Self-Assembled and Nanostructured siRNA Delivery Systems

Jeong, Ji Hoon; Park, Tae Gwan; Kim, Sun Hwa

doi:10.1007/s11095-011-0412-y

Cited by 51 publications

(30 citation statements)

References 94 publications

(95 reference statements)

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“…Nanotechnology based formulations have demonstrated great unique advantages in terms of their affinity, efficacy, and targetability over conventional dosage forms [6–11]. In this view, formulation scientists have also developed various theories and strategies for improvement of productivity, quality, safety, and elegance of nanosize pharmaceutical products [12–16].…”

Section: Introductionmentioning

confidence: 99%

Investigations on Agglomeration and Haemocompatibility of Vitamin E TPGS Surface Modified Berberine Chloride Nanoparticles

Vuddanda

Rajamanickam

Yaspal

et al. 2014

BioMed Research International

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The objective of the present study is to investigate the influence of surface modification on systemic stability of NPs. Vitamin E TPGS (1% w/v) was used for surface modification of berberine chloride nanoparticles. Naked and surface modified NPs were incubated in different SBFs (pH 6.8 and 7.4) with or without bile salts and human plasma. NPs were observed for particle agglomeration and morphology by particle size analyzer and TEM, respectively. The haemocompatibility studies were conducted on developed NPs to evaluate their safety profile. The surface modified NPs were stable compared to naked NPs in different SBFs due to the steric stabilization property of vitamin E TPGS. Particle agglomeration was not seen when NPs were incubated in SBF (pH 6.8) with bile salts. No agglomeration was observed in NPs after their incubation in plasma but particle size of the naked NPs increased due to adhesion of plasma proteins. The TEM images confirmed the particle size results. DSC and FT-IR studies confirmed the coexistence of TPGS in surface modified NPs. The permissible haemolysis, LDH release, and platelet aggregation revealed that NPs were compatible for systemic administration. Thus, the study illustrated that the surface modification is helpful in the maintenance of stability of NPs in systemic conditions.

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Section: Introductionmentioning

confidence: 99%

Investigations on Agglomeration and Haemocompatibility of Vitamin E TPGS Surface Modified Berberine Chloride Nanoparticles

Vuddanda

Rajamanickam

Yaspal

et al. 2014

BioMed Research International

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“…However, there has been little success in developing PECs for oligonucleotide delivery because complexation typically results in formation of large and unstable polyplexes under physiological conditions. [54,55] Structural differences between pDNA and oligonucleotides, like flexibility ( e.g. , persistence length ssDNA l p ≈ .6 nm versus dsDNA l p ≈ 53 nm[56]) and charge density ( e.g.…”

Section: Bulk Polyelectrolyte Complexesmentioning

confidence: 99%

Bulk and nanoscale polypeptide based polyelectrolyte complexes

Marciel

Chung

Brettmann

et al. 2017

Advances in Colloid and Interface Science

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Polyelectrolyte complexes (PECs) formed using polypeptides have great potential for developing new self-assembled materials, in particular for the development of drug and gene delivery vehicles. This review discusses the latest advancements in PECs formed using polypeptides as the polyanion and/or the polycation in both polyelectrolyte complexes that form bulk materials and block copolymer complexes that form nanoscale assemblies such as PEC micelles and other self-assembled structures. We highlight the importance of secondary structure formation between homogeneous polypeptide complexes, which, unlike PECs formed using other polymers, introduces additional intermolecular interactions in the form of hydrogen bonding, which may influence precipitation over coacervation. However, we still include heterogeneous complexes consisting of polypeptides and other polymers such as nucleic acids, sugars, and other synthetic polyelectrolytes. Special attention is given to complexes formed using nucleic acids as polyanions and polypeptides as polycations and their potential for delivery applications.

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“…51 Finally, lipoplexes formed by the self-assembly of nucleic acids and positively charged liposomes were applied in many studies to deliver oligonucleotides or plasmids to tumor blood vessel endothelium. 52,53 Santel et al 54 showed that liposomes prepared with a mixture of cationic and fusogenic lipids, and complexed with negatively charged small interfering ribonucleic acid effectively targeted the small interfering ribonucleic acid to tumor vascular endothelium. After intravenous injection, endothelial cell-specific uptake was confirmed in the tumor tissue, and the silencing of endothelium-specific genes encoding of CD31 and Tie2 was observed.…”

Section: Targeting Of Endothelial Cells Using Positively Charged Lipomentioning

confidence: 99%

Molecular targeting of liposomal nanoparticles to tumor microenvironment

Zhao¹,

Rodriguez²

2012

IJN

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Liposomes are biodegradable and can be used to deliver drugs at a much higher concentration in tumor tissues than in normal tissues. Both passive and active drug delivery by liposomal nanoparticles can significantly reduce the toxic side effects of anticancer drugs and enhance the therapeutic efficacy of the drugs delivered. Active liposomal targeting to tumors is achieved by recognizing specific tumor receptors through tumor-specific ligands or antibodies coupled onto the surface of the liposomes, or by stimulus-sensitive drug carriers such as acid-triggered release or enzyme-triggered drug release. Tumors are often composed of tumor cells and nontumor cells, which include endothelial cells, pericytes, fibroblasts, stromal, mesenchymal cells, innate, and adaptive immune cells. These nontumor cells thus form the tumor microenvironment, which could be targeted and modified so that it is unfavorable for tumor cells to grow. In this review, we briefly summarized articles that had taken advantage of liposomal nanoparticles as a carrier to deliver anticancer drugs to the tumor microenvironment, and how they overcame obstacles such as nonspecific uptake, interaction with components in blood, and toxicity. Special attention is devoted to the liposomal targeting of anticancer drugs to the endothelium of tumor neovasculature, tumor associated macrophages, fibroblasts, and pericytes within the tumor microenvironment.

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Self-Assembled and Nanostructured siRNA Delivery Systems

Cited by 51 publications

References 94 publications

Investigations on Agglomeration and Haemocompatibility of Vitamin E TPGS Surface Modified Berberine Chloride Nanoparticles

Investigations on Agglomeration and Haemocompatibility of Vitamin E TPGS Surface Modified Berberine Chloride Nanoparticles

Bulk and nanoscale polypeptide based polyelectrolyte complexes

Molecular targeting of liposomal nanoparticles to tumor microenvironment

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