87)(88)(89).…”

Section: Resultsmentioning

confidence: 99%

Association of Selenium and Risk of Esophageal Cancer: A Review

Younesian

Hosseinzadeh

et al. 2020

mljgoums

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Esophageal cancer (EC) is one of the most common types of cancer, especially in Asia. Esophageal squamous cell cancer (ESCC) is the most important histological subtype of EC, which accounts for 90% of all EC cases worldwide. ESCC is highly prevalent in Turkey, Iran, Kazakhstan and northern and central parts of China. Selenium is an essential micronutrient that is required for cellular functioning and synthesis of several selenoproteins. It also modulates the antioxidant defense system, cell cycle and apoptosis. This article reviews the most important molecular mechanisms of EC and investigates the association between selenium level and incidence of EC in high-risk areas.

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Section: Resultsmentioning

confidence: 99%

Association of Selenium and Risk of Esophageal Cancer: A Review

Younesian

Hosseinzadeh

et al. 2020

mljgoums

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“…Some plant proteomes were also found to possess a few unknown or unspecified amino acids, Trifolium pratense (76). At least six species were found to possess a J (Xle) amino acid.…”

Section: Plants Possess Selenocysteine (Sec) and Other Novel Amino Acidsmentioning

confidence: 99%

The Molecular Mass and Isoelectric Point of Plant Proteomes

Mohanta

Khan

Hashem

et al. 2019

Preprint

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Background Cell contain diverse array of proteins with different molecular weight and isoelectric point (pI). The molecular weight and pI of protein play important role in determining the molecular biochemical function. Therefore, it was important to understand the detail regarding the molecular weight and pI of the plant proteins. Results A proteome-wide analysis of plant proteomes from 145 species revealed a pI range of 1.99 (epsin) to 13.96 (hypothetical protein). The spectrum of molecular mass of the plant proteins varied from 0.54 to 2236.8 kDa. A putative Type-I polyketide synthase (22244 amino acids) in Volvox carteri was found to be the largest protein in the plant kingdom. However, Type-I polyketide synthase was not found in higher plant species. Titin (806.46 kDa) and misin/midasin (730.02 kDa) were the largest proteins identified in higher plant species. The pI and molecular weight of the plant proteins showed a trimodal distribution. An acidic pI (56.44% of proteins) was found to be predominant over a basic pI (43.34% of proteins) and the abundance of acidic pI proteins was higher in unicellular algae species relative to multicellular higher plants. In contrast, the seaweed, Porphyra umbilicalis, possesses a higher proportion of basic pI proteins (70.09%). Plant proteomes were also found to contain selenocysteine (Sec), amino acid that was found only in lower eukaryotic aquatic plant lineage. Amino acid composition analysis showed Leu was high and Trp was low abundant amino acids in the plant proteome. Additionally, the plant proteomes also possess ambiguous amino acids Xaa (unknown), Asx (asparagine or aspartic acid), Glx (glutamine or glutamic acid), and Xle (leucine or isoleucine) as well. Conclusion The diverse molecular weight and isoelectric point range of plant proteome will be helpful to understand their biochemical and functional aspects. The presence of selenocysteine proteins in lower eukaryotic organism is of interest and their expression in higher plant system can help us to understand their functional role.

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“…The selenium is found in the active site of selenoproteins, more specifically involved in redox reactions [71]. In human, selenoprotein H regulates cell cycle progression and proliferation of human colorectal cancer cells [76]. Selenoprotein K enhances protein palmitoylation [77,78], selenoprotein T attenuates the development of left ventricular dysfunction after myocardial infraction in rats [79].…”

Section: Plants Possess Selenocysteine (Sec) and Other Novel Amino Acidsmentioning

confidence: 99%

“…Among the studied plant species, ten were found to contain amino acid B (Asx) that codes for the ambiguous amino acid Asn or Asp that is translated as Asp. Species that were found to possess an Asx amino acid included Arachis duranensis (1), Brachypodium stacei (40), Dichanthelium oligosanthes (20), Dunaliella salina (31), Glycine max (1), Malus domestica (4080), Momordica charantia (98), Nelumbo nucifera (64), Prunus persica (1), and Trifolium pratense (76). At least six species were found to possess a J (Xle) amino acid.…”

Section: Plants Possess Selenocysteine (Sec) and Other Novel Amino Acidsmentioning

confidence: 99%

The Molecular Mass and Isoelectric Point of Plant Proteomes

Mohanta

Khan

Hashem

et al. 2019

Preprint

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12A proteomic analysis of proteomes from 145 plant species revealed a pI range of 1.99 (epsin) 13 to 13.96 (hypothetical protein). The molecular mass of the plant proteins ranged from 0.54 to 14 2236.8 kDa. A putative Type-I polyketide synthase (22244 amino acids) in Volvox carteri was 15 found to be the largest protein in the plant kingdom and was not found in higher plant species. 16 Titin (806.46 kDa) and misin/midasin (730.02 kDa) were the largest proteins identified in 17 higher plant species. The pI and molecular weight of the plant proteome exhibited a trimodal 18 distribution. An acidic pI (56.44% of proteins) was found to be predominant over a basic pI 19 (43.34% of proteins) and the abundance of acidic pI proteins was higher in unicellular algae 20 species relative to multicellular higher plants. In contrast, the seaweed, Porphyra umbilicalis, 21 possesses a higher proportion of basic pI proteins (70.09%). Plant proteomes were also found 22 to contain the amino acid, selenocysteine (Sec), which is the first report of the presence of this 23 amino acid in plants. Additionally, plant proteomes also possess ambiguous amino acids Xaa 24 (unknown), Asx (asparagine or aspartic acid), Glx (glutamine or glutamic acid), and Xle 25 (leucine or isoleucine) as well.26 27 Pyrrolysine 29 30 31 32 33The isoelectric or isoionic point of a protein is the pH at which a protein carries no net electrical 34 charge and hence is considered neutral 1-4 . The zwitterion form of a protein becomes dominant 35 at neutral pH. The pI of polypeptides is largely dependent on the dissociation constant of the 36 ionisable groups 5 . The major ionisable groups present in the amino acids are arginine, 37 aspartate, cysteine, histidine, glutamate, lysine, and glutamate, where they play a major role in 38 determining the pI of a protein 6-8 . Co-translational and post-translational modifications of a 39 protein, however, can also play a significant role in determining the pI of a protein 9,10 . The 40 exposure of charged residues to the solvents, hydrogen bonds (diploe interactions) and 41 dehydration also impact the pI of a protein 11,12 . The inherent pI of protein, however, is 42 primarily based on its native protein sequence. The pI of a protein is crucial to understanding 43 its biochemical function and thus determining pI is an essential aspect of proteomic studies. 44During electrophoresis, the direction of movement of a protein in a gel or other matrix depends 45 its'pI, hence numerous proteins can be separated based on their pI [13][14][15][16] . Given the impact of 46 post-translational modifications and other biochemical alterations (phosphorylation, 47 49 9,17,18. Nonetheless, an estimated isoelectric point is highly important and a commonly 50 identified parameter. 51Several studies have been conducted to understand the pI of proteins/polypeptides 3,19-21 . These 52 studies have been mainly based on animal, bacteria, and virus models and databases containing 53 the pI of experimentally verified proteins. None of these da...

show abstract

Selenoprotein H controls cell cycle progression and proliferation of human colorectal cancer cells

Cited by 48 publications

References 43 publications

Association of Selenium and Risk of Esophageal Cancer: A Review

Association of Selenium and Risk of Esophageal Cancer: A Review

The Molecular Mass and Isoelectric Point of Plant Proteomes

The Molecular Mass and Isoelectric Point of Plant Proteomes

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