Self-assembly and immobilization of liposomes in fused-silica capillary by avidin–biotin binding

δ-Free FoF1-ATPase, Nanomachine and Biosensor

“…Thermophilic bacterium, Bacillus PS3β 10×his-tag subunit (TF 1 β), and F 1 -ATPase (α 3 β (10×his-tag)3 γ) were expressed and purified as Ref. (Yang et al, 1998), in which the JM103 strain expressing F 1 -ATPase was cultured in 2× YT medium (AMP + ) for 3-4 h at 37 • C. When the A 660 increased to 0.6-0.8, the expression of the F 1 -ATPase was induced by addition of 1 mmol/L isopropylthio-β-D-galactoside for 3 h. Cells were harvested by centrifuging for 15 min at 4000g and cell extractions were prepared using lysozyme (1 mg/mL)/sonication (5 min) in 50 mmol/L Tris-HCl (pH 8.0) buffer containing 0.5 mol/L NaCl and 1 mmol/L phenylmethane sulfonyl fluoride. The extracts were incubated at 60 • C for 30 min, and TF 1 β was purified using Ni 2+ -NTA affinity chromatography at 4 • C. F 1 -ATPase (α 3 β (10×his-tag)3 γ) was purified as Ref.…”

“…(Montemagno & Bachand, 1999) at 25 • C. The F o F 1 -ATP synthase from the E. coli JM103(uncB-UncD) was purified as Ref. (Yang et al, 1998). The mutant ATP synthase containing the His-tag could be isolated with Ni-NTA column.…”

Progress in fabricating arrays of soft spherical vessels on mesoscale with spatial control

“…Avidin-biotin is the most commonly used receptorligand pair for specific binding of phospholipid vesicles onto a substrate [55][56][57] . Generally, the avidin is patterned onto the surface of the substrate, and the biotin is modified on the lipid headgroups extending out from the vesicle face, such that the biotinylated vesicles can be tightly coupled to the avidin region on the solid surface via the biological affinity.…”

Lipid vesicles in capillary electrophoretic techniques: Characterization of structural properties and associated membrane‐molecule interactions

“…Intact lipid vesicles can be immobilized in a capillary to create a stationary phase as well. An avidinbiotin linkage has been used to attach multiple layers of lipid vesicles to the inner walls of a fused-silica capillary [108]. The inner walls of the capillary may also be derivatized with a positively charged polymer, such as a derivatized agarose.…”