Selenoprotein W gene expression in the gastrointestinal tract of chicken is affected by dietary selenium

Li, Jinlong; Li, Huixin; Li, Shu; Jiang, Zhihui; Xu, Shiwen; Tang, Zhaoxin

doi:10.1007/s10534-010-9395-0

Cited by 29 publications

(22 citation statements)

References 44 publications

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“…In our previous study, we demonstrated that SelW is expressed widely in chicken tissues, with predominant expression in the neural tissue and muscle tissue (Li et al 2011b). SelW gene expression in the avian neural tissues is sensitive to dietary Se content (unpublished data), consistent with these studies about the avian gastrointestinal tract (Li et al 2011a). In this study, we observed the mRNA expression of SelW in chicken embryos neurons and the effects of different concentrations Se on the morphology and viability of cells.…”

Section: Discussionsupporting

confidence: 91%

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Effects of Selenoprotein W gene expression by selenium involves regulation of mRNA stability in chicken embryos neurons

et al. 2012

Biometals

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Selenium (Se) and Selenoprotein W (SelW) plays a pivotal role in the brain development, function, and degeneration and that SelW expression in the brain may be affected by Se. However, the mechanism which Se regulates the SelW gene expression in neurons remains to be unclear. To investigate the effects of the SelW gene expression and mRNA stability induced by Se, primary cultured chicken embryos neurons derived from 8-day-old chick embryo cerebral hemispheres were treated with 10(-9)-10(-5) mol/l Se as selenite for 3, 6, 12, 24 or 48 h, respectively. The morphology and viability of Neurons was detected. The SelW mRNA expression level and mRNA half-life was examined in Se-treated neurons. The relative low concentrations of Se enhanced the neurite outgrowth, increased the SelW mRNA levels and elevated the mRNA half-life of chick embryo neurons. In contrast, the high concentrations of Se presented neurotoxic to neurons, decreased the SelW mRNA levels and reduced the mRNA half-life of neuronal cells. These results suggest that the alteration of post-transcriptional stabilization of SelW mRNA is an important mechanism of Se-induced the elevation or reduction of the SelW expression level in chick embryo neurons.

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Section: Discussionsupporting

confidence: 91%

“…Design of the specific primers for SelW and GADPH were performed as described previously (Li et al 2011a). Quantitative real-time PCR was performed on an ABI PRISM 7500 Detection System (Applied Biosystems, USA).…”

Section: Measurement Of Neurite Outgrowthmentioning

confidence: 99%

Effects of Selenoprotein W gene expression by selenium involves regulation of mRNA stability in chicken embryos neurons

et al. 2012

Biometals

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“…Therefore, it was showed that Se does not preferentially accumulate in muscle tissues in chicken. Se was absorbed in priority in the gastrointestinal tract of chicken, therefore, the content of Se in most tissues of the gastrointestinal tract of chicken especially in small intestine was higher than that of muscle tissues (Li et al 2010). This data indicated that the preferential accumulation of Se was in blood and the gastrointestinal tract compared with muscle tissues in chicken.…”

Section: Discussionmentioning

confidence: 75%

“…SelW mRNA expression in the gastrointestinal tract, liver, pancreatic tissue; immune organs and skeletal muscle system in chickens are highly sensitive to Se supply (Li et al 2010;Gao et al 2011;Sun et al 2011;Wang et al 2011;Yu et al 2011;Ruan et al 2011). Pagmantidis et al (2005) reported that SelN and SelW expression levels in human intestinal Caco-2 cells is highly sensitive to Se-depletion and Seadequate conditions.…”

Section: Discussionmentioning

confidence: 99%

“…In previous work, the chicken SelW cDNA has been sequenced and it was found that SelW is expressed ubiquitously in various tissues (Li et al 2010). Like SelN, SelW is highly expressed in proliferating myoblasts, decreasing during differentiation, and accordingly, it was shown to protect proliferative myoblasts from oxidative stress during muscle growth and differentiation (Loflin et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Dietary selenium regulation of transcript abundance of selenoprotein N and selenoprotein W in chicken muscle tissues

Zhang

Huang

et al. 2011

Biometals

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Selenium (Se), selenoprotein N (SelN) and selenoprotein W (SelW) play a crucial role in muscle disorders. Se status highly regulates selenoprotein mRNA levels. However, few attempts have been performed on the effect of dietary Se supplementation on muscle SelN and SelW mRNA levels in birds. To investigate the effects of Se on the regulation of SelN and SelW mRNA levels in muscle tissues, one-day-old male chickens were fed either a commercial diet or a Se-supplemented diet containing 1.0, 2.0, 3.0 or 5.0 mg/kg sodium selenite for 90 days. Muscle tissues (breast, flight, thigh, shank and cardiac muscles) were collected and examined for Se content and mRNA levels of SelN and SelW. Moreover, Selenophosphate synthetase-1 (SPS-1) and selenocysteine-synthase (SecS) mRNA levels were analyzed. Significant increases in SelN mRNA levels were obtained in breast, thigh and shank muscles treated with Se, with maximal effects at 3.0 mg Se/kg diet, but 2.0 mg Se/kg diet resulted in peak levels of Sel N mRNA in flight muscles. Changes in SelW mRNA abundance in thigh and shank muscles increased in response to Se supply. After reaching a maximal level, higher Se supplementation led to a reduction in both SelN and SelW mRNAs. However, SelN and SelW mRNA levels displayed a different expression pattern in different skeletal and cardiac muscles. Thus, it suggested that skeletal and cardiac muscles SelN and SelW mRNA levels were highly regulated by Se supplementation and different muscle tissues showed differential sensitivity. Moreover, Se supplementation also regulated the levels of SPS1 and SecS mRNAs. The mRNA levels of SPS1 and SecS were enhanced in the Se supplemented groups. These data indicate that Se regulates the expression of SelN and SelW gene and affect the mRNA levels of SecS and SPS1.

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Effects of different selenium levels on growth and regulation of laccase and versatile peroxidase in white-rot fungus, Pleurotus eryngii

Kim¹,

Lee²,

Kwon³

et al. 2014

World J Microbiol Biotechnol

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This study was conducted to evaluate the physiological effects of different selenium (Se) levels on the growth of white-rot fungus, Pleurotus eryngii, with special reference to the regulation of ligninolytic enzymes such as laccase and versatile peroxidase. The fungus was grown in medium supplemented with 1, 10, 100, 1,000 and 10,000 μM of sodium selenite. Mycelial growth was stronger at lower Se levels, but declined significantly at higher concentrations of 1,000 and 10,000 μM, highlighting its association in mediating toxic responses. Inhibition of fungal growth was accompanied with dense and entangled hyphae taking the shape of irregular short strips. Additionally, hyphal swellings and septation were noticed which lead to a reduction in the advancement of the mycelium. Along with the inhibition of fungal biomass, the reducing sugar and protein concentrations increased to about 30.2 and 3.5 mg/ml respectively in the growth medium. Additionally, the laccase gene expression showed a twofold upregulation at higher levels of Se, although the activity of the enzyme was compromised with an inverse relationship with increased gene transcripts. The versatile peroxidase transcript showed a complete downregulation at 10,000 μM after an upregulation at lower levels of Se. We also confirmed the direct relationship of different Se levels on laccase activity of Rhus vernicifera that showed similar behavior to the fungal laccase. The results of the present study suggest that Se supplementation regulates mRNA levels of laccase and versatile peroxidase depending on exposure and may play a role in the toxicity associated with Se.

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Selenoprotein W gene expression in the gastrointestinal tract of chicken is affected by dietary selenium

Cited by 29 publications

References 44 publications

Effects of Selenoprotein W gene expression by selenium involves regulation of mRNA stability in chicken embryos neurons

Effects of Selenoprotein W gene expression by selenium involves regulation of mRNA stability in chicken embryos neurons

Dietary selenium regulation of transcript abundance of selenoprotein N and selenoprotein W in chicken muscle tissues

Effects of different selenium levels on growth and regulation of laccase and versatile peroxidase in white-rot fungus, Pleurotus eryngii

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