Self-Assembled Nanogels of Cholesterol-Bearing Hydroxypropyl Cellulose: A Thermoresponsive Building Block for Nanogel Tectonic Materials

Tahara, Yoshiro; Sakiyama, Mizuki; Takeda, Shuji; Nishimura, Tomoki; Mukai, Sonoyo; Sawada, Shin–ichi; Sasaki, Yoshihiro; Akiyoshi, Kazunari

doi:10.1021/acs.langmuir.6b02406

Cited by 26 publications

(15 citation statements)

References 28 publications

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“…Chemical modification of hydrophobic groups to water soluble polymers has been reported. For example, Akiyoshi et al used hydrogel biomaterials such as cholesterol-bearing pullulan (CHP) [ 14 ], cholesterol-bearing poly-L-lysine (CHPLL) [ 15 ], cholesterol-bearing hydroxypropyl cellulose [ 16 ], and cholesterol-bearing xyloglucan [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Cholesteryl Conjugated Lysozyme (CHLysozyme)

et al. 2020

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Hydrophobic interaction is important for protein conformation. Conjugation of a hydrophobic group can introduce intermolecular hydrophobic contacts that can be contained within the molecule. It is possible that a strongly folded state can be formed in solution compared with the native state. In this study, we synthesized cholesteryl conjugated lysozyme (CHLysozyme) using lysozyme and cholesterol as the model protein and hydrophobic group, respectively. Cholesteryl conjugation to lysozyme was confirmed by nuclear-magnetic resonance. Differential-scanning calorimetry suggested that CHLysozyme was folded in solution. CHLysozyme secondary structure was similar to lysozyme, although circular dichroism spectra indicated differences to the tertiary structure. Fluorescence measurements revealed a significant increase in the hydrophobic surface of CHLysozyme compared with that of lysozyme; CHLysozyme self-associated by hydrophobic interaction of the conjugated cholesterol but the hydrophobic surface of CHLysozyme decreased with time. The results suggested that hydrophobic interaction changed from intramolecular interaction to an intermolecular interaction. Furthermore, the relative activity of CHLysozyme to lysozyme increased with time. Therefore, CHLysozyme likely forms a folded state with an extended durability of activity. Moreover, lysozyme was denatured in 100% DMSO but the local environment of tryptophan in CHLysozyme was similar to that of a native lysozyme. Thus, this study suggests that protein solution stability and resistance to organic solvents may be improved by conjugation of a hydrophobic group.

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Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Cholesteryl Conjugated Lysozyme (CHLysozyme)

et al. 2020

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“…Different hydrophobic materials are used to conjugate on hydrophilic units to form thermosensitive materials. Many studies have been performed based on this approach, and different hydrophobic materials, such as cholesterol [ 184 , 185 ], poly L-lactide (PLLA) [ 186 , 187 , 188 , 189 ], beta glycerophosphate (β-GP) [ 190 , 191 ] and pluronic F127 (F127) [ 192 , 193 ] have been used to synthesize thermosensitive hydrogels and nanogels for biomedical applications.…”

Section: Thermosensitive Polymersmentioning

confidence: 99%

“…Thara et al prepared a self-assembled thermosensitive nanogel using cholesterol as a thermosensitive agent bearing hydroxypropyl cellulose (HP-Clu) with LCST of 50 °C. The diameter of nanogels was about 100 nm and 1500 nm at 37 °C and 60 °C in PBS, respectively [ 185 ]. In another study, Fujioka et al synthesized cholesterol-bearing Plu nanogel for the delivery of bone morphogenetic protein-2 (BMP2) and Fibroblast growth factor-18 (FGF18) delivery.…”

Section: Thermosensitive Polymersmentioning

confidence: 99%

Thermoresponsive Nanogels Based on Different Polymeric Moieties for Biomedical Applications

Ghaeini-Hesaroeiye

Bagtash

Boddohi

et al. 2020

Gels

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Nanogels, or nanostructured hydrogels, are one of the most interesting materials in biomedical engineering. Nanogels are widely used in medical applications, such as in cancer therapy, targeted delivery of proteins, genes and DNAs, and scaffolds in tissue regeneration. One salient feature of nanogels is their tunable responsiveness to external stimuli. In this review, thermosensitive nanogels are discussed, with a focus on moieties in their chemical structure which are responsible for thermosensitivity. These thermosensitive moieties can be classified into four groups, namely, polymers bearing amide groups, ether groups, vinyl ether groups and hydrophilic polymers bearing hydrophobic groups. These novel thermoresponsive nanogels provide effective drug delivery systems and tissue regeneration constructs for treating patients in many clinical applications, such as targeted, sustained and controlled release.

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“…The results showed adjustable drug transport kinetics and a temperature‐controlled long‐term diclofenac transport due to the gel matrix. Hydroxypropyl cellulose (HPC), another polysaccharide known to have thermal response, was also used in developing nanogel‐integrated hydrogels . HPC was modified with cholesteryl groups for the formation of the nanogel by self‐assembly through hydrophobic interaction in water cholesteryl Hydroxypropyl cellulose (Ch‐HPC).…”

Section: Nanogel‐integrated Hydrogelmentioning

confidence: 99%

Advancement in the Biomedical Applications of the (Nano)gel Structures Based on Particular Polysaccharides

Chiriac¹,

Ghilan²,

Neamţu³

et al. 2019

Macromolecular Bioscience

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The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/mabi.201900187. Nanogels (Nano)gels from macromolecular compounds-natural, synthetic, or a combination thereof, suitable crosslinkers-and conferred characteristicssuch as degradability, size, charge, amphiphilicity, responsiveness, and softness-are capable of responding to the challenges imposed by bioengineering applications. Polysaccharide-based gels have received particular attention in this field. This review addresses recent advancement in the use of (nano)gel structures prepared only from compounds based on gellan gum, heparin, chondroitin sulfate, carrageenan, guar gum, galactose, or agarose, which represent an important part of the special class of natural polymers, the polysaccharides. Also, future trends are taken into discussion regarding the (nano)gels' use in biomedical applications such as biomimetics, biosensors, artificial muscles, and chemical separations in relation with their ability to be used as a vehicle for various biomolecules due to their physicochemical properties, biocompatibility, and biodegradability.

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Self-Assembled Nanogels of Cholesterol-Bearing Hydroxypropyl Cellulose: A Thermoresponsive Building Block for Nanogel Tectonic Materials

Cited by 26 publications

References 28 publications

Synthesis and Characterization of Cholesteryl Conjugated Lysozyme (CHLysozyme)

Synthesis and Characterization of Cholesteryl Conjugated Lysozyme (CHLysozyme)

Thermoresponsive Nanogels Based on Different Polymeric Moieties for Biomedical Applications

Advancement in the Biomedical Applications of the (Nano)gel Structures Based on Particular Polysaccharides

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